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Intein mediated high throughput screening for bispecific antibodies

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Article: 1731938 | Received 05 Dec 2019, Accepted 16 Feb 2020, Published online: 09 Mar 2020

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Huifang Zong, Lei Han, Jie Chen, Zhidi Pan, Lei Wang, Rui Sun, Kai Ding, Yueqing Xie, Hua Jiang, Huili Lu, John Gilly, Baohong Zhang & Jianwei Zhu. (2021) Kinetics study of the natural split Npu DnaE intein in the generation of bispecific IgG antibodies. Applied Microbiology and Biotechnology 106:1, pages 161-171.
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Oskar J. Lange & Karen M. Polizzi. (2021) Click it or stick it: Covalent and non-covalent methods for protein-self assembly. Current Opinion in Systems Biology 28, pages 100374.
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Hiroki Akiba, Tomoko Ise, Satoshi Nagata, Haruhiko Kamada, Hiroaki Ohno & Kouhei Tsumoto. (2021) Production of IgG1-based bispecific antibody without extra cysteine residue via intein-mediated protein trans-splicing. Scientific Reports 11:1.
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Rui Sun, Yuexian Zhou, Lei Han, Zhidi Pan, Jie Chen, Huifang Zong, Yanlin Bian, Hua Jiang, Baohong Zhang & Jianwei Zhu. (2021) A Rational Designed Novel Bispecific Antibody for the Treatment of GBM. Biomedicines 9:6, pages 640.
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Christian Hentrich, Sarah-Jane Kellmann, Mateusz Putyrski, Manuel Cavada, Hanh Hanuschka, Achim Knappik & Francisco Ylera. (2021) Periplasmic expression of SpyTagged antibody fragments enables rapid modular antibody assembly. Cell Chemical Biology 28:6, pages 813-824.e6.
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Jisoo Park, Sangwoo Lee, Yuseon Kim & Tae Hyeon Yoo. (2021) Methods to generate site-specific conjugates of antibody and protein. Bioorganic & Medicinal Chemistry 30, pages 115946.
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Manali S. Sawant, Craig N. Streu, Lina Wu & Peter M. Tessier. (2020) Toward Drug-Like Multispecific Antibodies by Design. International Journal of Molecular Sciences 21:20, pages 7496.
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Tim Hofmann, Simon Krah, Carolin Sellmann, Stefan Zielonka & Achim Doerner. (2020) Greatest Hits—Innovative Technologies for High Throughput Identification of Bispecific Antibodies. International Journal of Molecular Sciences 21:18, pages 6551.
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Anniina Jaakkonen, Gerrit Volkmann & Hideo Iwaï. (2020) An Off-the-Shelf Approach for the Production of Fc Fusion Proteins by Protein Trans-Splicing towards Generating a Lectibody In Vitro. International Journal of Molecular Sciences 21:11, pages 4011.
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