Advanced search
Publication Cover
Stain Technology Volume 42, 1967 - Issue 5
Journal homepage
16
Views
98
CrossRef citations to date
0
Altmetric
Original Article

Embedding Media for 1–2 Micron Sectioning. 2. Hydroxyethyl Methacrylate Combined with 2-Butoxyethanol

Craig L. Ruddell College of Fisheries, University of Washington, Seattle, Washington, 98105
Pages 253-255 | Published online: 12 Jul 2009

Citations (98)

Keep up to date with the latest research on this topic with citation updates for this article.

Subscribe to citation updates

Read on this site (20)

Neil M. Hand & David Blythe. (2016) Plastic embedding of bone marrow trephine biopsies for routine immunohistochemistry and diagnosis: our developments, updates and experiences over 20 years. Journal of Histotechnology 39:4, pages 135-146.
Read now
Heba Elazab Mohamed Elazab. (2016) Pollen dimorphism in Acacia asak (Forssk.) Willd. (Fabaceae: Mimosoideae). Palynology 40:2, pages 193-201.
Read now
Peter O. Gerrits & Richard W. Horobin. (1996) Glycol Methacrylate Embedding for Light Microscopy: Basic Principles and Trouble-Shooting. Journal of Histotechnology 19:4, pages 297-311.
Read now
Peter O. Gerrits & Bernhard Eppinger. (1995) Effects of Storing Initiated 2-Hydroxyethyl Methacrylate Solutions for Embedding Tissues for Light Microscopy: Some Practical Implications. Biotechnic & Histochemistry 70:3, pages 155-163.
Read now
Jean-Pierre Montheard, Michel Chatzopoulos & Daniel Chappard. (1992) 2-Hydroxyethyl Methacrylate (HEMA): Chemical Properties and Applications in Biomedical Fields. Journal of Macromolecular Science, Part C 32:1, pages 1-34.
Read now
Roger A. Brumback, Daniel L. Feeback, Richard W. Leech, Judy L. Ketring & Julie J. Davis. (1989) Modification of Brain Processing Techniques: Tissue Embedding in Glycol Methacrylate and Stain Modifications. Journal of Histotechnology 12:3, pages 201-207.
Read now
Nithyananda A. Nathan & A. van Deth. (1985) Comparative Evaluation of Diagnostic Cytological Features of Malignancy in GMA Sections, Paraffin Sections and Smears. Pathology 17:3, pages 467-473.
Read now
Daniel G. Gomez, Joseph D. Fenstermacher, Rene P. Manzo, Dennis Johnson & D. Gordon Pcyits. (1985) Cerebrospinal Fluid Absorption in the Rabbit: Olfactory Pathways. Acta Oto-Laryngologica 100:5-6, pages 429-436.
Read now
P. O. Gerrits. (1983) A Simple Method to Handle Tacky Glycol Methacrylate Blocks. Stain Technology 58:2, pages 120-120.
Read now
Elizabeth M. McDowell, John W. Combs & Carnell Newkirk. (1983) A Quantitative Light and Electron Microscopic Study of Hamster Tracheal Epithelium with Special Attention to So-Called Intermediate Cells. Experimental Lung Research 4:3, pages 205-226.
Read now
Danny J. Smith. (1981) Improved Masson Trichrome Stain on Plastic-Embedded Tissue. Journal of Histotechnology 4:3, pages 132-133.
Read now
H. P. Godfrey, J. Pastore & P. W. Askenase. (1981) A Modified Giemsa Stain for Demonstrating Basophils in Glycol Methacrylate-Embedded Tissue Sections. Journal of Histotechnology 4:2, pages 80-82.
Read now
Henry Troyer & Elaine Babich. (1981) A Hematoxylin and Eosin-Like Stain for Glycol Methacrylate Embedded Tissue Sections. Stain Technology 56:1, pages 39-43.
