Advanced search
Publication Cover
Molecular and Cellular Biology Volume 15, 1995 - Issue 10
Submit an article Journal homepage
2
Views
81
CrossRef citations to date
0
Altmetric
Research Article

scid Cells Are Deficient in Ku and Replication Protein A Phosphorylation by the DNA-Dependent Protein Kinase

Nikolai V. BoubnovDivision of Tumor Immunology, Dana-Farber Cancer Institute, and Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts02115
&
David T. WeaverDivision of Tumor Immunology, Dana-Farber Cancer Institute, and Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts02115Correspondence[email protected]
Pages 5700-5706 | Received 08 Mar 1995, Accepted 17 Jul 1995, Published online: 30 Mar 2023

Citations (81)

Keep up to date with the latest research on this topic with citation updates for this article.

Subscribe to citation updates

Read on this site (12)

Jinhua Quan & Timur Yusufzai. (2014) HARP preferentially co-purifies with RPA bound to DNA-PK and blocks RPA phosphorylation. Epigenetics 9:5, pages 693-697.
Read now
Hirohiko Yajima, Kyung-Jong Lee & Benjamin P. C. Chen. (2006) ATR-Dependent Phosphorylation of DNA-Dependent Protein Kinase Catalytic Subunit in Response to UV-Induced Replication Stress. Molecular and Cellular Biology 26:20, pages 7520-7528.
Read now
Junsoo Park, Taegun Seo, Hakzoo Kim & Joonho Choe. (2005) Sumoylation of the Novel Protein hRIPβ Is Involved in Replication Protein A Deposition in PML Nuclear Bodies. Molecular and Cellular Biology 25:18, pages 8202-8214.
Read now
Gregory Rodrigo, Sophie Roumagnac, Marc S. Wold, Bernard Salles & Patrick Calsou. (2000) DNA Replication but Not Nucleotide Excision Repair Is Required for UVC-Induced Replication Protein A Phosphorylation in Mammalian Cells. Molecular and Cellular Biology 20:8, pages 2696-2705.
Read now
Ajit Bharti, Stine-Kathrein Kraeft, Mrinal Gounder, Pramod Pandey, Shengfang Jin, Zhi-Min Yuan, Susan P. Lees-Miller, Ralph Weichselbaum, David Weaver, Lan Bo Chen, Donald Kufe & Surender Kharbanda. (1998) Inactivation of DNA-Dependent Protein Kinase by Protein Kinase Cδ: Implications for Apoptosis. Molecular and Cellular Biology 18:11, pages 6719-6728.
Read now
Gilad Mass, Tamar Nethanel & Gabriel Kaufmann. (1998) The Middle Subunit of Replication Protein A Contacts Growing RNA-DNA Primers in Replicating Simian Virus 40 Chromosomes. Molecular and Cellular Biology 18:11, pages 6399-6407.
Read now
Nickolai A. Barlev, Vladimir Poltoratsky, Tom Owen-Hughes, Carol Ying, Lin Liu, Jerry L. Workman & Shelley L. Berger. (1998) Repression of GCN5 Histone Acetyltransferase Activity via Bromodomain-Mediated Binding and Phosphorylation by the Ku–DNA-Dependent Protein Kinase Complex. Molecular and Cellular Biology 18:3, pages 1349-1358.
Read now
Wenhui Li, Patrick Swanson & Stephen Desiderio. (1997) RAG-1 and RAG-2-Dependent Assembly of Functional Complexes with V(D)J Recombination Substrates in Solution. Molecular and Cellular Biology 17:12, pages 6932-6939.
Read now
Sang Eun Lee, Rachel A. Mitchell, Anthony Cheng & Eric A. Hendrickson. (1997) Evidence for DNA-PK-Dependent and -Independent DNA Double-Strand Break Repair Pathways in Mammalian Cells as a Function of the Cell Cycle. Molecular and Cellular Biology 17:3, pages 1425-1433.
