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mAbs Volume 9, 2017 - Issue 5
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High-resolution mass spectrometry confirms the presence of a hydroxyproline (Hyp) post-translational modification in the GGGGP linker of an Fc-fusion protein

Chris SpahrDiscovery Attribute Sciences, Therapeutic Discovery, Amgen Inc., Thousand Oaks, CA, USACorrespondence[email protected]
ORCID Iconhttp://orcid.org/0000-0002-4093-9376
ORCID Icon,
Kannan GunasekaranBiologics Optimization, Therapeutic Discovery, Amgen Inc., Thousand Oaks, CA, USA
,
Kenneth W. WalkerBiologics Optimization, Therapeutic Discovery, Amgen Inc., Thousand Oaks, CA, USA
&
Stone D.-H. ShiDiscovery Attribute Sciences, Therapeutic Discovery, Amgen Inc., Thousand Oaks, CA, USAORCID Iconhttp://orcid.org/0000-0001-8723-830X
ORCID Icon
Pages 812-819 | Received 24 Mar 2017, Accepted 26 Apr 2017, Published online: 14 Jun 2017

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Oksana Tyshchuk, Christoph Gstöttner, Dennis Funk, Simone Nicolardi, Stefan Frost, Stefan Klostermann, Tim Becker, Elena Jolkver, Felix Schumacher, Claudia Ferrara Koller, Hans Rainer Völger, Manfred Wuhrer, Patrick Bulau & Michael Mølhøj. (2019) Characterization and prediction of positional 4-hydroxyproline and sulfotyrosine, two post-translational modifications that can occur at substantial levels in CHO cells-expressed biotherapeutics. mAbs 11:7, pages 1219-1232.
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Christopher S. Spahr, Mark E. Daris, Kevin C. Graham, Brian D. Soriano, Jennitte L. Stevens & Stone D.-H. Shi. (2018) Discovery, characterization, and remediation of a C-terminal Fc-extension in proteins expressed in CHO cells. mAbs 10:8, pages 1291-1300.
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Articles from other publishers (9)

Iain D. G. Campuzano & Wendy Sandoval. (2021) Denaturing and Native Mass Spectrometric Analytics for Biotherapeutic Drug Discovery Research: Historical, Current, and Future Personal Perspectives. Journal of the American Society for Mass Spectrometry 32:8, pages 1861-1885.
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Tobias Graf, Michael Leiss, Markus Haberger & Patrick Bulau. 2021. Monoclonal Antibodies. Monoclonal Antibodies 199 234 .
Shanta Boddapati, Jason Gilmore, Kyle Boone, John Bushey, Jonathan Ross, Brian Gfeller, William McFee, Romesh Rao, Greg Corrigan, Aaron Chen, Howard Clarke, John Valliere-Douglass & Swapnil Bhargava. (2020) Evidence for co-translational misincorporation of non-canonical amino acid hydroxyproline in recombinant antibodies produced in Chinese Hamster Ovary (CHO) cell lines. PLOS ONE 15:10, pages e0241250.
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Tobias Graf, Katrin Heinrich, Ingrid Grunert, Harald Wegele, Markus Haberger, Patrick Bulau & Michael Leiss. (2020) Recent advances in LC–MS based characterization of protein-based bio-therapeutics – mastering analytical challenges posed by the increasing format complexity. Journal of Pharmaceutical and Biomedical Analysis 186, pages 113251.
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Anna C. Robotham & John F. Kelly. 2020. Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics. Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics 1 33 .
Iain D.G. Campuzano & Kevin Giles. (2019) Historical, current and future developments of travelling wave ion mobility mass spectrometry: A personal perspective. TrAC Trends in Analytical Chemistry 120, pages 115620.
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Nick A. van Huizen, Peter C. Burgers, Fabrice Saintmont, Patrick Brocorens, Pascal Gerbaux, Christoph Stingl, Lennard J.M. Dekker, Jan N.M. IJzermans & Theo M. Luider. (2019) Identification of 4-Hydroxyproline at the Xaa Position in Collagen by Mass Spectrometry. Journal of Proteome Research 18:5, pages 2045-2051.
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Ye Hou, Hang Su, Zhiying Luo, Mingying Li, Xiaonan Ma & Ningning Ma. (2019) Nutrient Optimization Reduces Phosphorylation and Hydroxylation Level on an Fc-Fusion Protein in a CHO Fed-Batch Process. Biotechnology Journal 14:3, pages 1700706.
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Iain DG Campuzano & Jennifer L Lippens. (2018) Ion mobility in the pharmaceutical industry: an established biophysical technique or still niche?. Current Opinion in Chemical Biology 42, pages 147-159.
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