Read now
Richard M. Franklin & Maria-Teresa Martin. (1980) Staining and Histochemistry of Undecalcified Bone Embedded in a Water-Miscible Plastic. Stain Technology 55:5, pages 313-321.
Read now
Nathan T. Brinn & John Phillip Pickett. (1979) Glycol Methacrylate for Routine, Special Stains, Histochemistry, Enzyme Histochemistry and Immunohistochemistry: A Simplified Cold Method for Surgical Biopsy Tissue. Journal of Histotechnology 2:3, pages 125-130.
Read now
Clyde N. Lulham. (1979) Glycol Methacrylate Embedding or Light Microscopy. Journal of Histotechnology 2:2, pages 68-71.
Read now
Robin Frater. (1979) Rapid Removal of Acid from Glycol Methacrylate for Improved Histological Embedding. Stain Technology 54:5, pages 241-243.
Read now
Akira Senoo. (1978) A New Preparation Method For Several Histopathological Examinations On A Single Block. Stain Technology 53:3, pages 123-129.
Read now
Madison B. Cole & Stephen M. Sykes. (1974) Glycol Methacrylate in Light Microscopy a Routine Method for Embedding and Sectioning Animal Tissues. Stain Technology 49:6, pages 387-400.
Read now
Harold C. Grice & Robert G. Carlson. (1972) The Changing Role Of Pathology In Modern Safety Evaluation. CRC Critical Reviews in Toxicology 1:2, pages 119-152.
Read now

Articles from other publishers (78)

John W. Stirling & Anthony E. Woods. 2019. Bancroft's Theory and Practice of Histological Techniques. Bancroft's Theory and Practice of Histological Techniques 96 113 .
Neil M. Hand. 2013. Bancroft's Theory and Practice of Histological Techniques. Bancroft's Theory and Practice of Histological Techniques 139 155 .
M. Neumüller & W. Hartmann. (2008) The phenotypically quantitative nature of hypersensitivity of European plum (Prunus domestica L.) against the Plum pox virus and its description using the hypersensitivity index. Horticultural Science 35:2, pages 50-64.
Crossref
Neil M. Hand. 2008. Theory and Practice of Histological Techniques. Theory and Practice of Histological Techniques 585 599 .
Yashuharu Noishiki, Xiao Hua Ma, Yoshihisa Yamane, Shinichi Satoh, Takafumi Okoshi, Kazuhiro Takahashi, Yoshihiro Iwai, Takayuki Kosuge, Yukio Ichikawa, Ichiya Yamazaki & Makoto Mo. (2002) Succinylated Collagen Crosslinked by Thermal Treatment for Coating Vascular Prostheses. Artificial Organs 22:8, pages 672-680.
Crossref
Yasuharu Noishiki, Yasuko Tomizawa, Yoshihisa Yamane, Takafumi Okoshi, Shinichi Satoh, Akihiko Matsumoto & D. Craig Miller. (1993) Acceleration of neointima formation in vascular prostheses by transplantation of autologous venous tissue fragments. The Journal of Thoracic and Cardiovascular Surgery 105:5, pages 796-804.
Crossref
Peter O. Gerrits, Richard W. Horobin & David J. Wright. (2011) Staining sections of water‐miscible resins. Journal of Microscopy 160:3, pages 279-290.
Crossref
Marie F. Rouleau, Jane Mitchell & David Goltzman. (2009) Characterization of the major parathyroid hormone target cell in the endosteal metaphysis of rat long bones. Journal of Bone and Mineral Research 5:10, pages 1043-1053.
Crossref
P. O. Gerrits, R. W. Horobin & M. J. Hardonk. (1990) A numerical procedure for choosing effective, low toxicity plasticizers for glycol methacrylate embedding. The Histochemical Journal 22:8, pages 439-451.
Crossref
Elizabeth M. McDowell, William P. Coleman, Andrea M. De Santi, Carnell Newkirk & Judy M. Strum. (2005) Development of the conducting airway epithelium in fetal Syrian golden hamsters during normal and diabetic pregancies. The Anatomical Record 227:1, pages 111-123.
