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Jennifer M. Nguyen, Jacquelynn Smith, Susan Rzewuski, Cristina Legido-Quigley & Matthew A. Lauber. (2019) High sensitivity LC-MS profiling of antibody-drug conjugates with difluoroacetic acid ion pairing. mAbs 11:8, pages 1358-1366.
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Olga V. Friese, Jacquelynn N. Smith, Paul W. Brown & Jason C. Rouse. (2018) Practical approaches for overcoming challenges in heightened characterization of antibody-drug conjugates with new methodologies and ultrahigh-resolution mass spectrometry. mAbs 10:3, pages 335-345.
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Anil Wagh, Hangtian Song, Ming Zeng, Li Tao & Tapan K. Das. (2018) Challenges and new frontiers in analytical characterization of antibody-drug conjugates. mAbs 10:2, pages 222-243.
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Liuxi Chen, Lan Wang, Henry Shion, Chuanfei Yu, Ying Qing Yu, Lei Zhu, Meng Li, Weibin Chen & Kai Gao. (2016) In-depth structural characterization of Kadcyla® (ado-trastuzumab emtansine) and its biosimilar candidate. mAbs 8:7, pages 1210-1223.
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Robert E. Birdsall, Sean M. McCarthy, Marie Claire Janin-Bussat, Michel Perez, Jean-François Haeuw, Weibin Chen & Alain Beck. (2016) A sensitive multidimensional method for the detection, characterization, and quantification of trace free drug species in antibody-drug conjugate samples using mass spectral detection. mAbs 8:2, pages 306-317.
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Articles from other publishers (49)
Raya Sadighi, Vera de Kleijne, Sam Wouters, Karin Lubbers, Govert W. Somsen, Andrea F.G. Gargano & Rob Haselberg. (2024) Online multimethod platform for comprehensive characterization of monoclonal antibodies in cell culture fluid from a single sample injection - Intact protein workflow. Analytica Chimica Acta 1287, pages 342074.
Crossref
Crossref
Ettore Gilardoni & Luca Regazzoni. (2022) Liquid phase separation techniques for the characterization of monoclonal antibodies and bioconjugates. Journal of Chromatography Open 2, pages 100034.
Crossref
Crossref
Liesa Verscheure, Gerd Vanhoenacker, Sonja Schneider, Tom Merchiers, Julie Storms, Pat Sandra, Frederic Lynen & Koen Sandra. (2022)
3D-LC-MS with
2
D Multimethod Option for Fully Automated Assessment of Multiple Attributes of Monoclonal Antibodies Directly from Cell Culture Supernatants
. Analytical Chemistry 94:17, pages 6502-6511.
Crossref
Crossref
Marco Cavaco, Miguel A. R. B. Castanho & Vera Neves. (2022) The Use of Antibody-Antibiotic Conjugates to Fight Bacterial Infections. Frontiers in Microbiology 13.
Crossref
Crossref
Florian Füssl, Conor S. Barry, Kathryn M. Pugh, K. Phin Chooi, Balakumar Vijayakrishnan, Gyoung-Dong Kang, Christina von Bulow, Philip W. Howard & Jonathan Bones. (2021) Simultaneous monitoring of multiple attributes of pyrrolobenzodiazepine antibody-drug conjugates by size exclusion chromatography – high resolution mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 205, pages 114287.
Crossref
Crossref
Shino Manabe & Kenji Hirose. (2021) Chemistry in ADC Development抗体－薬物複合体（ADC）に活用された化学. Drug Delivery System 36:1, pages 28-39.
Crossref
Crossref
Tobias Graf, Michael Leiss, Markus Haberger & Patrick Bulau. 2021. Monoclonal Antibodies. Monoclonal Antibodies
199
234
.
Tobias Graf, Katrin Heinrich, Ingrid Grunert, Harald Wegele, Markus Haberger, Patrick Bulau & Michael Leiss. (2020) Recent advances in LC–MS based characterization of protein-based bio-therapeutics – mastering analytical challenges posed by the increasing format complexity. Journal of Pharmaceutical and Biomedical Analysis 186, pages 113251.
Crossref
Crossref
Xiaoyu Zhu, Shihan Huo, Chao Xue, Bo An & Jun Qu. (2020) Current LC-MS-based strategies for characterization and quantification of antibody-drug conjugates. Journal of Pharmaceutical Analysis 10:3, pages 209-220.
