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Fly Volume 11, 2017 - Issue 1
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Methods and Technical Advances

Tools and strategies for scarless allele replacement in Drosophila using CRISPR/Cas9

Abigail M. LambDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI, USA
,
Elizabeth A. WalkerDepartment of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI, USA
&
Patricia J. WittkoppDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI, USA;Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI, USACorrespondence[email protected]
Pages 53-64 | Received 09 May 2016, Accepted 21 Jul 2016, Published online: 22 Aug 2016

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Kevin G. Nyberg, Joseph Q. Nguyen, Yong-Jae Kwon, Shelby Blythe, Greg J. Beitel & Richard Carthew. (2020) A pipeline for precise and efficient genome editing by sgRNA-Cas9 RNPs in Drosophila. Fly 14:1-4, pages 34-48.
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J. Komal, H. R. Desai, Ipsita Samal, Andrea Mastinu, R. D. Patel, P. V. Dinesh Kumar, Prasanta Kumar Majhi, Deepak Kumar Mahanta & Tanmaya Kumar Bhoi. (2023) Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management. Plants 12:23, pages 3961.
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Cansu Kose, Xuemei Cao, Evan B Dewey, Mustafa Malkoç, Ogün Adebali, Jeff Sekelsky, Laura A Lindsey-Boltz & Aziz Sancar. (2023) Cross-species investigation into the requirement of XPA for nucleotide excision repair. Nucleic Acids Research.
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Xueying C. Li, Timothy Fuqua, Maria Elize van Breugel & Justin Crocker. (2023) Mutational scans reveal differential evolvability of Drosophila promoters and enhancers . Philosophical Transactions of the Royal Society B: Biological Sciences 378:1877.
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Khoi Huynh, Brittny R. Smith, Stuart J. Macdonald & Anthony D. Long. (2023) Genetic variation in chromatin state across multiple tissues in Drosophila melanogaster. PLOS Genetics 19:5, pages e1010439.
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Tasfia Tasnim Moon, Ishrat Jahan Maliha, Abdullah Al Moin Khan, Moutoshi Chakraborty, Md Sharaf Uddin, Md Ruhul Amin & Tofazzal Islam. (2022) CRISPR-Cas Genome Editing for Insect Pest Stress Management in Crop Plants. Stresses 2:4, pages 493-514.
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Stuart J Macdonald & Anthony D Long. (2022) Discovery of malathion resistance QTL in Drosophila melanogaster using a bulked phenotyping approach . G3 Genes|Genomes|Genetics 12:12.
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Sanchita Singh, Somnath Rahangdale, Shivali Pandita, Gauri Saxena, Santosh Kumar Upadhyay, Geetanjali Mishra & Praveen C. Verma. (2022) CRISPR/Cas9 for Insect Pests Management: A Comprehensive Review of Advances and Applications. Agriculture 12:11, pages 1896.
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Susan T Harbison. (2022) What have we learned about sleep from selective breeding strategies?. Sleep 45:11.
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Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie E. Mohr & Norbert Perrimon. (2022) State-of-the-art CRISPR for in vivo and cell-based studies in Drosophila. Trends in Genetics 38:5, pages 437-453.
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Stuart J Macdonald, Kristen M Cloud-Richardson, Dylan J Sims-West & Anthony D Long. (2022) Powerful, efficient QTL mapping in Drosophila melanogaster using bulked phenotyping and pooled sequencing . Genetics 220:3.
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Florian P. Fischer, Ameya S. Kasture, Thomas Hummel & Sonja Sucic. (2022) Molecular and Clinical Repercussions of GABA Transporter 1 Variants Gone Amiss: Links to Epilepsy and Developmental Spectrum Disorders. Frontiers in Molecular Biosciences 9.
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Jun Xu, Ah-Ram Kim, Ross W Cheloha, Fabian A Fischer, Joshua Shing Shun Li, Yuan Feng, Emily Stoneburner, Richard Binari, Stephanie E Mohr, Jonathan Zirin, Hidde L Ploegh & Norbert Perrimon. (2022) Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodies. eLife 11.
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Daisy J. Vinter, Caroline Hoppe & Hilary L. Ashe. (2021) Live and fixed imaging of translation sites at single mRNA resolution in the Drosophila embryo. STAR Protocols 2:3, pages 100812.
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Stephanie E Mohr, Sudhir Gopal Tattikota, Jun Xu, Jonathan Zirin, Yanhui Hu & Norbert Perrimon. (2021) Methods and tools for spatial mapping of single-cell RNAseq clusters in Drosophila . Genetics 217:4.
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Caroline Hoppe & Hilary L. Ashe. (2021) CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos. STAR Protocols 2:1, pages 100380.
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Vittoria Mariano, Tilmann Achsel, Claudia Bagni & Alexandros K. Kanellopoulos. (2020) Modelling Learning and Memory in Drosophila to Understand Intellectual Disabilities. Neuroscience 445, pages 12-30.
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Marliette R. Matos, Seok-Man Ho, Nadine Schrode & Kristen J. Brennand. (2020) Integration of CRISPR-engineering and hiPSC-based models of psychiatric genomics. Molecular and Cellular Neuroscience 107, pages 103532.
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Benjamin J. Matthews & Leslie B. Vosshall. (2020) How to turn an organism into a model organism in 10 ‘easy’ steps. Journal of Experimental Biology 223:Suppl_1.
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V. Edwin Hillary, Stanislaus Antony Ceasar & S. Ignacimuthu. 2020. Genome Engineering via CRISPR-Cas9 System. Genome Engineering via CRISPR-Cas9 System 219 249 .
Aaron J Storey, Hsin-Ping Wang, Reine U Protacio, Mari K Davidson & Wayne P Wahls. (2019) Targeted Forward Genetics: Population-Scale Analyses of Allele Replacements Spanning Thousands of Base Pairs in Fission Yeast. G3 Genes|Genomes|Genetics 9:12, pages 4097-4106.
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David Li-Kroeger, Oguz Kanca, Pei-Tseng Lee, Sierra Cowan, Michael T Lee, Manish Jaiswal, Jose Luis Salazar, Yuchun He, Zhongyuan Zuo & Hugo J Bellen. (2018) An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila. eLife 7.
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Ethan Bier, Melissa M Harrison, Kate M O’Connor-Giles & Jill Wildonger. (2018) Advances in Engineering the Fly Genome with the CRISPR-Cas System. Genetics 208:1, pages 1-18.
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Dagmara Korona, Stefan Koestler & Steven Russell. (2017) Engineering the Drosophila Genome for Developmental Biology. Journal of Developmental Biology 5:4, pages 16.
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Dan Sun, Zhaojiang Guo, Yong Liu & Youjun Zhang. (2017) Progress and Prospects of CRISPR/Cas Systems in Insects and Other Arthropods. Frontiers in Physiology 8.
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