Crossref
Robert E. Sigler, Carnell Newkirk & Elizabeth M. McDowell. (1988) Histogenesis and morphogenesis of epidermoid metaplasia in hamster tracheal organ explant culture. Virchows Archiv B Cell Pathology 55:1, pages 47-55.
Crossref
P. Bianco, P. Ballanti & E. Bonucci. (1988) Tartrate-resistant acid phosphatase activity in rat osteoblasts and osteocytes. Calcified Tissue International 43:3, pages 167-171.
Crossref
P. Bianco, M. Costantini, L. C. Dearden & E. Bonucci. (2008) Alkaline phosphatase positive precursors of adipocytes in the human bone marrow. British Journal of Haematology 68:4, pages 401-403.
Crossref
R. I. S. Agbede & D. H. Kemp. (1987) Boophilus microplus (ixodid tick): Fine structure of the gut basophilic cell in relation to water and ion transport. Experimental & Applied Acarology 3:3, pages 233-242.
Crossref
Robert E. Sigler, Raymond T. Jones, John R. Hebel & Elizabeth M. McDowell. (1987) Hamster tracheal organ culture in serum-free media: A quantitative comparison of in vitro epithelial morphology with that of in vivo controls. In Vitro Cellular & Developmental Biology 23:2, pages 100-110.
Crossref
Judy M. Strum, Patricia S. Latham, Martin L. Schmidt & Elizabeth M. McDowell. (1986) Vitamin A deprivation in hamsters. Virchows Archiv B Cell Pathology Including Molecular Pathology 50:1, pages 43-57.
Crossref
James H. Resau, Kosaku Sakamoto, John R. Cottrell & Carnell Newkirk. (1986) Explant organ culture of hamster alimentary tract epithelium. Cell Biology and Toxicology 2:3, pages 401-415.
Crossref
R.I.S. Agbede & D.H. Kemp. (1986) Immunization of cattle against Boophilus microplus using extracts derived from adult female ticks: Histopathology of ticks feeding on vaccinated cattle. International Journal for Parasitology 16:1, pages 35-41.
Crossref
Elaine M. Otani, Carnell Newkirk & Elizabeth M. McDowell. (2005) Development of hamster tracheal epithelium: IV. Cell proliferation and cytodifferentiation in the neonate. The Anatomical Record 214:2, pages 183-192.
Crossref
S. Fink. (1986) A new integrated concept for the improved preparation of sections of fresh or frozen tissue for light microscope histochemistry. Histochemistry 86:1, pages 43-52.
Crossref
Elizabeth M. McDowell & Theodore F. BealsElizabeth M. Mcdowell & Theodore F. Beals. 1986. Biopsy Pathology of the Bronchi. Biopsy Pathology of the Bronchi 23 48 .
Elizabeth M. McDowell, Carnell Newkirk & Bill Coleman. (2005) Development of hamster tracheal epithelium: I. A quantitative morphologic study in the fetus. The Anatomical Record 213:3, pages 429-447.
Crossref
P. O. Gerrits & M. B. M. van Leeuwen. (2011) Glycol methacrylate embedding in histotechnology: factors which influence the evolution of heat during polymerization at room temperature. Journal of Microscopy 139:3, pages 303-311.
Crossref
Victor Hugo Carabba, Sergei Pitirimovitch Sorokin & Richard Francis HoytJr.Jr.. (2005) Development of neuroepithelial bodies in intact and cultured lungs of fetal rats. American Journal of Anatomy 173:1, pages 1-27.
Crossref
R.I.S. Agbede & D.H. Kemp. (1985) Digestion in the cattle-tick Boophilus microplus: Light microscope study of the gut cells in nymphs and females. International Journal for Parasitology 15:2, pages 147-157.
Crossref
G. H. J. Lewis & I. D. Bowen. (1985) A methacrylate embedding technique for combined autoradiography and acid phosphatase histochemistry. The Histochemical Journal 17:4, pages 467-475.
