6,298
Views
67
CrossRef citations to date
0
Altmetric
Research Article

Different isolation approaches lead to diverse glycosylated extracellular vesicle populations

ORCID Icon, ORCID Icon, ORCID Icon, , ORCID Icon, , , ORCID Icon & ORCID Icon show all
Article: 1621131 | Received 27 Sep 2018, Accepted 03 May 2019, Published online: 03 Jun 2019

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (4)

Jnana A.Sadiya S. S.Satyamoorthy K.Murali T.S.. (2023) Extracellular vesicles in bacterial and fungal diseases – Pathogenesis to diagnostic biomarkers. Virulence 14:1.
Read now
Zhi Cai, Junyan Feng, Nian Dong, Pan Zhou, Yuanshuai Huang & Hongwei Zhang. (2023) Platelet-derived extracellular vesicles play an important role in platelet transfusion therapy. Platelets 34:1.
Read now
Bairen Pang, Ying Zhu, Jie Ni, Juanfang Ruan, James Thompson, David Malouf, Joseph Bucci, Peter Graham & Yong Li. (2020) Quality Assessment and Comparison of Plasma-Derived Extracellular Vesicles Separated by Three Commercial Kits for Prostate Cancer Diagnosis. International Journal of Nanomedicine 15, pages 10241-10256.
Read now
Marije E. Kuipers, Esther N.M. Nolte-‘t Hoen, Alwin J. van der Ham, Arifa Ozir-Fazalalikhan, D. Linh Nguyen, Clarize M. de Korne, Roman I. Koning, John J. Tomes, Karl F. Hoffmann, Hermelijn H. Smits & Cornelis H. Hokke. (2020) DC-SIGN mediated internalisation of glycosylated extracellular vesicles from Schistosoma mansoni increases activation of monocyte-derived dendritic cells. Journal of Extracellular Vesicles 9:1.
Read now

Articles from other publishers (63)

Zun Y. Guo, Yue Tang & Yi C. Cheng. (2024) Exosomes as Targeted Delivery Drug System: Advances in Exosome Loading, Surface Functionalization and Potential for Clinical Application. Current Drug Delivery 21:4, pages 473-487.
Crossref
Vijaya Sunkara, Juhee Park, Jiyun Han, Jonathan Sabaté del Río, Hyun-Ju Cho, In-Jae Oh & Yoon-Kyoung Cho. (2023) Exosome Precipitation by Ionic Strength Modulation: ExoPRISM. ACS Applied Materials & Interfaces.
Crossref
Chunman Huang, Liwei Li, Yongbin Huang, Jingqun Pang & Guanghua Chen. (2023) Advances in targeted modification of extracellular vesicles. Chinese Science Bulletin 68:33, pages 4532-4543.
Crossref
Andrea Ridolfi, Laura Conti, Marco Brucale, Roberto Frigerio, Jacopo Cardellini, Angelo Musicò, Miriam Romano, Andrea Zendrini, Laura Polito, Greta Bergamaschi, Alessandro Gori, Costanza Montis, Stefano Panella, Lucio Barile, Debora Berti, Annalisa Radeghieri, Paolo Bergese, Marina Cretich & Francesco Valle. (2023) Particle profiling of EV‐lipoprotein mixtures by AFM nanomechanical imaging. Journal of Extracellular Vesicles 12:10.
Crossref
Md. Khirul Islam, Misba Khan, Kamlesh Gidwani, Kenneth W. Witwer, Urpo Lamminmäki & Janne Leivo. (2023) Lectins as potential tools for cancer biomarker discovery from extracellular vesicles. Biomarker Research 11:1.
Crossref
Will Thompson & Eleftherios Terry Papoutsakis. (2023) The role of biomechanical stress in extracellular vesicle formation, composition and activity. Biotechnology Advances 66, pages 108158.
Crossref
Yijie Chen, Yonglei Wu, Jiaomei Li, Chunhui Deng & Nianrong Sun. (2023) Resol/triblock copolymer composite-guided smart fabrication of carbonized mesopores for efficiently decoding exosomal glycans. Microchimica Acta 190:8.
Crossref
Yang Li, Jun Wang, Weiyu Chen, Haojie Lu & Ying Zhang. (2023) Comprehensive review of MS‐based studies on N‐glycoproteome and N‐glycome of extracellular vesicles. PROTEOMICS.
Crossref
Qi Li, Huilei Yu, Fengyuan Zhao, Chenxi Cao, Tong Wu, Yifei Fan, Yingfang Ao & Xiaoqing Hu. (2023) 3D Printing of Microenvironment‐Specific Bioinspired and Exosome‐Reinforced Hydrogel Scaffolds for Efficient Cartilage and Subchondral Bone Regeneration. Advanced Science.