Read now
Jayne S. Danska, Dianne P. Holland, Sanjeev Mariathasan, Kelly M. Williams & Cynthia J. Guidos. (1996) Biochemical and Genetic Defects in the DNA-Dependent Protein Kinase in Murine scid Lymphocytes. Molecular and Cellular Biology 16:10, pages 5507-5517.
Read now
Leonard J. Blackwell, James A. Borowiec & Iris A. Mastrangelo. (1996) Single-Stranded-DNA Binding Alters Human Replication Protein A Structure and Facilitates Interaction with DNA-Dependent Protein Kinase. Molecular and Cellular Biology 16:9, pages 4798-4807.
Read now
G. Todd Milne, Shengfang Jin, Katie B. Shannon & David T. Weaver. (1996) Mutations in Two Ku Homologs Define a DNA End-Joining Repair Pathway in Saccharomyces cerevisiae. Molecular and Cellular Biology 16:8, pages 4189-4198.
Read now

Articles from other publishers (69)

Mohammad Saki, Kruttika Bhat, Sirajbir S. Sodhi, Nhan T. Nguyen, Harley I. Kornblum & Frank Pajonk. (2019) Effects of Brain Irradiation in Immune-Competent and Immune-Compromised Mouse Models. Radiation Research 193:2, pages 186.
Crossref
Koji Ando, Ankur K. Shah, Vibhu Sachdev, Benjamin P. Kleinstiver, Julian Taylor-Parker, Moira M. Welch, Yiheng Hu, Ravi Salgia, Forest M. White, Jeffrey D. Parvin, Al Ozonoff, Lucia E. Rameh, J. Keith Joung & Ajit K. Bharti. (2017) Camptothecin resistance is determined by the regulation of topoisomerase I degradation mediated by ubiquitin proteasome pathway. Oncotarget 8:27, pages 43733-43751.
Crossref
Hungjiun Liaw, Deokjae Lee & Kyungjae Myung. (2011) DNA-PK-Dependent RPA2 Hyperphosphorylation Facilitates DNA Repair and Suppresses Sister Chromatid Exchange. PLoS ONE 6:6, pages e21424.
Crossref
Jia-Rong Fan, Ting-Hsiang Huang, Chen-Yu Wen, Tang-Long Shen & Tsai-Kun Li. (2010) Sodium salicylate acts through direct inhibition of phosphoinositide 3-kinase-like kinases to modulate topoisomerase-mediated DNA damage responses. European Journal of Pharmacology 638:1-3, pages 13-20.
Crossref
Wei Shi, Zhihui Feng, Jiuqin Zhang, Ignacio Gonzalez-Suarez, Robert P. Vanderwaal, Xiaohua Wu, Simon N. Powell, Joseph L. Roti Roti, Susana Gonzalo & Junran Zhang. (2010) The role of RPA2 phosphorylation in homologous recombination in response to replication arrest. Carcinogenesis 31:6, pages 994-1002.
Crossref
Alexander E. Ochem, Hocine Rechreche, Doris Skopac & Arturo Falaschi. (2008) Stimulation of the DNA unwinding activity of human DNA helicase II/Ku by phosphorylation. Archives of Biochemistry and Biophysics 470:1, pages 1-7.
Crossref
Y. Koyanagi, Y. Tanaka, M. Ito & N. Yamamoto. 2008. Humanized Mice. Humanized Mice 133 148 .
Frederick W. Alt. (2007) From gene amplification to V(D)J recombination and back: A personal account of my early years in B cell biology. European Journal of Immunology 37:S1, pages S138-S147.
Crossref
Erin Olson, Christian J. Nievera, Vitaly Klimovich, Ellen Fanning & Xiaohua Wu. (2006) RPA2 Is a Direct Downstream Target for ATR to Regulate the S-phase Checkpoint. Journal of Biological Chemistry 281:51, pages 39517-39533.