Crossref
Crossref
Evelin Farsang, Davy Guillarme, Jean-Luc Veuthey, Alain Beck, Matthew Lauber, Andrew Schmudlach & Szabolcs Fekete. (2020) Coupling non-denaturing chromatography to mass spectrometry for the characterization of monoclonal antibodies and related products. Journal of Pharmaceutical and Biomedical Analysis 185, pages 113207.
Crossref
Crossref
Brittney J. Mills, Terra Kruger, Milan Bruncko, Xinxin Zhang & Feroz Jameel. (2020) Effect of Linker-Drug Properties and Conjugation Site on the Physical Stability of ADCs. Journal of Pharmaceutical Sciences 109:5, pages 1662-1672.
Crossref
Crossref
Soraya Chapel, Florent Rouvière, Morgan Sarrut & Sabine Heinisch. 2020. Antibody-Drug Conjugates. Antibody-Drug Conjugates
163
185
.
Alexandre Goyon, Michael Kim, Lu Dai, Christopher Cornell, Fred Jacobson, Davy Guillarme & Cinzia Stella. (2019) Streamlined Characterization of an Antibody–Drug Conjugate by Two-Dimensional and Four-Dimensional Liquid Chromatography/Mass Spectrometry. Analytical Chemistry 91:23, pages 14896-14903.
Crossref
Crossref
Ke Li, Zhongping John Lin, Honglan Shi & Yinfa Ma. (2019) Characterization of Positional Isomers of Interchain Cysteine Linked Antibody−Drug Conjugates by High-Resolution Mass Spectrometry. Analytical Chemistry 91:13, pages 8558-8563.
Crossref
Crossref
Jianyao Wang, Wei Zhang, Rhys Salter & Heng-Keang Lim. (2019) Reductive Desulfuration as an Important Tool in Detection of Small Molecule Modifications to Payload of Antibody Drug Conjugates. Analytical Chemistry 91:3, pages 2368-2375.
Crossref
Crossref
Shino Manabe. (2019) Development and Current Status of Antibody-drug conjugate (ADC)抗体－薬物複合体開発の発展と現状. Drug Delivery System 34:1, pages 10-21.
Crossref
Crossref
Tsutomu Arakawa. (2018) Review on the Application of Mixed-mode Chromatography for Separation of Structure Isoforms. Current Protein & Peptide Science 20:1, pages 56-60.
Crossref
Crossref
Malin Källsten, Rafael Hartmann, Konstantin Artemenko, Sara Bergström Lind, Fredrik Lehmann & Jonas Bergquist. (2018) Qualitative analysis of antibody–drug conjugates (ADCs): an experimental comparison of analytical techniques of cysteine-linked ADCs. The Analyst 143:22, pages 5487-5496.
Crossref
Crossref
David Pfister, Lucrèce Nicoud & Massimo Morbidelli. 2018. Continuous Biopharmaceutical Processes. Continuous Biopharmaceutical Processes.
Jakob W. Buecheler, Matthias Winzer, Jason Tonillo, Christian Weber & Henning Gieseler. (2018) Impact of Payload Hydrophobicity on the Stability of Antibody–Drug Conjugates. Molecular Pharmaceutics 15:7, pages 2656-2664.
Crossref
Crossref
Anthony Ehkirch, Valentina D’Atri, Florent Rouviere, Oscar Hernandez-Alba, Alexandre Goyon, Olivier Colas, Morgan Sarrut, Alain Beck, Davy Guillarme, Sabine Heinisch & Sarah Cianferani. (2018) An Online Four-Dimensional HIC×SEC-IM×MS Methodology for Proof-of-Concept Characterization of Antibody Drug Conjugates. Analytical Chemistry 90:3, pages 1578-1586.
Crossref
Crossref
Balázs Bobály, Sandrine Fleury-Souverain, Alain Beck, Jean-Luc Veuthey, Davy Guillarme & Szabolcs Fekete. (2018) Current possibilities of liquid chromatography for the characterization of antibody-drug conjugates. Journal of Pharmaceutical and Biomedical Analysis 147, pages 493-505.
Crossref
Crossref
Koen Sandra, Mieke Steenbeke, Isabel Vandenheede, Gerd Vanhoenacker & Pat Sandra. (2017) The versatility of heart-cutting and comprehensive two-dimensional liquid chromatography in monoclonal antibody clone selection. Journal of Chromatography A 1523, pages 283-292.
Crossref
Crossref
Miao Qu, Bo An, Shichen Shen, Ming Zhang, Xiaomeng Shen, Xiaotao Duan, Joseph P. Balthasar & Jun Qu. (2016) Qualitative and quantitative characterization of protein biotherapeutics with liquid chromatography mass spectrometry. Mass Spectrometry Reviews 36:6, pages 734-754.
