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Journal of Liquid Chromatography & Related Technologies Volume 23, 2000 - Issue 4
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Original Articles

PURIFICATION OF PEG-PROTEIN CONJUGATES BY COUNTERCURRENT DISTRIBUTION IN AQUEOUS TWO-PHASE SYSTEMS

Terasut Sookkumnerd Biopharmaceutical Technology Institute, Department of Chemical Engineering, Lehigh University , Bethlehem, PA, 18015, U.S.A.
&
James T. Hsu Biopharmaceutical Technology Institute, Department of Chemical Engineering, Lehigh University , Bethlehem, PA, 18015, U.S.A.
Pages 497-503 | Received 07 Jun 1999, Accepted 26 Jun 1999, Published online: 06 Feb 2007

Citations (16)

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Read on this site (2)

José González-Valdez, Marco Rito-Palomares & Jorge Benavides. (2011) Quantification of RNase A and Its PEGylated Conjugates on Polymer-Salt Rich Environments Using UV Spectrophotometry. Analytical Letters 44:5, pages 800-814.
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Terasut Sookkumnerd, James T. Hsu & Yoichiro Ito. (2000) PURIFICATION OF PEG-PROTEIN CONJUGATES BY CENTRIFUGAL PRECIPITATION CHROMATOGRAPHY. Journal of Liquid Chromatography & Related Technologies 23:13, pages 1973-1979.
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Articles from other publishers (14)

João H. P. M. Santos, Carlos M. N. Mendonça, Amanda R. P. Silva, Ricardo P. S. Oliveira, Adalberto Pessoa, João A. P. Coutinho, Sónia P. M. Ventura & Carlota O. Rangel-Yagui. (2019) An integrated process combining the reaction and purification of PEGylated proteins. Green Chemistry 21:23, pages 6407-6418.
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João H. P. M. Santos, Ana M. Ferreira, Mafalda R. Almeida, Paula S. G. N. Quinteiro, Ana C. R. V. Dias, João A. P. Coutinho, Mara G. Freire, Carlota O. Rangel-Yagui & Sónia P. M. Ventura. (2019) Continuous separation of cytochrome-c PEGylated conjugates by fast centrifugal partition chromatography. Green Chemistry 21:20, pages 5501-5506.
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João Henrique Picado Madalena Santos, Karin Mariana Torres-Obreque, Giovanna Pastore Meneguetti, Beatriz Panichi Amaro & Carlota Oliveira Rangel-Yagui. (2018) Protein PEGylation for the design of biobetters: from reaction to purification processes. Brazilian Journal of Pharmaceutical Sciences 54:spe.
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Luis Alberto Mejía-Manzano, Karla Mayolo-Deloisa, Calef Sánchez-Trasviña, José González-Valdez, Mirna González-González & Marco Rito-Palomares. (2017) Recovery of PEGylated and native lysozyme using an in situ aqueous two-phase system directly from the PEGylation reaction . Journal of Chemical Technology & Biotechnology 92:10, pages 2519-2526.
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João H. P. M. Santos, Gustavo Carretero, João A. P. Coutinho, Carlota O. Rangel-Yagui & Sónia P. M. Ventura. (2017) Multistep purification of cytochrome c PEGylated forms using polymer-based aqueous biphasic systems. Green Chemistry 19:24, pages 5800-5808.
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David Pfister & Massimo Morbidelli. (2014) Process for protein PEGylation. Journal of Controlled Release 180, pages 134-149.
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José González‐Valdez, Marco Rito‐Palomares & Jorge Benavides. (2013) Effects of chemical modifications in the partition behavior of proteins in aqueous two‐phase systems: A case study with RNase A . Biotechnology Progress 29:2, pages 378-385.
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Miriam Galindo-López & Marco Rito-Palomares. (2013) Practical non-chromatography strategies for the potential separation of PEGylated RNase A conjugates. Journal of Chemical Technology & Biotechnology 88:1, pages 49-54.
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Conan J. Fee & Vinod B. Damodaran. 2012. Biopharmaceutical Production Technology. Biopharmaceutical Production Technology 199 222 .
José González-Valdez, Marco Rito-Palomares & Jorge Benavides. (2012) Advances and trends in the design, analysis, and characterization of polymer–protein conjugates for “PEGylaided” bioprocesses. Analytical and Bioanalytical Chemistry 403:8, pages 2225-2235.
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José González-Valdez, Luisa F. Cueto, Jorge Benavides & Marco Rito-Palomares. (2011) Potential application of aqueous two-phase systems for the fractionation of RNase A and α-Lactalbumin from their PEGylated conjugates. Journal of Chemical Technology & Biotechnology 86:1, pages 26-33.
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Karla Mayolo-Deloisa, José González-Valdez, Daniel Guajardo-Flores, Oscar Aguilar, Jorge Benavides & Marco Rito-Palomares. (2011) Current advances in the non-chromatographic fractionation and characterization of PEGylated proteins. Journal of Chemical Technology & Biotechnology 86:1, pages 18-25.
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Ileana Hernandez-Mireles, Jorge Benavides & Marco Rito-Palomares. (2008) Practical approach to protein recovery by countercurrent distribution in aqueous two-phase systems. Journal of Chemical Technology & Biotechnology 83:2, pages 163-166.
Crossref
M. Schroeder, S. Heumann, C. J. S. M. Silva, A. Cavaco-Paulo & G. M. Guebitz. (2006) Specificities of a chemically modified laccase from Trametes hirsuta on soluble and cellulose-bound substrates. Biotechnology Letters 28:10, pages 741-747.
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