Crossref
Álvaro M. Martins, Tânia M. Lopes, Francisca Diniz, José Pires, Hugo Osório, Filipe Pinto, Daniela Freitas & Celso A. Reis. (2023) Differential Protein and Glycan Packaging into Extracellular Vesicles in Response to 3D Gastric Cancer Cellular Organization. Advanced Science.
Crossref
Valentina Bettio, Eleonora Mazzucco, Annamaria Antona, Silvia Cracas, Marco Varalda, Jacopo Venetucci, Stefania Bruno, Giulia Chiabotto, Chiara Venegoni, Alessandra Vasile, Annalisa Chiocchetti, Marco Quaglia, Giovanni Camussi, Vincenzo Cantaluppi, Massimiliano Panella, Roberta Rolla, Marcello Manfredi & Daniela Capello. (2023) Extracellular vesicles from human plasma for biomarkers discovery: Impact of anticoagulants and isolation techniques. PLOS ONE 18:5, pages e0285440.
Crossref
Shu Zhu, Yaohui Fang, Kefan Guo, Zhonghua Ni & Nan Xiang. (2023) Next‐generation liquid biopsy instruments: Challenges and opportunities. ELECTROPHORESIS 44:9-10, pages 775-783.
Crossref
Stephanie M. Kronstadt, Lauren Hoorens Van Heyningen, Amaya Aranda & Steven M. Jay. (2023) Assessment of anti-inflammatory bioactivity of extracellular vesicles is susceptible to error via media component contamination. Cytotherapy 25:4, pages 387-396.
Crossref
Asako Shimoda & Kazunari Akiyoshi. (2023) Surface Glycan Profiling of Extracellular Vesicles by Lectin Microarray and Glycoengineering for Control of Cellular Interactions. Pharmaceutical Research 40:4, pages 795-800.
Crossref
Fangxu Sun, Suttipong Suttapitugsakul & Ronghu Wu. (2021) Systematic characterization of extracellular glycoproteins using mass spectrometry. Mass Spectrometry Reviews 42:2, pages 519-545.
Crossref
Gabriella Dobra, Edina Gyukity-Sebestyén, Mátyás Bukva, Mária Harmati, Valentina Nagy, Zoltán Szabó, Tibor Pankotai, Álmos Klekner & Krisztina Buzás. (2023) MMP-9 as Prognostic Marker for Brain Tumours: A Comparative Study on Serum-Derived Small Extracellular Vesicles. Cancers 15:3, pages 712.
Crossref
Jenifer P. Goncalves, Vatsal J. Deliwala, Daniel Kolarich, Fernando Souza-Fonseca-Guimaraes & Joy Wolfram. (2022) The cancer cell-derived extracellular vesicle glycocode in immunoevasion. Trends in Immunology 43:11, pages 864-867.
Crossref
Szeliski K, Drewa T & Pokrywczyńska M. (2022) Small extracellular vesicles as a multicomponent biomarker platform in urinary tract carcinomas. Frontiers in Molecular Biosciences 9.
Crossref
Marta Clos-Sansalvador, Sergio G. Garcia, Miriam Morón-Font, Charles Williams, Niels-Christian Reichardt, Juan M. Falcón-Pérez, Antoni Bayes-Genis, Santiago Roura, Marcella Franquesa, Marta Monguió-Tortajada & Francesc E. Borràs. (2022) N-Glycans in Immortalized Mesenchymal Stromal Cell-Derived Extracellular Vesicles Are Critical for EV–Cell Interaction and Functional Activation of Endothelial Cells. International Journal of Molecular Sciences 23:17, pages 9539.
Crossref
Veronika Kralj-Iglič, Gabriella Pocsfalvi & Aleš Iglič. 2022. Extracellular Vesicles - Role in Diseases, Pathogenesis and Therapy. Extracellular Vesicles - Role in Diseases, Pathogenesis and Therapy.
Jaroslav Juracek, Marie Madrzyk, Michal Stanik & Ondrej Slaby. (2022) Urinary microRNAs and Their Significance in Prostate Cancer Diagnosis: A 5-Year Update. Cancers 14:13, pages 3157.
Crossref
Nada Basheir Ali, Ahmad Faizal Abdull Razis, Der Jiun Ooi, Kim Wei Chan, Norsharina Ismail & Jhi Biau Foo. (2022) Theragnostic Applications of Mammal and Plant-Derived Extracellular Vesicles: Latest Findings, Current Technologies, and Prospects. Molecules 27:12, pages 3941.
