Insight into the fabrication, characterization, and in vitro cytotoxicity studies approaches of halloysite-based functional anhydride containing polymer nanocomposites

Abstract

This study aims to design, synthesize, and characterization of maleic anhydride-vinyl acetate and its halloysite nanotubes and to evaluate the cytotoxic effects of nanostructures on human gingival fibroblasts. Poly(maleic anhydride-alt-vinyl acetate) [Poly(MA-alt-VA)] was synthesized via charge transfer complex-radical polymerization as potential dental fillings, adhesives, or bone cement materials. ATR-FTIR, XPS, and XRD spectroscopic methods were used to enlighten the structure–property of nanomaterials. Determining surface area and pore volume BET was used. Morphological characterization was viewed by HRTEM. Cytotoxic effects of nanocomposites on cell viability were evaluated by MTT assay for different incubation periods and concentrations for human gingival fibroblasts.

Graphical Abstract

Keywords:

• Copolymer
• halloysite
• human gingival fibroblasts
• nanocomposite
• nanotube
• poly(MA-alt-VA)
• XPS

Disclosure statement

No potential conflict of interest was reported by the author(s).