Crossref
Eric Hendrickson, Joy Huffman & John Tainer. 2005. DNA Damage Recognition. DNA Damage Recognition.
Yiyong Liu, Mamuka Kvaratskhelia, Sonja Hess, Youxing Qu & Yue Zou. (2005) Modulation of Replication Protein A Function by Its Hyperphosphorylation-induced Conformational Change Involving DNA Binding Domain B. Journal of Biological Chemistry 280:38, pages 32775-32783.
Crossref
Spencer J Collis, Theodore L DeWeese, Penelope A Jeggo & Antony R Parker. (2004) The life and death of DNA-PK. Oncogene 24:6, pages 949-961.
Crossref
Patrick Revy, Dietke Buck, Françoise le Deist & Jean‐Pierre de Villartay. 2005. 237 295 .
Francoise Le Deist, Catherine Poinsignon, Despina Moshous, Alain Fischer & Jean-Pierre de Villartay. (2004) Artemis sheds new light on V(D)J recombination. Immunological Reviews 200:1, pages 142-155.
Crossref
Sara K. Binz, Anne M. Sheehan & Marc S. Wold. (2004) Replication Protein A phosphorylation and the cellular response to DNA damage. DNA Repair 3:8-9, pages 1015-1024.
Crossref
Jason J. Millership, Xiaomin Cai & Guan Zhu. (2004) Functional characterization of replication protein A2 (RPA2) from Cryptosporidium parvum. Microbiology 150:5, pages 1197-1205.
Crossref
Despina Moshous & Jean-Pierre de Villartay. 2004. DNA Repair in Cancer Therapy. DNA Repair in Cancer Therapy 273 293 .
Olivier Guipaud, Ludovic Deriano, Hélène Salin, Laurent Vallat, Laure Sabatier, Hélène Merle-Béral & Jozo Delic. (2003) B-cell chronic lymphocytic leukaemia: a polymorphic family unified by genomic features. The Lancet Oncology 4:8, pages 506-514.
Crossref
Lan Ko & William W. Chin. (2003) Nuclear Receptor Coactivator Thyroid Hormone Receptor-binding Protein (TRBP) Interacts with and Stimulates Its Associated DNA-dependent Protein Kinase. Journal of Biological Chemistry 278:13, pages 11471-11479.
Crossref
Nam Hoon Cho, Carlos Cordon-Cardo, G. C. Li & See Hyun Kim. (2002) Allotype imbalance or microsatellite mutation in low-grade soft tissue sarcomas of the extremities in adults. The Journal of Pathology 198:1, pages 21-29.
Crossref
Takayuki Kobayashi, Shusuke Tada, Takashi Tsuyama, Hiromu Murofushi, Masayuki Seki & Takemi Enomoto. (2002) Focus-formation of replication protein A, activation of checkpoint system and DNA repair synthesis induced by DNA double-strand breaks in Xenopus egg extract . Journal of Cell Science 115:15, pages 3159-3169.
Crossref
Chris Allen, Akihiro Kurimasa, Mark A. Brenneman, David J. Chen & Jac A. Nickoloff. (2002) DNA-dependent protein kinase suppresses double-strand break-induced and spontaneous homologous recombination. Proceedings of the National Academy of Sciences 99:6, pages 3758-3763.
Crossref
T. M. Michaelidis & I. Grummt. (2002) Mechanism of Inhibition of RNA Polymerase I Transcription by DNA-dependent Protein Kinase. Biological Chemistry 383:11.
Crossref
Daniela Frasca, Luisa Guidi & Gino Doria. 2002. Basic Biology and Clinical Impact of Immunosenescence. Basic Biology and Clinical Impact of Immunosenescence 217 230 .
Paola Grandi, Michail Eltsov, Inga Nielsen & Ivan Raška. (2001) DNA double-strand breaks induce formation of RP-A/Ku foci on in vitro reconstituted Xenopus sperm nuclei . Journal of Cell Science 114:18, pages 3345-3357.