Crossref
Crossref
John A Davis, Mark Kagan, Jessica Read, Markus Walles & Panos Hatsis. (2017)
Immunoprecipitation middle-up LC–MS for
in vivo
drug-to-antibody ratio determination for antibody–drug conjugates
. Bioanalysis 9:20, pages 1535-1549.
Crossref
Crossref
Kevin Jooß, Jens Hühner, Steffen Kiessig, Bernd Moritz & Christian Neusüß. (2017) Two-dimensional capillary zone electrophoresis–mass spectrometry for the characterization of intact monoclonal antibody charge variants, including deamidation products. Analytical and Bioanalytical Chemistry 409:26, pages 6057-6067.
Crossref
Crossref
Jonathan J. Gilroy & Catherine M. Eakin. (2017) Characterization of drug load variants in a thiol linked antibody-drug conjugate using multidimensional chromatography. Journal of Chromatography B 1060, pages 182-189.
Crossref
Crossref
Kenichiro Furuki & Toshimasa Toyo'oka. (2017) Determination of thiol-to-protein ratio and drug-to-antibody ratio by in-line size exclusion chromatography with post-column reaction. Analytical Biochemistry 527, pages 33-44.
Crossref
Crossref
Jintang He, Dian Su, Carl Ng, Luna Liu, Shang-Fan Yu, Thomas H. Pillow, Geoffrey Del Rosario, Martine Darwish, Byoung-Chul Lee, Rachana Ohri, Hongxiang Zhou, Xueji Wang, Jiawei Lu, Surinder Kaur & Keyang Xu. (2017)
High-Resolution Accurate-Mass Mass Spectrometry Enabling In-Depth Characterization of
in Vivo
Biotransformations for Intact Antibody-Drug Conjugates
. Analytical Chemistry 89:10, pages 5476-5483.
Crossref
Crossref
Reiko IizukaFrank KotchApril XuThomas PorterHenry Shion, Robert Birdsall & Weibin Chen. (2017) The Characterization of Drug-to-Antibody Ratio and Isoforms of ADCs Using LC and MS TechnologiesLC及びMS技術を用いたADCの薬物抗体比及び薬物結合位置の解析. YAKUGAKU ZASSHI 137:5, pages 525-528.
Crossref
Crossref
Abasha Williams, Erik K. Read, Cyrus D. Agarabi, Scott Lute & Kurt A. Brorson. (2017) Automated 2D-HPLC method for characterization of protein aggregation with in-line fraction collection device. Journal of Chromatography B 1046, pages 122-130.
Crossref
Crossref
Hua Sang, Gaoyuan Lu, Yazhong Liu, Qiyue Hu, Weifeng Xing, Dongbing Cui, Fang Zhou, Jingwei Zhang, Haiping Hao, Guangji Wang & Hui Ye. (2017) Conjugation site analysis of antibody-drug-conjugates (ADCs) by signature ion fingerprinting and normalized area quantitation approach using nano-liquid chromatography coupled to high resolution mass spectrometry. Analytica Chimica Acta 955, pages 67-78.
Crossref
Crossref
Maria Wendt & Guido Cappuccilli. 2017. Synthetic Antibodies. Synthetic Antibodies
237
250
.
Dian Su, Carl Ng, Mehraban Khosraviani, Shang-Fan Yu, Ely Cosino, Surinder Kaur & Keyang Xu. (2016) Custom-Designed Affinity Capture LC-MS F(ab′)2 Assay for Biotransformation Assessment of Site-Specific Antibody Drug Conjugates. Analytical Chemistry 88:23, pages 11340-11346.
Crossref
Crossref
David Pfister, Nicole Ulmer, Antoine Klaue, Oliver Ingold & Massimo Morbidelli. (2016) Modeling the Kinetics of Protein Conjugation Reactions. Chemie Ingenieur Technik 88:11, pages 1598-1608.
Crossref
Crossref
Maria Kristina Parr, Othman Montacir & Houda Montacir. (2016) Physicochemical characterization of biopharmaceuticals. Journal of Pharmaceutical and Biomedical Analysis 130, pages 366-389.
Crossref
Crossref
Szabolcs Fekete, Jean-Luc Veuthey, Alain Beck & Davy Guillarme. (2016) Hydrophobic interaction chromatography for the characterization of monoclonal antibodies and related products. Journal of Pharmaceutical and Biomedical Analysis 130, pages 3-18.
