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Research article

A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity

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Yiming Zhang, Yiming Dou, Yang Liu, Mingyuan Di, Hanming Bian, Xun Sun & Qiang Yang. (2023) Advances in Therapeutic Applications of Extracellular Vesicles. International Journal of Nanomedicine 18, pages 3285-3307.
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Jian Yang, Xin Gao, Xiaotao Xing, Haisen Huang, Qi Tang, Shixing Ma, Xun Xu, Cheng Liang, Maojiao Li, Li Liao & Weidong Tian. (2021) An Isolation System to Collect High Quality and Purity Extracellular Vesicles from Serum. International Journal of Nanomedicine 16, pages 6681-6692.
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Daria Kozhevnikova, Vasiliy Chernyshev & Alexey Yashchenok. (2023) Progress in Isolation and Molecular Profiling of Small Extracellular Vesicles via Bead-Assisted Platforms. Biosensors 13:7, pages 688.
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Qin Zhang, Dennis K. Jeppesen, James N. Higginbotham, Jeffrey L. Franklin & Robert J. Coffey. (2023) Comprehensive isolation of extracellular vesicles and nanoparticles. Nature Protocols 18:5, pages 1462-1487.
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Xue Yao, Baixue Liao, Feng Chen, Lüye Liu, Kaiwen Wu, Yaying Hao, Yanping Li, Yuebin Wang, Ruiling Fan, Jun Yin, Lei Liu & Yuanbiao Guo. (2023) Comparison of proteomic landscape of extracellular vesicles in pleural effusions isolated by three strategies. Frontiers in Bioengineering and Biotechnology 11.
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Crossref
Terjahna Richards, Himaxi Patel, Ketan Patel & Frank Schanne. (2023) Endogenous Lipid Carriers—Bench-to-Bedside Roadblocks in Production and Drug Loading of Exosomes. Pharmaceuticals 16:3, pages 421.
Crossref
Tânia Soares Martins, Margarida Vaz & Ana Gabriela Henriques. (2022) A review on comparative studies addressing exosome isolation methods from body fluids. Analytical and Bioanalytical Chemistry 415:7, pages 1239-1263.
Crossref
Mar Casajuana Ester & Richard M. Day. (2023) Production and Utility of Extracellular Vesicles with 3D Culture Methods. Pharmaceutics 15:2, pages 663.
Crossref
Yujin Lee, Jie Ni, Julia Beretov, Valerie C. Wasinger, Peter Graham & Yong Li. (2023) Recent advances of small extracellular vesicle biomarkers in breast cancer diagnosis and prognosis. Molecular Cancer 22:1.
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Krizia Sagini, Lorena Urbanelli, Sandra Buratta, Carla Emiliani & Alicia Llorente. (2023) Lipid Biomarkers in Liquid Biopsies: Novel Opportunities for Cancer Diagnosis. Pharmaceutics 15:2, pages 437.
Crossref
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Crossref
O.G. Davies, S. Williams & K. Goldie. (2023) The therapeutic and commercial landscape of stem cell vesicles in regenerative dermatology. Journal of Controlled Release 353, pages 1096-1106.
Crossref
Naomi Buntsma, Edwin van der Pol, Rienk Nieuwland & Aleksandra Gąsecka. 2023. Extracellular Vesicles in Cardiovascular and Metabolic Diseases. Extracellular Vesicles in Cardiovascular and Metabolic Diseases 81 103 .
Quentin Roux, Sarah Deville & An Hendrix. 2023. Mass Spectrometry-Based Proteomics. Mass Spectrometry-Based Proteomics 253 269 .
Karina P. De Sousa, Izadora Rossi, Mahamed Abdullahi, Marcel Ivan Ramirez, Dan Stratton & Jameel Malhador Inal. (2022) Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy. WIREs Nanomedicine and Nanobiotechnology 15:1.
