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Review

Bacterial ghosts as carrier and targeting systems for mucosal antigen delivery

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Pages 45-51 | Published online: 09 Jan 2014

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (9)

Siamak Alizadeh, Abolghasem Esmaeili, Abolfazl Barzegari, Mohammad A. Rafi & Yadollah Omidi. (2020) Bioengineered smart bacterial carriers for combinational targeted therapy of solid tumours. Journal of Drug Targeting 28:7-8, pages 700-713.
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Ali M. Batah & Tarek A. Ahmad. (2020) The development of ghost vaccines trials. Expert Review of Vaccines 19:6, pages 549-562.
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Jacqueline Montanaro, Aleksandra Inic-Kanada, Angela Ladurner, Elisabeth Stein, Sandra Belij, Nora Bintner, Simone Schlacher, Nadine Schuerer, Ulrike Beate Mayr, Werner Lubitz, Nikolaus Leisch & Talin Barisani-Asenbauer. (2015) Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface. Drug Design, Development and Therapy 9, pages 3741-3754.
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Abbas Muhammad, Jonathan Champeimont, Ulrike Beate Mayr, Werner Lubitz & Pavol Kudela. (2012) Bacterial ghosts as carriers of protein subunit and DNA-encoded antigens for vaccine applications. Expert Review of Vaccines 11:1, pages 97-116.
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Anne L Bishop & Andrew Camilli. (2011) Vibrio cholerae: lessons for mucosal vaccine design. Expert Review of Vaccines 10:1, pages 79-94.
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Timo Langemann, Verena Juliana Koller, Abbas Muhammad, Pavol Kudela, Ulrike Beate Mayr & Werner Lubitz. (2010) The bacterial ghost platform system. Bioengineered Bugs 1:5, pages 326-336.
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Eva M Riedmann, Jennelle M Kyd, Allan W Cripps & Werner Lubitz. (2007) Bacterial ghosts as adjuvant particles. Expert Review of Vaccines 6:2, pages 241-253.
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Susanne Paukner, Thomas Stiedl, Pavol Kudela, Jozef Bizik, Firas Al Laham & Werner Lubitz. (2006) Bacterial ghosts as a novel advanced targeting system for drug and DNA delivery. Expert Opinion on Drug Delivery 3:1, pages 11-22.
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Alain R Dumont, Lina H Kalfayan & Rafick-P Sékaly. (2004) Modulation of immune responses – strategies for optimising vaccines. Expert Opinion on Biological Therapy 4:5, pages 627-630.
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Articles from other publishers (52)

Harresh Adikesavalu, Thangapalam Jawahar Abraham & Siddhartha Narayan Joardar. (2022) Immune responses and protective immunity in Pangasius pangasius (Hamilton, 1822) as induced by outer membrane proteins of Edwardsiella tarda and aluminium hydroxide adjuvant complex. Veterinary Research Communications 46:4, pages 1097-1109.
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Sae-Ryung Kang, Dinh-Huy Nguyen, Su Woong Yoo & Jung-Joon Min. (2022) Bacteria and bacterial derivatives as delivery carriers for immunotherapy. Advanced Drug Delivery Reviews 181, pages 114085.
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Mounir M. Salem-Bekhit, Abdullah M. E. Youssof, Fars K. Alanazi, Fadilah Sfouq Aleanizy, Alsuwyeh Abdulaziz, Ehab I. Taha & Amro Abd Al Fattah Amara. (2021) Bacteria from Infectious Particles to Cell Based Anticancer Targeted Drug Delivery Systems. Pharmaceutics 13:12, pages 1984.
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Mohammad Nazmul Hasan, S.M. Shatil Shahriar, Jagannath Mondal, Md Nurunnabi & Yong-kyu Lee. 2021. Bioinspired and Biomimetic Materials for Drug Delivery. Bioinspired and Biomimetic Materials for Drug Delivery 89 104 .
Sukanta Kumar Nayak, Jyoti Prakash Dash & Pranabkanti Dutta. 2021. Biotechnological Advances in Aquaculture Health Management. Biotechnological Advances in Aquaculture Health Management 79 100 .
Safoura Soleymani, Amin Tavassoli, Gholamreza Hashemi Tabar, Gholam Ali Kalidari & Hesam Dehghani. (2020) Design, development, and evaluation of the efficacy of a nucleic acid-free version of a bacterial ghost candidate vaccine against avian pathogenic E. coli (APEC) O78:K80 serotype. Veterinary Research 51:1.