Crossref
Crossref
Tao Chen, Yan Chen, Cinzia Stella, Colin D. Medley, Jason A. Gruenhagen & Kelly Zhang. (2016) Antibody-drug conjugate characterization by chromatographic and electrophoretic techniques. Journal of Chromatography B 1032, pages 39-50.
Crossref
Crossref
Morgan Sarrut, Szabolcs Fekete, Marie-Claire Janin-Bussat, Olivier Colas, Davy Guillarme, Alain Beck & Sabine Heinisch. (2016) Analysis of antibody-drug conjugates by comprehensive on-line two-dimensional hydrophobic interaction chromatography x reversed phase liquid chromatography hyphenated to high resolution mass spectrometry. II- Identification of sub-units for the characterization of even and odd load drug species. Journal of Chromatography B 1032, pages 91-102.
Crossref
Crossref
Morgan Sarrut, Amélie Corgier, Szabolcs Fekete, Davy Guillarme, David Lascoux, Marie-Claire Janin-Bussat, Alain Beck & Sabine Heinisch. (2016) Analysis of antibody-drug conjugates by comprehensive on-line two-dimensional hydrophobic interaction chromatography x reversed phase liquid chromatography hyphenated to high resolution mass spectrometry. I − Optimization of separation conditions. Journal of Chromatography B 1032, pages 103-111.
Crossref
Crossref
Dwight Stoll, John Danforth, Kelly Zhang & Alain Beck. (2016) Characterization of therapeutic antibodies and related products by two-dimensional liquid chromatography coupled with UV absorbance and mass spectrometric detection. Journal of Chromatography B 1032, pages 51-60.
Crossref
Crossref
Koen Sandra, Gerd Vanhoenacker, Isabel Vandenheede, Mieke Steenbeke, Maureen Joseph & Pat Sandra. (2016) Multiple heart-cutting and comprehensive two-dimensional liquid chromatography hyphenated to mass spectrometry for the characterization of the antibody-drug conjugate ado-trastuzumab emtansine. Journal of Chromatography B 1032, pages 119-130.
Crossref
Crossref
Balázs Bobály, Enikő Sipkó & Jenő Fekete. (2016) Challenges in liquid chromatographic characterization of proteins. Journal of Chromatography B 1032, pages 3-22.
Crossref
Crossref
Leanne Grafmuller, Cong Wei, Ragu Ramanathan, Frank Barletta, Rick Steenwyk & Joseph Tweed. (2016) Unconjugated payload quantification and DAR characterization of antibody–drug conjugates using high-resolution MS. Bioanalysis 8:16, pages 1663-1678.
Crossref
Crossref
Cong Wei, Guodong Zhang, Tracey Clark, Frank Barletta, L. Nathan Tumey, Brian Rago, Steven Hansel & Xiaogang Han. (2016) Where Did the Linker-Payload Go? A Quantitative Investigation on the Destination of the Released Linker-Payload from an Antibody-Drug Conjugate with a Maleimide Linker in Plasma. Analytical Chemistry 88:9, pages 4979-4986.
Crossref
Crossref
Richard Y.-C. Huang & Guodong Chen. (2016) Characterization of antibody–drug conjugates by mass spectrometry: advances and future trends. Drug Discovery Today 21:5, pages 850-855.
Crossref
Crossref
Jianxin Guo, Sandeep Kumar, Mark Chipley, Olivier Marcq, Devansh Gupta, Zhaowei Jin, Dheeraj S. Tomar, Cecily Swabowski, Jacquelynn Smith, Jason A. Starkey & Satish K. Singh. (2016) Characterization and Higher-Order Structure Assessment of an Interchain Cysteine-Based ADC: Impact of Drug Loading and Distribution on the Mechanism of Aggregation. Bioconjugate Chemistry 27:3, pages 604-615.
Crossref
Crossref
Erin A. Redman, J. Scott Mellors, Jason A. Starkey & J. Michael Ramsey. (2016) Characterization of Intact Antibody Drug Conjugate Variants Using Microfluidic Capillary Electrophoresis–Mass Spectrometry. Analytical Chemistry 88:4, pages 2220-2226.
Crossref
Crossref
Szabolcs Fekete, Davy Guillarme, Pat Sandra & Koen Sandra. (2015) Chromatographic, Electrophoretic, and Mass Spectrometric Methods for the Analytical Characterization of Protein Biopharmaceuticals. Analytical Chemistry 88:1, pages 480-507.
Crossref
Crossref