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Artificial Cells, Blood Substitutes, and Biotechnology Volume 40, 2012 - Issue 6
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Research Article

Covalent immobilization of trypsin on glutaraldehyde-activated silica for protein fragmentation

Cenk DagliogluEge University, Faculty of Science, Biochemistry Department, Bornova-Izmir, Turkey
&
Figen ZihniogluEge University, Faculty of Science, Biochemistry Department, Bornova-Izmir, TurkeyCorrespondence[email protected]
Pages 378-384 | Received 23 Mar 2012, Accepted 27 Mar 2012, Published online: 06 Jun 2012

Citations (15)

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Thu Bao Le, Chong Soo Han, Kyoungwon Cho & Oksoo Han. (2018) Covalent immobilization of oxylipin biosynthetic enzymes on nanoporous rice husk silica for production of cis(+)-12-oxophytodienoic acid. Artificial Cells, Nanomedicine, and Biotechnology 46:8, pages 1523-1529.
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Esra Menfaatli & Figen Zihnioglu. (2015) Carrier free immobilization and characterization of trypsin. Artificial Cells, Nanomedicine, and Biotechnology 43:2, pages 140-144.
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Articles from other publishers (13)

Elena N. Esimbekova, Irina G. Torgashina, Elena V. Nemtseva, Anna A. Antashkevich, Polina Yu. Sasova & Valentina A. Kratasyuk. (2023) Trypsin-Based Chemoenzymatic Assay for Detection of Pollutants and Safety Assessment of Food Additives. Chemosensors 11:4, pages 237.
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Shalu Aggarwal & Saiqa Ikram. (2022) Zinc oxide nanoparticles-impregnated chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies. International Journal of Biological Macromolecules 207, pages 205-221.
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Selmihan ŞAHİN & İsmail ÖZMEN. (2021) Immobilization and Characterization of Trypsin on TiO2 Nanoparticles Activated with Crosslinkers. Academic Platform Journal of Engineering and Science 9:2, pages 274-283.
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Antje Kumpf, Daria Kowalczykiewicz, Katarzyna Szymańska, Maria Mehnert, Isabel Bento, Aleksandra Łochowicz, André Pollender, Andrzej Jarzȩbski & Dirk Tischler. (2020) Immobilization of the Highly Active UDP-Glucose Pyrophosphorylase From Thermocrispum agreste Provides a Highly Efficient Biocatalyst for the Production of UDP-Glucose. Frontiers in Bioengineering and Biotechnology 8.
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Selmihan Sahin & Ismail Ozmen. (2020) Covalent immobilization of trypsin on polyvinyl alcohol-coated magnetic nanoparticles activated with glutaraldehyde. Journal of Pharmaceutical and Biomedical Analysis 184, pages 113195.
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Tanchanok Poonsin, Benjamin K. Simpson, Wonnop Visessanguan, Asami Yoshida & Sappasith Klomklao. (2020) Optimal immobilization of trypsin from the spleen of albacore tuna (Thunnus alalunga) and its characterization. International Journal of Biological Macromolecules 143, pages 462-471.
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Davide Piccinino, Eliana Capecchi, Lorenzo Botta, Paolo Bollella, Riccarda Antiochia, Marcello Crucianelli & Raffaele Saladino. (2019) Layer by layer supported laccase on lignin nanoparticles catalyzes the selective oxidation of alcohols to aldehydes. Catalysis Science & Technology 9:15, pages 4125-4134.
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Keziban Atacan, Ahmed Nuri Kursunlu & Mustafa Ozmen. (2019) Preparation of pillar[5]arene immobilized trypsin and its application in microwave-assisted digestion of Cytochrome c. Materials Science and Engineering: C 94, pages 886-893.
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Mariana Paola Cabrera, Taciano França da Fonseca, Raquel Varela Barreto de Souza, Caio Rodrigo Dias de Assis, Justiniano Quispe Marcatoma, Jackeline da Costa Maciel, David Fernando Morais Neri, Fernando Soria & Luiz Bezerra de CarvalhoJr.Jr.. (2018) Polyaniline-coated magnetic diatomite nanoparticles as a matrix for immobilizing enzymes. Applied Surface Science 457, pages 21-29.
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Keziban Atacan, Bekir Çakıroğlu & Mahmut Özacar. (2017) Covalent immobilization of trypsin onto modified magnetite nanoparticles and its application for casein digestion. International Journal of Biological Macromolecules 97, pages 148-155.
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Cenk Daglioglu & Burcu Okutucu. (2016) Synthesis and Characterization of AICAR and DOX Conjugated Multifunctional Nanoparticles as a Platform for Synergistic Inhibition of Cancer Cell Growth. Bioconjugate Chemistry 27:4, pages 1098-1111.
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Keziban Atacan & Mahmut Özacar. (2015) Characterization and immobilization of trypsin on tannic acid modified Fe3O4 nanoparticles. Colloids and Surfaces B: Biointerfaces 128, pages 227-236.
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Prashanth Ramachandran, Guru Krupa Narayanan, Sakthivel Gandhi, Swaminathan Sethuraman & Uma Maheswari Krishnan. (2014) Rhizopus oryzae Lipase Immobilized on Hierarchical Mesoporous Silica Supports for Transesterification of Rice Bran Oil. Applied Biochemistry and Biotechnology 175:5, pages 2332-2346.
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