Crossref
Qian Wu, Siyuan Fu, Hanyang Xiao, Jiaxin Du, Fang Cheng, Shuangshuang Wan, Houjuan Zhu, Dan Li, Fei Peng, Xianguang Ding & Lianhui Wang. (2022) Advances in Extracellular Vesicle Nanotechnology for Precision Theranostics. Advanced Science 10:3.
Crossref
Margarida Vaz, Tânia Soares Martins & Ana Gabriela Henriques. (2022) Extracellular vesicles in the study of Alzheimer's and Parkinson's diseases: Methodologies applied from cells to biofluids. Journal of Neurochemistry 163:4, pages 266-309.
Crossref
Annika Pfeiffer, Jennifer D. Petersen, Guido H. Falduto, David Eric Anderson, Joshua Zimmerberg, Dean D. Metcalfe & Ana Olivera. (2022) Selective immunocapture reveals neoplastic human mast cells secrete distinct microvesicle‐ and exosome‐like populations of KIT‐containing extracellular vesicles. Journal of Extracellular Vesicles 11:10.
Crossref
María Cristina Negrete-García, Javier de Jesús Ramos-Abundis, Noé Alvarado-Vasquez, Eduardo Montes-Martínez, Martha Montaño, Carlos Ramos & Bettina Sommer. (2022) Exosomal Micro-RNAs as Intercellular Communicators in Idiopathic Pulmonary Fibrosis. International Journal of Molecular Sciences 23:19, pages 11047.
Crossref
Laura Benecke, Dapi Chiang, Eliane Ebnoether, Michael Pfaffl & Laurent Muller. (2022) Isolation and analysis of tumor‑derived extracellular vesicles from head and neck squamous cell carcinoma plasma by galectin‑based glycan recognition particles. International Journal of Oncology 61:5.
Crossref
Xia Wang, Lu Tian, Jingyi Lu & Irene Oi-Lin Ng. (2022) Exosomes and cancer - Diagnostic and prognostic biomarkers and therapeutic vehicle. Oncogenesis 11:1.
Crossref
Pauline Po Yee Lui & Yung Tim Leung. (2022) Practical Considerations for Translating Mesenchymal Stromal Cell-Derived Extracellular Vesicles from Bench to Bed. Pharmaceutics 14:8, pages 1684.
Crossref
Diogo Fortunato, Stavros Giannoukakos, Ana Giménez-Capitán, Michael Hackenberg, Miguel A. Molina-Vila & Nataša Zarovni. (2022) Selective isolation of extracellular vesicles from minimally processed human plasma as a translational strategy for liquid biopsies. Biomarker Research 10:1.
Crossref
Vasiliy S. Chernyshev, Roman N. Chuprov‐Netochin, Ekaterina Tsydenzhapova, Elena V. Svirshchevskaya, Rimma A. Poltavtseva, Anastasiia Merdalimova, Alexey Yashchenok, Amiran Keshelava, Konstantin Sorokin, Varlam Keshelava, Gennadiy T. Sukhikh, Dmitry Gorin, Sergey Leonov & Mikhail Skliar. (2022) Asymmetric depth‐filtration: A versatile and scalable method for high‐yield isolation of extracellular vesicles with low contamination. Journal of Extracellular Vesicles 11:8.
Crossref
Yohan Kim, Edwin van der Pol, Ali Arafa, Ishwor Thapa, Cameron J. Britton, Jorgena Kosti, Siyang Song, Vidhu B. Joshi, Ree M. Erickson, Hesham Ali & Fabrice Lucien. (2022) Calibration and standardization of extracellular vesicle measurements by flow cytometry for translational prostate cancer research. Nanoscale 14:27, pages 9781-9795.
Crossref
Rienk Nieuwland, Pia R.-M. Siljander, Juan M. Falcón-Pérez & Kenneth W. Witwer. (2022) Reproducibility of extracellular vesicle research. European Journal of Cell Biology 101:3, pages 151226.