Crossref
Marco Pirisinu, Tin Chanh Pham, Daniel Xin Zhang, Tran Nguyen Hong, Lap Thi Nguyen & Minh TN Le. (2022) Extracellular vesicles as natural therapeutic agents and innate drug delivery systems for cancer treatment: Recent advances, current obstacles, and challenges for clinical translation. Seminars in Cancer Biology 80, pages 340-355.
Crossref
Valeria da Costa & Teresa Freire. (2022) Advances in the Immunomodulatory Properties of Glycoantigens in Cancer. Cancers 14:8, pages 1854.
Crossref
Yanqiu Hua, Xiulin Chang, Liaoqiong FangZhibiao Wang. (2022) Subgroups of Extracellular Vesicles: Can They Be Defined by “Labels?”. DNA and Cell Biology.
Crossref
Keisuke Nagao, Kento Maeda, Kasumi Hosomi, Kaito Morioka, Tatsutoshi Inuzuka & Kazuaki Ohtsubo. (2022) Sialyl-Tn antigen facilitates extracellular vesicle-mediated transfer of FAK and enhances motility of recipient cells. The Journal of Biochemistry.
Crossref
Renato Massis Souza Campos, Grasielle Pereira Jannuzzi, Marcelo Augusto Kazuo Ikeda, Sandro Rogério de Almeida & Karen Spadari Ferreira. (2021) Extracellular Vesicles From Sporothrix brasiliensis Yeast Cells Increases Fungicidal Activity in Macrophages. Mycopathologia 186:6, pages 807-818.
Crossref
Giusi Alberti, Christian M. Sánchez-López, Alexia Andres, Radha Santonocito, Claudia Campanella, Francesco Cappello & Antonio Marcilla. (2021) Molecular Profile Study of Extracellular Vesicles for the Identification of Useful Small “Hit” in Cancer Diagnosis. Applied Sciences 11:22, pages 10787.
Crossref
Marija Holcar, Maša Kandušer & Metka Lenassi. (2021) Blood Nanoparticles – Influence on Extracellular Vesicle Isolation and Characterization. Frontiers in Pharmacology 12.
Crossref
Vincenzo Verdi, Anaïs Bécot, Guillaume Niel & Frederik J. Verweij. (2021) In vivo imaging of EVs in zebrafish: New perspectives from “the waterside”. FASEB BioAdvances 3:11, pages 918-929.
Crossref
Liang Wu, Karin Boer, Wouter W. Woud, Suwasin Udomkarnjananun, Dennis A. Hesselink & Carla C. Baan. (2021) Urinary Extracellular Vesicles Are a Novel Tool to Monitor Allograft Function in Kidney Transplantation: A Systematic Review. International Journal of Molecular Sciences 22:19, pages 10499.
Crossref
Rodolphe Poupardin, Martin Wolf & Dirk Strunk. (2021) Adherence to minimal experimental requirements for defining extracellular vesicles and their functions. Advanced Drug Delivery Reviews 176, pages 113872.
Crossref
Kaylan K. Jackson, Rhonda R. Powell, Terri F. Bruce & R. Kenneth Marcus. (2021) Rapid isolation of extracellular vesicles from diverse biofluid matrices via capillary-channeled polymer fiber solid-phase extraction micropipette tips . The Analyst 146:13, pages 4314-4325.
Crossref
Cherrie D. Sherman, Shweta Lodha & Susmita Sahoo. (2021) EV Cargo Sorting in Therapeutic Development for Cardiovascular Disease. Cells 10:6, pages 1500.
Crossref
Ana Magalhães, Henrique O. Duarte & Celso A. Reis. (2021) The role of O-glycosylation in human disease. Molecular Aspects of Medicine 79, pages 100964.
Crossref
Uta Erdbrügger, Charles J. Blijdorp, Irene V. Bijnsdorp, Francesc E. Borràs, Dylan Burger, Benedetta Bussolati, James Brian Byrd, Aled Clayton, James W. Dear, Juan M. Falcón‐Pérez, Cristina Grange, Andrew F. Hill, Harry Holthöfer, Ewout J. Hoorn, Guido Jenster, Connie R. Jimenez, Kerstin Junker, John Klein, Mark A. Knepper, Erik H. Koritzinsky, James M. Luther, Metka Lenassi, Janne Leivo, Inge Mertens, Luca Musante, Eline Oeyen, Maija Puhka, Martin E. Royen, Catherine Sánchez, Carolina Soekmadji, Visith Thongboonkerd, Volkert Steijn, Gerald Verhaegh, Jason P. Webber, Kenneth Witwer, Peter S.T. Yuen, Lei Zheng, Alicia Llorente & Elena S. Martens‐Uzunova. (2021) Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles. Journal of Extracellular Vesicles 10:7.
