Duodenal mast cells and eosinophils in children with celiac disease: occurrence and distribution pattern

Abstract

Objective

The aim of this study was to characterize duodenal mast cell (MC) and eosinophil (EO) numbers, their distribution within the lamina propria and possible impact on disease severity of paediatric celiac patients compared to children without celiac disease (CD).

Methods

We analysed duodenal samples of 215 children (109 CD, 106 controls) who underwent esophagogastroduodenoscopy from 2010 to 2018. After immunohistochemical staining, average MC and EO counts were histologically examined in ten high-power-fields. Additionally, cell-distribution within the lamina propria was analysed. Possible influence of relevant clinical parameters was evaluated.

Statistics

Student’s-t-test, Mann–Whitney U-test, Chi-square-test, ANOVA, significance-level <.05. Trial registration-number: DRKS00024669.

Results

MC-density was higher in CD-patients compared to the control-group (23.7 (±12.1)/HPF versus 19.7 (±9.1)/HPF; p = .008), varying in number interindividually. Eosinophils were also increased in the duodenum of celiac patients (23.3 (±9.3)/HPF versus 12.2 (±6.3)/HPF; p= <.001). MCs were distributed more often homogenously in all parts of CD lamina propria (44 biopsies (40.4%), residing more distant from the intestinal lumen in controls (0 biopsies with homogenous distribution-pattern (0%); p= <.001). Regarding EOs no polarity was observable. Atopic diseases did not occur significantly more often in patients with elevated EO-counts.

Conclusion

MC- and EO-numbers were increased in the duodenum of CD-patients and MCs showed a different distribution-pattern in the lamina propria of celiac patients. These findings support the concept that both cell-types contribute to disease-pathogenesis. However, functional studies highlighting both cell-types’ and their mediators’ role regarding mucosal alterations during the course of the inflammatory process in celiac patients are needed.

Trial registration number and URL

DRKS00024669; https://www.drks.de/drks_web/

Keywords:

• Gluten-sensitive enteropathy
• Marsh classification
• inflammatory cells
• intestinal mucosa
• lamina propria
• celiac disease

Acknowledgements

The authors would like to acknowledge the technical assistants of the Institute of Pathology in Bochum.

Disclosure statement

No potential conflict of interest was reported by the author(s).