Crossref
R. S. Poethig & I. M. Sussex. (1985) The developmental morphology and growth dynamics of the tobacco leaf. Planta 165:2, pages 158-169.
Crossref
CURTIS CHUBB & CATHERINE NOLAN. (1984) Genetic Control of Steroidogenesis and Spermatogenesis in Inbred Mice. Annals of the New York Academy of Sciences 438:1 Hormonal Cont, pages 519-522.
Crossref
Michael BromleyDavid E. Woolley. (2005) Histopathology of the rheumatoid lesion. Arthritis & Rheumatism 27:8, pages 857-863.
Crossref
J. E. Den Hollander & D. A. Erasmus. (2009) Schistosoma mansoni : DNA synthesis in males and females from mixed and single-sex infections . Parasitology 88:3, pages 463-476.
Crossref
Allan D. Pearsall, Robert Echt, Lawrence M. Ross, Robert A. RothJr.Jr. & Robert J. Dinerstein. (2005) Morphologic and cytochemical characteristics of amine‐containing globule leukocytes in rat tracheal epithelium. American Journal of Anatomy 170:1, pages 83-99.
Crossref
K. Von Loeffler & I. Bidier. (2007) Zur Frage der Umdifferenzierung der Knorpelzellen der Gelenkoberfläche am Beispiel der Synovialgrubenbildung*. Anatomia, Histologia, Embryologia 13:1, pages 68-85.
Crossref
Elizabeth M. McDowell, Kevin P. Keenan & Mark Huang. (1984) Effects of vitamin A-deprivation on hamster tracheal epithelium. Virchows Archiv B Cell Pathology Including Molecular Pathology 45:1, pages 197-219.
Crossref
Sergio Villaschi & Luigi G. Spagnoli. (1983) Autoradiographic and ultrastructural studies on the human fibro-atheromatous plaque. Atherosclerosis 48:1, pages 95-100.
Crossref
Kevin P. Keenan, John W. Combs & Elizabeth M. McDowell. (1983) Regeneration of hamster tracheal epithelium after mechanical injury. Virchows Archiv B Cell Pathology Including Molecular Pathology 41:1, pages 193-214.
Crossref
Johan G. Hanstede & Peter O. Gerrits. (2005) A new plastic for morphometric investigation of blood vessels, especially in large organs such as the human liver. The Anatomical Record 203:2, pages 307-315.
Crossref
P. L. MINGAZZINI, F. MALCHIODI ALBEDI & V. BLANDAMURA. (2007) Villous adenoma of the duodenum: cellular composition and histochemical findings. Histopathology 6:2, pages 235-244.
Crossref
Emiko OGUCHI & Koji SAKAMOTO. (1982) EFFECT OF A SINGLE, LARGE DOSE INJECTION OF PYRITHIAMIN ON THE THIAMIN DEFICIENCY OF THE MOUSE. Japanese Journal of Pharmacology 32:5, pages 775-783.
Crossref
I.J. Alexander & W.L. Bigg. (1981) Light microscopy of ectomycorrhizas using glycol methacrylate. Transactions of the British Mycological Society 77:2, pages 425-429.
Crossref
Madison B. ColeJrJr & Joan Ellinger. (2011) Glycol methacrylate in light microscopy: nucleic acid cytochemistry. Journal of Microscopy 123:1, pages 75-88.
Crossref
Carlo D. Baroni, Liborio Manente, Massimo Occhionero, Antonella Marzullo, Franco Mandelli & Carissimo Biagini. (2018) Involvement of the Bone Marrow by Non-Hodgkin's Lymphomas: Incidence, Histology and Pathologic Correlations. Tumori Journal 67:3, pages 191-196.
Crossref
W. Hermanns, K. Liebig & L. -C. Schulz. (1981) Postembedding immunohistochemical demonstration of antigen in experimental polyarthritis using plastic embedded whole joints. Histochemistry 73:3, pages 439-446.