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Shengle Ji, Qiyang Gong, Wenwen Zhang, Jun Zheng, Bo Peng & Menghua Yang. (2020) Recombinant Vibrio parahaemolyticus ghosts protect zebrafish against infection by Vibrio species. Fish & Shellfish Immunology 107, pages 64-72.
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J. Daniel Griffin, Jimmy Y. Song, Joshua O. Sestak, Brandon J. DeKosky & Cory J. Berkland. (2020) Linking autoantigen properties to mechanisms of immunity. Advanced Drug Delivery Reviews 165-166, pages 105-116.
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Na Jiang, Lin Luo, Wei Xing, Tieliang Li, Ding Yuan, Guanling Xu, Wentong Li, Zhihong Ma, Liangyun Jin & Man Ji. (2019) Generation and immunity effect evaluation of biotechnology-derived Aeromonas veronii ghost by PhiX174 gene E-mediated inactivation in koi (Cyprinus carprio koi). Fish & Shellfish Immunology 86, pages 327-334.
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Rui Hou, Muzi Li, Tingting Tang, Ruichong Wang, Yijing Li, Yigang Xu, Lijie Tang, Li Wang, Min Liu, Yanping Jiang, Wen Cui & Xinyuan Qiao. (2018) Construction of Lactobacillus casei ghosts by Holin-mediated inactivation and the potential as a safe and effective vehicle for the delivery of DNA vaccines. BMC Microbiology 18:1.
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Fatemeh Farjadian, Mohsen Moghoofei, Soroush Mirkiani, Amir Ghasemi, Navid Rabiee, Shima Hadifar, Ali Beyzavi, Mahdi Karimi & Michael R. Hamblin. (2018) Bacterial components as naturally inspired nano-carriers for drug/gene delivery and immunization: Set the bugs to work?. Biotechnology Advances 36:4, pages 968-985.
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Abdulaziz Abdullah Alsuwyeh, Fars Alanazi, Faiyaz Shakeel, Mounir M. Salem-Bekhit & Nazrul Haq. (2017) Estimation of Anti-neoplastic Drug Doxorubicin in Bacterial Ghost Matrix by New “Environmentally Benign” RP-HPLC Method: A Step Towards Sustainable Development of Pharmaceutical Industry. Arabian Journal for Science and Engineering 43:1, pages 181-190.
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Irshad A. Hajam, Pervaiz A. Dar, Gayeon Won & John Hwa Lee. (2017) Bacterial ghosts as adjuvants: mechanisms and potential. Veterinary Research 48:1.
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Gayeon Won, Tae Hoon Kim & John Hwa Lee. (2017) A novel Salmonella strain inactivated by a regulated autolysis system and expressing the B subunit of Shiga toxin 2e efficiently elicits immune responses and confers protection against virulent Stx2e-producing Escherichia coli. BMC Veterinary Research 13:1.
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B. Pomeroy, A. Gurjar, A. Sipka, S. Klaessig, S. Salmon, R. Quesnell & Y.H. Schukken. (2016) Intramammary immunization with ultraviolet-killed Escherichia coli shows partial protection against late gestation intramammary challenge with a homologous strain. Journal of Dairy Science 99:11, pages 9014-9026.
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Abdullah M.E. Youssof, Mounir M. Salem-Bekhit, Faiyaz Shakeel, Fars K. Alanazi & Nazrul Haq. (2016) Analysis of anti-neoplastic drug in bacterial ghost matrix, w/o/w double nanoemulsion and w/o nanoemulsion by a validated ‘green’ liquid chromatographic method. Talanta 154, pages 292-298.
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Jianhua Li, Kun Xiong, Lingyun Zou, Zhijin Chen, Yiran Wang, Xiaomei Hu, Xiancai Rao & Yanguang Cong. (2015) Lytic Action of the Truncated yncE Gene in Escherichia coli. Current Microbiology 72:4, pages 390-396.
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I HUSSAIN, S RAJKHOWA, D HASIN & S IQBAL. (2015) Bacterial ghosts and their potential future applications-A mini review. The Indian Journal of Animal Sciences 85:12.