Crossref
Daniela Frasca, Paola Barattini, Grazia Tocchi, Luisa Guidi, Luca Pierelli & Gino Doria. (2001) Role of DNA-dependent protein kinase in recognition of radiation-induced DNA damage in human peripheral blood mononuclear cells. International Immunology 13:6, pages 791-797.
Crossref
Pauline Douglas, Greg B.G. Moorhead, Ruiqiong Ye & Susan P. Lees-Miller. (2001) Protein Phosphatases Regulate DNA-dependent Protein Kinase Activity. Journal of Biological Chemistry 276:22, pages 18992-18998.
Crossref
Eileen M. Lynch, Robert B. Moreland, Irene Ginis, Susan P. Perrine & Douglas V. Faller. (2001) Hypoxia-activated ligand HAL-1/13 is lupus autoantigen Ku80 and mediates lymphoid cell adhesion in vitro. American Journal of Physiology-Cell Physiology 280:4, pages C897-C911.
Crossref
Daniela Frasca, Paola Barattini, Grazia Tocchi, Francesco Guidi, Salvatore Scarpaci, Luisa Guidi, Carlo Bartoloni, Andrea Errani, Mario Costanzo & Gino Doria. (2001) Modulation of X-ray-induced damage recognition and repair in ageing human peripheral blood mononuclear cells by an interleukin-6-type cytokine. Mechanisms of Ageing and Development 121:1-3, pages 5-19.
Crossref
Manabu Koike, Tadahiro Shiomi & Aki Koike. (2000) Ku70 Can Translocate to the Nucleus Independent of Ku80 Translocation and DNA-PK Autophosphorylation. Biochemical and Biophysical Research Communications 276:3, pages 1105-1111.
Crossref
Jang-Su Park, Su-Jung Park, Xiaodong Peng, Mu Wang, Myeong-Ae Yu & Suk-Hee Lee. (1999) Involvement of DNA-dependent Protein Kinase in UV-induced Replication Arrest. Journal of Biological Chemistry 274:45, pages 32520-32527.
Crossref
Galit Rotman & Yosef Shiloh. (1999) ATM: A mediator of multiple responses to genotoxic stress. Oncogene 18:45, pages 6135-6144.
Crossref
Toyo Okui, Daiji Endoh & Masanobu Hayashi. (1999) Deficiency in fast repair process of potentially lethal damage induced by X-irradiation in fibroblasts derived from LEC strain rats. Mutation Research/DNA Repair 435:1, pages 81-88.
Crossref
A. Nilsson, F. Sirzén, R. Lewensohn, N. Wang & S. Skog. (1999) Cell cycle-dependent regulation of the DNA-dependent protein kinase. Cell Proliferation 32:4, pages 239-248.
Crossref
O. Osipovich, R.J. Duhe, P. Hasty, S.K. Durum & K. Muegge. (1999) Defining Functional Domains of Ku80: DNA End Binding and Survival after Radiation. Biochemical and Biophysical Research Communications 261:3, pages 802-807.
Crossref
C. Hennequin, N. Giocanti, D. Averbeck & V. Favaudon. (1999) La protéine kinase dépendante de l'ADN (DNA-PK), une enzyme clé de la religation des cassures double-brin de l'ADN. Cancer/Radiothérapie 3:4, pages 289-295.
Crossref
Kai Treuner, Marco Findeisen, Ulrich Strausfeld & Rolf Knippers. (1999) Phosphorylation of Replication Protein A Middle Subunit (RPA32) Leads to a Disassembly of the RPA Heterotrimer. Journal of Biological Chemistry 274:22, pages 15556-15561.
