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mAbs Volume 4, 2012 - Issue 6
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Characterization of antibody variants during process development: The tale of incomplete processing of N-terminal secretion peptide

alexandre Ambrogelly BioProcess Development; Merck Research Laboratories; Union, NJ USACorrespondence[email protected]
,
Yan-Hui Liu BioProcess Development; Merck Research Laboratories; Union, NJ USA; Molecular Biomarkers; Merck Research Laboratories; Kenilworth, NJ USA
,
Hong Li BioProcess Development; Merck Research Laboratories; Union, NJ USA
,
selina Mengisen BioProcess Development; Merck Research Laboratories; Union, NJ USA
,
bingyi Yao BioProcess Development; Merck Research Laboratories; Union, NJ USA
,
Wei Xu BioProcess Development; Merck Research Laboratories; Union, NJ USA
&
Susan Cannon-Carlson BioProcess Development; Merck Research Laboratories; Union, NJ USA
 show all
Pages 701-709 | Received 25 Apr 2012, Accepted 24 Jul 2012, Published online: 29 Aug 2012

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Douglas S. Rehder, Chris J. Wisniewski, Dengfeng Liu, Diya Ren, Dell Farnan & Matthew R. Schenauer. (2019) Expression vector-derived heterogeneity in a therapeutic IgG4 monoclonal antibody. mAbs 11:1, pages 145-152.
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Rabah Gahoual, Alicia Burr, Jean-Marc Busnel, Lauriane Kuhn, Phillipe Hammann, Alain Beck, Yannis-Nicolas François & Emmanuelle Leize-Wagner. (2013) Rapid and multi-level characterization of trastuzumab using sheathless capillary electrophoresis-tandem mass spectrometry. mAbs 5:3, pages 479-490.
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M. J. Traylor, A. V. Tchoudakova, A. M. Lundquist, J. E. Gill, F. L. Boldog & B. S. Tangarone. (2016) Comprehensive Discovery and Quantitation of Protein Heterogeneity via LC-MS/MS Peptide Mapping for Clone Selection of a Therapeutic Protein. Analytical Chemistry 88:18, pages 9309-9317.
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Hongcheng Liu, Christine Nowak, Mei Shao, Gomathinayagam Ponniah & Alyssa Neill. (2016) Impact of cell culture on recombinant monoclonal antibody product heterogeneity. Biotechnology Progress 32:5, pages 1103-1112.
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Hongbin Liu, Justin Jeong, Yung-Hsiang Kao & Yonghua Taylor Zhang. (2015) Characterization of free thiol variants of an IgG1 by reversed phase ultra high pressure liquid chromatography coupled with mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 109, pages 142-149.
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Daniel Ayoub, Diego Bertaccini, Hélène Diemer, Elsa Wagner-Rousset, Olivier Colas, Sarah Cianférani, Alain Van Dorsselaer, Alain Beck & Christine Schaeffer-Reiss. (2015) Characterization of the N-Terminal Heterogeneities of Monoclonal Antibodies Using In-Gel Charge Derivatization of α-Amines and LC-MS/MS. Analytical Chemistry 87:7, pages 3784-3790.
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David A. Michels, Anna Y. Ip, Thomas M. Dillon, Kurt Brorson, Scott Lute, Brittany Chavez, Ken M. Prentice, Lowell J. Brady & Karen J. Miller. 2015. State-of-the-Art and Emerging Technologies for Therapeutic Monoclonal Antibody Characterization Volume 2. Biopharmaceutical Characterization: The NISTmAb Case Study. State-of-the-Art and Emerging Technologies for Therapeutic Monoclonal Antibody Characterization Volume 2. Biopharmaceutical Characterization: The NISTmAb Case Study 237 284 .
Alain Beck, Elsa Wagner-Rousset, Daniel Ayoub, Alain Van Dorsselaer & Sarah Sanglier-Cianférani. (2012) Characterization of Therapeutic Antibodies and Related Products. Analytical Chemistry 85:2, pages 715-736.
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Michael J. Gramer. 2014. Mammalian Cell Cultures for Biologics Manufacturing. Mammalian Cell Cultures for Biologics Manufacturing 123 166 .

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