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M.R. Miri, A. Behzad-Behbahani, M. Fardaei, A. Farhadi, Y. Talebkhan, M. Mohammadi, M. Tayebinia, F. Farokhinejad, P. Alavi, M. Fanian, F. Zare, J. Saberzade, N. Nikouyan, M.A. Okhovat, R. Ranjbaran, G. Rafiei Dehbidi & S. Naderi. (2015) Construction of bacterial ghosts for transfer and expression of a chimeric hepatitis C virus gene in macrophages. Journal of Microbiological Methods 119, pages 228-232.
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Jun Liu, Yi Li, Yang Sun, Xue Ji, Lingwei Zhu, Xuejun Guo, Wei Zhou, Bo Zhou, Shuang Liu, Ruian Zhang & Shuzhang Feng. (2015) Immune responses and protection induced by Brucella suis S2 bacterial ghosts in mice. Veterinary Immunology and Immunopathology 166:3-4, pages 138-144.
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Wenxing Zhu, Yuyu Zhang & Xinli Liu. (2015) Efficient production of safety-enhanced Escherichia coli ghosts by tandem expression of PhiX 174 mutant gene E and staphylococcal nuclease A gene. Microbiological Research 176, pages 7-13.
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Jihua Guo & Rong Jia. (2013) A novel inducible expression system for the functional study of toxic gene in bacteria. World Journal of Microbiology and Biotechnology 30:5, pages 1527-1531.
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Amro A. Amara, Mounir M. Salem-Bekh & Fars K. Alanazi. (2013) Preparation of Bacterial Ghosts for E. coli JM109 Using “Sponge-like Reduced Protocol”. Asian Journal of Biological Sciences 6:8, pages 363-369.
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Mingming Hu, Yanhe Zhang, Fang Xie, Gang Li, Jianjun Li, Wei Si, Siguo Liu, Shouping Hu, Zhuo Zhang, Nan Shen & Chunlai Wang. (2013) Protection of Piglets by a Haemophilus parasuis Ghost Vaccine against Homologous Challenge. Clinical and Vaccine Immunology 20:6, pages 795-802.
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Kris Vande Walle, Daisy Vanrompay & Eric Cox. (2013) Bovine innate and adaptive immune responses against Escherichia coli O157:H7 and vaccination strategies to reduce faecal shedding in ruminants. Veterinary Immunology and Immunopathology 152:1-2, pages 109-120.
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Atul A Chaudhari, Chetan V Jawale, Sam Woong Kim & John Hwa Lee. (2012) Construction of a Salmonella Gallinarum ghost as a novel inactivated vaccine candidate and its protective efficacy against fowl typhoid in chickens. Veterinary Research 43:1.
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D.A. Vilte, M. Larzábal, U.B. Mayr, S. Garbaccio, M. Gammella, B.C. Rabinovitz, F. Delgado, V. Meikle, R.J.C. Cantet, P. Lubitz, W. Lubitz, A. Cataldi & E.C. Mercado. (2012) A systemic vaccine based on Escherichia coli O157:H7 bacterial ghosts (BGs) reduces the excretion of E. coli O157:H7 in calves. Veterinary Immunology and Immunopathology 146:2, pages 169-176.
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Ulrike Beate Mayr, Pavol Kudela, Alena Atrasheuskaya, Eugenij Bukin, Georgy Ignatyev & Werner Lubitz. (2011) Rectal single dose immunization of mice with Escherichia coli O157:H7 bacterial ghosts induces efficient humoral and cellular immune responses and protects against the lethal heterologous challenge . Microbial Biotechnology 5:2, pages 283-294.
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Anika C. Jahns & Bernd H. A. Rehm. (2011) Relevant uses of surface proteins – display on self‐organized biological structures. Microbial Biotechnology 5:2, pages 188-202.
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J. Liu, W.D. Wang, Y.J. Liu, S. Liu, B. Zhou, L.W. Zhu, X. Ji, Y. Sun & S.Z. Feng. (2012) Mice vaccinated with enteropathogenic Escherichia coli ghosts show significant protection against lethal challenges. Letters in Applied Microbiology 54:3, pages 255-262.
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Carmen Gutiérrez Millán, Clara Isabel Colino Gandarillas, María Luisa Sayalero Marinero & José M Lanao. (2012) Cell-based drug-delivery platforms. Therapeutic Delivery 3:1, pages 25-41.
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Wenxing Zhu, Guiwen Yang, Yuyu Zhang, Jinduo Yuan & Liguo An. (2012) Generation of Biotechnology-Derived Flavobacterium columnare Ghosts by PhiX174 Gene E -Mediated Inactivation and the Potential as Vaccine Candidates against Infection in Grass Carp . Journal of Biomedicine and Biotechnology 2012, pages 1-8.