Crossref
Cristiano Carlomagno, Chiara Giannasi, Stefania Niada, Marzia Bedoni, Alice Gualerzi & Anna Teresa Brini. (2021) Raman Fingerprint of Extracellular Vesicles and Conditioned Media for the Reproducibility Assessment of Cell-Free Therapeutics. Frontiers in Bioengineering and Biotechnology 9.
Crossref
Qi Li, Huilei Yu, Muyang Sun, Peng Yang, Xiaoqing Hu, Yingfang Ao & Jin Cheng. (2021) The tissue origin effect of extracellular vesicles on cartilage and bone regeneration. Acta Biomaterialia 125, pages 253-266.
Crossref
Mengrou Lu, Emma DiBernardo, Emily Parks, Hannah Fox, Si-Yang Zheng & Elizabeth Wayne. (2021) The Role of Extracellular Vesicles in the Pathogenesis and Treatment of Autoimmune Disorders. Frontiers in Immunology 12.
Crossref
Sören Kuypers, Nick Smisdom, Isabel Pintelon, Jean‐Pierre Timmermans, Marcel Ameloot, Luc Michiels, Jelle Hendrix & Baharak Hosseinkhani. (2021) Unsupervised Machine Learning‐Based Clustering of Nanosized Fluorescent Extracellular Vesicles. Small 17:5, pages 2006786.
Crossref
Carmen Almiñana, Alba Rudolf Vegas, Muhittin Tekin, Mubbashar Hassan, Rustem Uzbekov, Thomas Fröhlich, Heinrich Bollwein & Stefan Bauersachs. (2021) Isolation and Characterization of Equine Uterine Extracellular Vesicles: A Comparative Methodological Study. International Journal of Molecular Sciences 22:2, pages 979.
Crossref
Álvaro M. Martins, Cátia C. Ramos, Daniela Freitas & Celso A. Reis. (2021) Glycosylation of Cancer Extracellular Vesicles: Capture Strategies, Functional Roles and Potential Clinical Applications. Cells 10:1, pages 109.
Crossref
Fei Xue, Yundi Chen, Yi Wen, Komal Abhange, Wenlong Zhang, Gong Cheng, Zachary Quinn, Wenjun Mao & Yuan Wan. (2021) Isolation of extracellular vesicles with multivalent aptamers. The Analyst 146:1, pages 253-261.
Crossref
Andreia Peixoto, Sofia Cotton, Lúcio Lara Santos & José Alexandre Ferreira. 2021. Tumor Microenvironment. Tumor Microenvironment 1 33 .
Brandon M. Lehrich, Yaxuan Liang & Massimo S. Fiandaca. (2021) Foetal bovine serum influence on in vitro extracellular vesicle analyses. Journal of Extracellular Vesicles 10:3.
Crossref
Veronika Kralj-Iglič, Gabriella Pocsfalvi, Luka Mesarec, Vid Šuštar, Henry Hägerstrand & Aleš Iglič. (2020) Minimizing isotropic and deviatoric membrane energy – An unifying formation mechanism of different cellular membrane nanovesicle types. PLOS ONE 15:12, pages e0244796.
Crossref
Laura Le Gall, Zamalou Gisele Ouandaogo, Ekene Anakor, Owen Connolly, Gillian Butler Browne, Jeanne Laine, William Duddy & Stephanie Duguez. (2020) Optimized method for extraction of exosomes from human primary muscle cells. Skeletal Muscle 10:1.
Crossref
Yanlong Xing, Ziyi Cheng, Rui Wang, Chuanzhu Lv, Tony D. James & Fabiao Yu. (2020) Analysis of extracellular vesicles as emerging theranostic nanoplatforms. Coordination Chemistry Reviews 424, pages 213506.
Crossref
Federica Anastasi, Francesco Greco, Marialaura Dilillo, Eleonora Vannini, Valentina Cappello, Laura Baroncelli, Mario Costa, Mauro Gemmi, Matteo Caleo & Liam A. McDonnell. (2020) Proteomics analysis of serum small extracellular vesicles for the longitudinal study of a glioblastoma multiforme mouse model. Scientific Reports 10:1.