Crossref
H. Westen, K. -F. M�ck & L. Post. (1981) Enzyme histochemistry on bone marrow sections after embedding in methacrylate at low temperature. Histochemistry 70:2, pages 95-105.
Crossref
W. Hermanns & L. C. Schulz. 1981. Arthritis. Arthritis 137 143 .
Richard M. Franklin & Maria-Teresa Martin. (1980) In ovo tumorigenesis induced by avian osteopetrosis virus. Virology 105:1, pages 245-249.
Crossref
H.H. Sedlacek, Rosemarie Wanger & H.-J. Bengelsdorff. (1980) Influence of Various Immunomodulators on the Induction of Experimental Autoimmune Encephalomyelitis in Lewis Rats. Immunobiology 157:2, pages 99-108.
Crossref
Carlo D. Baroni & Fiorella Malchiodi. (1980) Histology, age and sex distribution, and pathologic correlations of Hodgkin's disease. A study of 184 cases observed in rome, italy. Cancer 45:7, pages 1549-1555.
Crossref
F. R. Seiler & H.-H. Sedlacek. 1980. Cancer Chemo- and Immunopharmacology. Cancer Chemo- and Immunopharmacology 53 60 .
Lawrence W. Kaler & William B. Neaves. (2005) Attrition of the human leydig cell population with advancing age. The Anatomical Record 192:4, pages 513-518.
Crossref
Sergei P. Sorokin & Richard F. HoytJr.Jr.. (2005) PAS‐lead hematoxylin as a stain for small‐granule endocrine cell populations in the lungs, other pharyngeal derivatives and the gut. The Anatomical Record 192:2, pages 245-259.
Crossref
J W Menzie, L H Hoffman & A M Michelakis. (1978) Immunofluorescent localization of renin in mouse submaxillary gland and kidney.. American Journal of Physiology-Endocrinology and Metabolism 234:5, pages E480.
Crossref
E. Jaros & W.G. Bradley. (1978) Development of the amyelinated lesion in the ventral root of the dystrophic mouse. Journal of the Neurological Sciences 36:3, pages 317-339.
Crossref
Yutaka Takasaki. (1978) Studies on brain lesion by administration of monosodium L-glutamate to mice. I. Brain lesions in infant mice caused by administration of monosodium L-glutamae. Toxicology 9:4, pages 293-305.
Crossref
K.C. Binnington. (1978) Sequential changes in salivary gland structure during attachment and feeding of the cattle tick, Boophilus microplus. International Journal for Parasitology 8:2, pages 97-115.
Crossref
D. K. Kido, D. G. Gomez, Ch. A. Santos-Buch, T. V. Caston & D. G. Potts. (1978) Microradiographic study of cerebral and ocular aneurysms in hypertensive rabbits. Neuroradiology 15:1, pages 21-26.
Crossref
G. H. Green. (1977) 2-Hydroxyethyl methacrylate for sectioning bone marrow. Journal of Clinical Pathology 30:1, pages 90-91.
Crossref
Li‐Tsun Chen & Evelyn E. Handler. (2005) An electron microscopic study of the spleen of the rat in an acute myelogenous leukemia. The Anatomical Record 187:1, pages 29-45.
Crossref
Fred R. Nusbickel & Henry Troyer. (2005) Histochemical investigation of adjuvant‐induced arthritis. Arthritis & Rheumatism 19:6, pages 1339-1346.
Crossref
D. K. Kido, D. G. Gomez, A. M. PaveseJr.Jr. & D. G. Potts. (1976) Human spinal arachnoid villi and granulations. Neuroradiology 11:5, pages 221-228.
Crossref
J T Dancey, K A Deubelbeiss, L A Harker & J Greenaway. (1976) Section preparation of human marrow for light microscopy.. Journal of Clinical Pathology 29:8, pages 704-710.
Crossref
Barbara F. Matlaga. (2016) Glycol Methacrylate: Embedding, Sectioning, and Staining for Light Microscopy. The Bulletin of the Society of Pharmacological and Environmental Pathologists 4:2, pages 9-11.