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Holger Loessner, Astrid Schwantes, Matthias Hamdorf, Uliana Komor, Sara Leschner & Siegfried Weiss. 2012. Development of Novel Vaccines. Development of Novel Vaccines 87 124 .
Seung Hyuk Choi, Yoon Kwon Nam & Ki Hong Kim. (2010) Novel Expression System for Combined Vaccine Production in Edwardsiella tarda Ghost and Cadaver Cells. Molecular Biotechnology 46:2, pages 127-133.
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Alexander Sulakvelidze & Gary R. Pasternack. 2010. Bacteriophages in the Control of Food- and Waterborne Pathogens. Bacteriophages in the Control of Food- and Waterborne Pathogens 297 326 .
J. Solano-Parada, G. Gonzalez-Gonzalez, L.M. de Pablos Torró, M.F. Brazil dos Santos, A.M. Espino, M. Burgos & A. Osuna. (2010) Effectiveness of intranasal vaccination against Angiostrongylus costaricensis using a serine/threonine phosphatase 2 A synthetic peptide and recombinant antigens. Vaccine 28:32, pages 5185-5196.
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F.P. Tu, W.H. Chu, X.Y. Zhuang & C.P. Lu. (2010) Effect of oral immunization with Aeromonas hydrophila ghosts on protection against experimental fish infection. Letters in Applied Microbiology 50:1, pages 13-17.
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Xuepeng Wang & Chengping Lu. (2009) Mice orally vaccinated with Edwardsiella tarda ghosts are significantly protected against infection. Vaccine 27:10, pages 1571-1578.
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Petra Walcher, Xianlan Cui, Jane A. Arrow, Susie Scobie, Frank C. Molinia, Phil E. Cowan, Werner Lubitz & Janine A. Duckworth. (2008) Bacterial ghosts as a delivery system for zona pellucida-2 fertility control vaccines for brushtail possums (Trichosurus vulpecula). Vaccine 26:52, pages 6832-6838.
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Mahendrasingh Ramjeet, Vincent Deslandes, Julien Gouré & Mario Jacques. (2008) Actinobacillus pleuropneumoniae vaccines: from bacterins to new insights into vaccination strategies . Animal Health Research Reviews 9:1, pages 25-45.
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Herbert Hoffelner, Gabriele Rieder & Rainer Haas. (2008) Helicobacter pylori vaccine development: Optimisation of strategies and importance of challenging strain and animal model. International Journal of Medical Microbiology 298:1-2, pages 151-159.
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Se Ryun Kwon, Eun Hye Lee, Yoon Kwon Nam, Sung Koo Kim & Ki Hong Kim. (2007) Efficacy of oral immunization with Edwardsiella tarda ghosts against edwardsiellosis in olive flounder (Paralichthys olivaceus). Aquaculture 269:1-4, pages 84-88.
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Richard I. Walker, Duncan Steele & Teresa Aguado. (2007) Analysis of strategies to successfully vaccinate infants in developing countries against enterotoxigenic E. coli (ETEC) disease. Vaccine 25:14, pages 2545-2566.
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Ulrike Beate Mayr, Christoph Haller, Wolfgang Haidinger, Alena Atrasheuskaya, Eugenij Bukin, Werner Lubitz & Georgy Ignatyev. (2005) Bacterial Ghosts as an Oral Vaccine: a Single Dose of Escherichia coli O157:H7 Bacterial Ghosts Protects Mice against Lethal Challenge . Infection and Immunity 73:8, pages 4810-4817.
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Ulrike Beate Mayr, Petra Walcher, Chakameh Azimpour, Eva Riedmann, Christoph Haller & Werner Lubitz. (2005) Bacterial ghosts as antigen delivery vehicles. Advanced Drug Delivery Reviews 57:9, pages 1381-1391.
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Richard I. Walker. (2005) Considerations for development of whole cell bacterial vaccines to prevent diarrheal diseases in children in developing countries. Vaccine 23:26, pages 3369-3385.
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Chakameh Azimpour Tabrizi, Petra Walcher, Ulrike Beate Mayr, Thomas Stiedl, Matthias Binder, John McGrath & Werner Lubitz. (2004) Bacterial ghosts – biological particles as delivery systems for antigens, nucleic acids and drugs. Current Opinion in Biotechnology 15:6, pages 530-537.
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