Crossref
Vasiliy S. Chernyshev, Roman N. Chuprov-Netochin, Ekaterina Tsydenzhapova, Brian Van Devener, Sergey Leonov, Dmitry Gorin & Mikhail Skliar. (2022) Dynamic surface tension probe for measuring the concentration of extracellular vesicles. Biochemical and Biophysical Research Communications 609, pages 189-194.
Crossref
André Görgens, Giulia Corso, Daniel W. Hagey, Rim Jawad Wiklander, Manuela O. Gustafsson, Ulrika Felldin, Yi Lee, R. Beklem Bostancioglu, Helena Sork, Xiuming Liang, Wenyi Zheng, Dara K. Mohammad, Simonides I. van de Wakker, Pieter Vader, Antje M. Zickler, Doste R. Mamand, Li Ma, Margaret N. Holme, Molly M. Stevens, Oscar P. B. Wiklander & Samir EL Andaloussi. (2022) Identification of storage conditions stabilizing extracellular vesicles preparations. Journal of Extracellular Vesicles 11:6.
Crossref
Sarah Beck, Bernhard Hochreiter & Johannes A. Schmid. (2022) Extracellular Vesicles Linking Inflammation, Cancer and Thrombotic Risks. Frontiers in Cell and Developmental Biology 10.
Crossref
Andrea L. Estrada, Zackary J. Valenti, Gabriella Hehn, Adam J. Amorese, Nicholas S. Williams, Nicholas P. Balestrieri, Clayton Deighan, Christopher P. Allen, Espen E. Spangenburg, Nicole A. Kruh-Garcia & Daniel S. Lark. (2022) Extracellular vesicle secretion is tissue-dependent ex vivo and skeletal muscle myofiber extracellular vesicles reach the circulation in vivo. American Journal of Physiology-Cell Physiology 322:2, pages C246-C259.
Crossref
Haoyang Zheng, Jiandong Zhao, Xuantang Wang, Shaohan Yan, Huimin Chu, Mingxia Gao & Xiangmin Zhang. (2022) Integrated Pipeline of Rapid Isolation and Analysis of Human Plasma Exosomes for Cancer Discrimination Based on Deep Learning of MALDI-TOF MS Fingerprints. Analytical Chemistry 94:3, pages 1831-1839.
Crossref
Niamh McNamee, Róisín Daly, John Crown & Lorraine O'Driscoll. (2022) A method of separating extracellular vesicles from blood shows potential clinical translation, and reveals extracellular vesicle cargo gremlin-1 as a diagnostic biomarker. Translational Oncology 15:1, pages 101274.
Crossref
S.I. van de Wakker, J. van Oudheusden, E.A. Mol, M.T. Roefs, W. Zheng, A. Görgens, S. El Andaloussi, J.P.G. Sluijter & P. Vader. (2022) Influence of short term storage conditions, concentration methods and excipients on extracellular vesicle recovery and function. European Journal of Pharmaceutics and Biopharmaceutics 170, pages 59-69.
Crossref
Vincent Hyenne, Jacky G. Goetz & Naël Osmani. 2022. Microfluidics and Biosensors in Cancer Research. Microfluidics and Biosensors in Cancer Research 341 368 .
Kaloyan Takov, I-Jou Teng & Manuel Mayr. 2022. Extracellular Vesicles in Diagnosis and Therapy. Extracellular Vesicles in Diagnosis and Therapy 31 40 .
Eric W. Deutsch, Gilbert S. Omenn, Zhi Sun, Michal Maes, Maria Pernemalm, Krishnan K. Palaniappan, Natasha Letunica, Yves Vandenbrouck, Virginie Brun, Sheng-ce Tao, Xiaobo Yu, Philipp E. Geyer, Vera Ignjatovic, Robert L. Moritz & Jochen M. Schwenk. (2021) Advances and Utility of the Human Plasma Proteome. Journal of Proteome Research 20:12, pages 5241-5263.