Crossref
. (2016) News From the Society. The Bulletin of the Society of Pharmacological and Environmental Pathologists 4:2, pages 1-1.
Crossref
Anton G. Endress & William W. Thomson. (1976) Ultrastructural and cytochemical studies on the developing adhesive disc of Boston Ivy tendrils. Protoplasma 88:2-4, pages 315-331.
Crossref
M. B. ColeJrJr. (1976) Holding plastic-embedded specimens for sectioning in a rotary microtome. Experientia 32:5, pages 674-675.
Crossref
Peter M. Andrews. (1975) Scanning electron microscopy of the nephrotic kidney. Virchows Archiv B Cell Pathology 17:1, pages 195-211.
Crossref
Sergei P. Sorokin & Joseph D. Brain. (2005) Pathways of clearance in mouse lungs exposed to iron oxide aerosols. The Anatomical Record 181:3, pages 581-625.
Crossref
D. G. Gomez, A. A. Chambers, A. T. Di Benedetto & D. G. Potts. (1974) The spinal cerebrospinal fluid absorptive pathwaysLes voies d'absorption du liquide cephalo-rachidien au niveau du rachisWege der spinalen, Liquor-Absorption. Neuroradiology 8:2, pages 61-66.
Crossref
B. Sims. (2011) A simple method of preparing 1–2 μm sections of large tissue blocks using glycol methacrylate. Journal of Microscopy 101:2, pages 223-227.
Crossref
Balder P. Gloor. (1973) Zur Entwicklung des Glask�rpers und der ZonulaDevelopment of vitreous and zonule. Albrecht von Graefes Archiv f�r Klinische und Experimentelle Ophthalmologie 189:2, pages 105-124.
Crossref
Hedi Nemetschek-Gansler, G. Berg & O. Schiller. (1972) Erfahrungen mit einer neuen Fixierungs- und Einbettungsmethode für die Routine-Histologie. Beiträge zur Pathologie 147:2, pages 201-206.
Crossref
James A. Hightower & Ronald L. St. Pierre. (2005) Skin allografts in the newt, Notopthalmus viridescens: Cessation of blood flow as an indicator of rejection, . Journal of Experimental Zoology 181:3, pages 341-351.
Crossref
G�nter Weber. (1972) Glykolmethacrylat-Einbettung und 1?2 ?m-Schnitt-Technik f�r Zeckengewebe und ganze ZeckenGlycol methacrylate embedding and 1?2 ?m sectioning of tick tissues and whole ticks. Zeitschrift f�r Parasitenkunde 40:4, pages 295-306.
Crossref
James A. Hightower & Ronald L. St. Pierre. (2005) Hemopoietic tissue in the adult newt, Notopthalmus viridescens . Journal of Morphology 135:3, pages 299-307.
Crossref
Edward D. Blackburn, John E. King, William G. Pace & Neil R. Thomford. (1971) Morphologic changes in liver and kidney after in vivo freezing with cryoprobe. Cryobiology 8:4, pages 333-338.
Crossref
Charles F. Simpson. (2016) Fate of Erythrocytes Containing Heinz Bodies in the Spleen and Liver of Dogs and Turkeys. Veterinary Pathology 8:2, pages 118-129.
Crossref
Craig L. Ruddell. (1971) Elucidation of the nature and function of the granular oyster amebocytes through histochemical studies of normal and traumatized oyster tissues. Histochemie 26:2, pages 98-112.
Crossref
Neil R. Thomford, Wayne H. Wilson, Edward D. Blackburn & William G. Pace. (1970) Morphological changes in canine trachea after freezing. Cryobiology 7:1, pages 19-26.
Crossref
Craig L. Ruddell. (1969) The demonstration of anions generated by the action of sodium hypochlorite (NaOCl) on tissue sections, including observations on the unmasking of carbonyl groups. Histochemie 19:4, pages 319-339.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.