Crossref
Patrick Calsou, Philippe Frit, Odile Humbert, Catherine Muller, David J. Chen & Bernard Salles. (1999) The DNA-dependent Protein Kinase Catalytic Activity Regulates DNA End Processing by Means of Ku Entry into DNA. Journal of Biological Chemistry 274:12, pages 7848-7856.
Crossref
Balu R Chakravarthy, Teena Walker, Ingrid Rasquinha, Irene E Hill & John P MacManus. (2008) Activation of DNA-Dependent Protein Kinase May Play a Role in Apoptosis of Human Neuroblastoma Cells. Journal of Neurochemistry 72:3, pages 933-942.
Crossref
Isabelle Salles-Passador, Arun Fotedar & Rati Fotedara. (1999) Cellular response to DNA damage. Link between p53 and DNA-PK. Comptes Rendus de l'Académie des Sciences - Series III - Sciences de la Vie 322:2-3, pages 113-120.
Crossref
Molly A. Bogue, Chamelli Jhappan & David B. Roth. (1998) Analysis of variable (diversity) joining recombination in DNAdependent protein kinase (DNA-PK)-deficient mice reveals DNA-PK-independent pathways for both signal and coding joint formation. Proceedings of the National Academy of Sciences 95:26, pages 15559-15564.
Crossref
Shailendra Kumar, Pramod Pandey, Ajit Bharti, Shengfang Jin, Ralph Weichselbaum, David Weaver, Donald Kufe & Surender Kharbanda. (1998) Regulation of DNA-dependent Protein Kinase by the Lyn Tyrosine Kinase. Journal of Biological Chemistry 273:40, pages 25654-25658.
Crossref
Y. Yamaguchi, T. Wada, F. Suzuki, T. Takagi, J. Hasegawa & H. Handa. (1998) Casein kinase II interacts with the bZIP domains of several transcription factors. Nucleic Acids Research 26:16, pages 3854-3861.
Crossref
Sang Eun Lee, J.Kent Moore, Allyson Holmes, Keiko Umezu, Richard D Kolodner & James E Haber. (1998) Saccharomyces Ku70, Mre11/Rad50, and RPA Proteins Regulate Adaptation to G2/M Arrest after DNA Damage. Cell 94:3, pages 399-409.
Crossref
Shou Waga & Bruce Stillman. (1998) THE DNA REPLICATION FORK IN EUKARYOTIC CELLS. Annual Review of Biochemistry 67:1, pages 721-751.
Crossref
Nicholas S.Y. Ting, Peter N. Kao, Doug W. Chan, Lauri G. Lintott & Susan P. Lees-Miller. (1998) DNA-dependent Protein Kinase Interacts with Antigen Receptor Response Element Binding Proteins NF90 and NF45. Journal of Biological Chemistry 273:4, pages 2136-2145.
Crossref
Martin F. Lavin. 1998. Apoptosis: Mechanisms and Role in Disease. Apoptosis: Mechanisms and Role in Disease 213 232 .
P. A. Jeggo. 1998. DNA Damage and Repair. DNA Damage and Repair 317 334 .
Tak W. Mak & John J. L. Simard. 1998. Handbook of Imune Response Genes. Handbook of Imune Response Genes 109 170 .
Tak W. Mak & John J. L. Simard. 1998. Handbook of Imune Response Genes. Handbook of Imune Response Genes 67 107 .
Shengfang Jin, Surender Kharbanda, Bruce Mayer, Donald Kufe & David T. Weaver. (1997) Binding of Ku and c-Abl at the Kinase Homology Region of DNA-dependent Protein Kinase Catalytic Subunit. Journal of Biological Chemistry 272:40, pages 24763-24766.
Crossref
Maria Zernik-Kobak, Kersi Vasunia, Margery Connelly, Carl W. Anderson & Kathleen Dixon. (1997) Sites of UV-induced Phosphorylation of the p34 Subunit of Replication Protein A from HeLa Cells. Journal of Biological Chemistry 272:38, pages 23896-23904.