Crossref
Jing Du, Chao Yuan, Weijie Wang, Zitong Yu, Rui Hao, Yi Zhang, Min Guan, Nan Li & Hui Yang. (2020) Aptasensor-enabled quantitative analysis of nano-sized extracellular vesicles by flow cytometry. The Analyst 145:23, pages 7551-7558.
Crossref
Yaoyun Kuang, Xuan Zheng, Lin Zhang, Xiaoyu Ai, Vivek Venkataramani, Ertugrul Kilic, Dirk M. Hermann, Arshad Majid, Mathias Bähr & Thorsten R. Doeppner. (2020) Adipose‐derived mesenchymal stem cells reduce autophagy in stroke mice by extracellular vesicle transfer of miR‐25. Journal of Extracellular Vesicles 10:1.
Crossref
Daniel S. K. Liu, Flora M. Upton, Eleanor Rees, Christopher Limb, Long R. Jiao, Jonathan Krell & Adam E. Frampton. (2020) Size-Exclusion Chromatography as a Technique for the Investigation of Novel Extracellular Vesicles in Cancer. Cancers 12:11, pages 3156.
Crossref
Junli Zhang, Jinjin Shi, Hongling Zhang, Yifan Zhu, Wei Liu, Kaixiang Zhang & Zhenzhong Zhang. (2020) Localized fluorescent imaging of multiple proteins on individual extracellular vesicles using rolling circle amplification for cancer diagnosis. Journal of Extracellular Vesicles 10:1.
Crossref
Shanyi Lin, Shumin Zhou & Ting Yuan. (2020) The “sugar‐coated bullets” of cancer: Tumor‐derived exosome surface glycosylation from basic knowledge to applications. Clinical and Translational Medicine 10:6.
Crossref
Xuan T. T. Dang, Jayasinghe Migara Kavishka, Daniel Xin Zhang, Marco Pirisinu & Minh T. N. Le. (2020) Extracellular Vesicles as an Efficient and Versatile System for Drug Delivery. Cells 9:10, pages 2191.
Crossref
Anna Arola-Arnal, María-Carmen López de las Hazas, Lisard Iglesias-Carres, Diana C. Mantilla-Escalante, Manuel Suárez, Rebeca Busto, Francesco Visioli, Cinta Bladé & Alberto Dávalos. (2020) Exosomes transport trace amounts of (poly)phenols. Food & Function 11:9, pages 7784-7792.
Crossref
Manu Sharma, Pedro Morgado, Hanbang Zhang, Gretchen Ehrenkaufer, Dipak Manna & Upinder Singh. (2020) Characterization of Extracellular Vesicles from Entamoeba histolytica Identifies Roles in Intercellular Communication That Regulates Parasite Growth and Development. Infection and Immunity 88:10.
Crossref
Ketki Holkar, Anuradha Vaidya, Prasad Pethe, Vaijayanti Kale & Ganesh Ingavle. (2020) Biomaterials and extracellular vesicles in cell-free therapy for bone repair and regeneration: Future line of treatment in regenerative medicine. Materialia 12, pages 100736.
Crossref
Shuangdan Zhao, Wenbo Qi, Jiahua Zheng, Yanpeng Tian, Xuejun Qi, Desheng Kong, Jingkun Zhang & Xianghua Huang. (2020) Exosomes Derived from Adipose Mesenchymal Stem Cells Restore Functional Endometrium in a Rat Model of Intrauterine Adhesions. Reproductive Sciences 27:6, pages 1266-1275.
Crossref
Maria Antònia Forteza-Genestra, Miquel Antich-Rosselló, Javier Calvo, Antoni Gayà, Marta Monjo & Joana Maria Ramis. (2020) Purity Determines the Effect of Extracellular Vesicles Derived from Mesenchymal Stromal Cells. Cells 9:2, pages 422.
Crossref
Suttipong Suttapitugsakul, Fangxu Sun & Ronghu Wu. (2019) Recent Advances in Glycoproteomic Analysis by Mass Spectrometry. Analytical Chemistry 92:1, pages 267-291.
Crossref
Darja Božič, Matej Hočevar, Veno Kononenko, Marko Jeran, Urška Štibler, Immacolata Fiume, Manca Pajnič, Ljubiša Pađen, Ksenija Kogej, Damjana Drobne, Aleš Iglič, Gabriella Pocsfalvi & Veronika Kralj-Iglič. 2020. 113 155 .
Charles Williams, Mari Palviainen, Niels-Christian Reichardt, Pia R.-M. Siljander & Juan M. Falcón-Pérez. (2019) Metabolomics Applied to the Study of Extracellular Vesicles. Metabolites 9:11, pages 276.
Crossref