Crossref
Qingfu Zhu, Yijiang Huang, Qinsi Yang & Fei Liu. (2021) Recent technical advances to study metabolomics of extracellular vesicles. Microchemical Journal 171, pages 106816.
Crossref
Dmitry Ter-Ovanesyan, Maia Norman, Roey Lazarovits, Wendy Trieu, Ju-Hyun Lee, George M Church & David R Walt. (2021) Framework for rapid comparison of extracellular vesicle isolation methods. eLife 10.
Crossref
Marija Holcar, Maša Kandušer & Metka Lenassi. (2021) Blood Nanoparticles – Influence on Extracellular Vesicle Isolation and Characterization. Frontiers in Pharmacology 12.
Crossref
Isabelle Grenier-Pleau & Sheela A. Abraham. (2021) Extracellular vesicles tell all: How vesicle-mediated cellular communication shapes hematopoietic stem cell biology with increasing age. Experimental Hematology 101-102, pages 7-15.
Crossref
Glenn Vergauwen, Joeri Tulkens, Cláudio Pinheiro, Francisco Avila Cobos, Sándor Dedeyne, Marie‐Angélique De Scheerder, Linos Vandekerckhove, Francis Impens, Ilkka Miinalainen, Geert Braems, Kris Gevaert, Pieter Mestdagh, Jo Vandesompele, Hannelore Denys, Olivier De Wever & An Hendrix. (2021) Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions. Journal of Extracellular Vesicles 10:10.
Crossref
Dominik Buschmann, Veronika Mussack & James Brian Byrd. (2021) Separation, characterization, and standardization of extracellular vesicles for drug delivery applications. Advanced Drug Delivery Reviews 174, pages 348-368.
Crossref
Rosanne E. Veerman, Loes Teeuwen, Paulo Czarnewski, Gözde Güclüler Akpinar, AnnSofi Sandberg, Xiaofang Cao, Maria Pernemalm, Lukas M. Orre, Susanne Gabrielsson & Maria Eldh. (2021) Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin. Journal of Extracellular Vesicles 10:9.
Crossref
Mei Xue, Wei Chen & Xu Li. (2021) Extracellular vesicle-transferred long noncoding RNAs in bladder cancer. Clinica Chimica Acta 516, pages 34-45.
Crossref
Roberto Frigerio, Angelo Musicò, Marco Brucale, Andrea Ridolfi, Silvia Galbiati, Riccardo Vago, Greta Bergamaschi, Anna Maria Ferretti, Marcella Chiari, Francesco Valle, Alessandro Gori & Marina Cretich. (2021) Extracellular Vesicles Analysis in the COVID-19 Era: Insights on Serum Inactivation Protocols towards Downstream Isolation and Analysis. Cells 10:3, pages 544.
Crossref
Sreerenjini Lakshmi, Thomas A. Hughes & Sulochana Priya. (2021) Exosomes and exosomal RNAs in breast cancer: A status update. European Journal of Cancer 144, pages 252-268.
Crossref
Brandon M. Lehrich, Yaxuan Liang & Massimo S. Fiandaca. (2021) Foetal bovine serum influence on in vitro extracellular vesicle analyses. Journal of Extracellular Vesicles 10:3.
Crossref
Nea Bister, Cristiana Pistono, Benjamin Huremagic, Jukka Jolkkonen, Rosalba Giugno & Tarja Malm. (2020) Hypoxia and extracellular vesicles: A review on methods, vesicular cargo and functions. Journal of Extracellular Vesicles 10:1.
Crossref
Xiaogang Zhang, Harmjan R. Vos, Weiyang Tao & Willem Stoorvogel. (2020) Proteomic Profiling of Two Distinct Populations of Extracellular Vesicles Isolated from Human Seminal Plasma. International Journal of Molecular Sciences 21:21, pages 7957.
Crossref
Karim Sidhom, Patience O. Obi & Ayesha Saleem. (2020) A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option?. International Journal of Molecular Sciences 21:18, pages 6466.
Crossref