Crossref
Eileen L. Beall & Donald C. Rio. (1997) Drosophila P-element transposase is a novel site-specific endonuclease . Genes & Development 11:16, pages 2137-2151.
Crossref
Marc S. Wold. (1997) REPLICATION PROTEIN A: A Heterotrimeric, Single-Stranded DNA-Binding Protein Required for Eukaryotic DNA Metabolism. Annual Review of Biochemistry 66:1, pages 61-92.
Crossref
P.A Jeggo. (1997) DNA-PK: at the cross-roads of biochemistry and genetics. Mutation Research/DNA Repair 384:1, pages 1-14.
Crossref
Scott R. Peterson, Murray Stackhouse, Mary Jo Waltman, Fanqing Chen, Koki Sato & David J. Chen. (1997) Characterization of Two DNA Double-stranded Break Repair-deficient Cell Lines That Express Inactive DNA-dependent Protein Kinase Catalytic Subunits. Journal of Biological Chemistry 272:15, pages 10227-10231.
Crossref
Elizabeth T. Snow. (1997) The role of DNA repair in development. Reproductive Toxicology 11:2-3, pages 353-365.
Crossref
Michael R Lieber, Ulf Grawunder, Xiantuo Wu & Mariana Yaneva. (1997) Tying loose ends: roles of Ku and DNA-dependent protein kinase in the repair of double-strand breaks. Current Opinion in Genetics & Development 7:1, pages 99-104.
Crossref
Martin Gellert. 1997. Advances in Immunology Volume 64. Advances in Immunology Volume 64 39 64 .
Laura M. Fried, Constantinos Koumenis, Scott R. Peterson, Susannah L. Green, Pierre van Zijl, Joan Allalunis-Turner, David J. Chen, Richard Fishel, Amato J. Giaccia, J. Martin Brown & Cordula U. Kirchgessner. (1996) The DNA damage response in DNA-dependent protein kinase-deficient SCID mouse cells: Replication protein A hyperphosphorylation and p53 induction. Proceedings of the National Academy of Sciences 93:24, pages 13825-13830.
Crossref
Patrick Calsou, Philippe Frit & Bernard Salles. (1996) Double Strand Breaks in DNA Inhibit Nucleotide Excision Repair in Vitro. Journal of Biological Chemistry 271:44, pages 27601-27607.
Crossref
Luigi D Notarangelo. (1996) Immunodeficiencies caused by genetic defects in protein kinases. Current Opinion in Immunology 8:4, pages 448-453.
Crossref
S. P. Lees-Miller. (1996) The DNA-dependent protein kinase, DNA-PK: 10 years and no ends in sight. Biochemistry and Cell Biology 74:4, pages 503-512.
Crossref
Kevin McConnell & William S Dynan. (1996) The DNA-dependent protein kinase: a matter of life and (cell) death. Current Opinion in Cell Biology 8:3, pages 325-330.
Crossref
Molly Bogue & David B Roth. (1996) Mechanism of V(D)J recombination. Current Opinion in Immunology 8:2, pages 175-180.
Crossref
M A Bogue, C Zhu, E Aguilar-Cordova, L A Donehower & D B Roth. (1996) p53 is required for both radiation-induced differentiation and rescue of V(D)J rearrangement in scid mouse thymocytes.. Genes & Development 10:5, pages 553-565.
Crossref
Stephen P. Jackson. (1996) The recognition of DNA damage. Current Opinion in Genetics & Development 6:1, pages 19-25.
Crossref
Carl W. Anderson & Timothy H. Carter. 1996. Molecular Analysis of DNA Rearrangements in the Immune System. Molecular Analysis of DNA Rearrangements in the Immune System 91 111 .
Stephen Desiderio, Weei-Chin Lin & Zhong Li. 1996. Molecular Analysis of DNA Rearrangements in the Immune System. Molecular Analysis of DNA Rearrangements in the Immune System 45 59 .

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.