Biotransformed Antioxidant isoflavone extracts present high-capacity to attenuate the in vitro formation of advanced glycation end products

ABSTRACT

Advanced glycation end products (AGEs) are toxic with a high capacity to cause damages to health, mainly associated with diabetes complications. In this study, soybean isoflavone extracts were subjected to different biotransformation processes: Lactobacilli fermentation, Enzymatic and Enzymatic followed by fermentation, in order to improve their antioxidant capacity and bioactive isoflavone content. The effects of the biotransformation processes on total phenolic content, antioxidant activities, and isoflavone profile were evaluated. Analyses of antiglycation activity of the isoflavones extracts were evaluated by glycation models in vitro. The results showed that all bioprocesses increased the aglycone content, as well as the antioxidant capacity by about 2.5 times using ORAC and 1.6 times using FRAP assays. The results were superior for enzymatic biotransformation followed by fermentation. All processes showed about 24,33% to 57,33% of anti-glycationt capacity, indicating that biotransformation improved the bioactivity of isoflavones extracts for glycation inhibitory activity, as well as the associated e antioxidant capacity.

KEYWORDS:

• Biotransformation
• antioxidant
• glycation products
• isoflavones
• bioactivity

Abbreviations

AGEs	=	Advanced glycation end products
ARG	=	Arginine
BSA	=	Bovine serum albumin
CFU	=	Colony formation unit
DPPH	=	2,2-diphenyl-1-picrylhydrazyl
FRAP	=	ferric reducing antioxidant power
FRU	=	Fructose
GAE	=	gallic acid equivalents
GO	=	Glyoxal
HPLC	=	high performance liquid chromatography
MGO	=	Methylglyoxal
ORAC	=	Oxygen radical absorbance capacity
TE	=	Trolox equivalents
Trolox	=	6-Hydroxy-2,5,7,8-tetramethylchromane-2- carboxylic acid
UV	=	Ultraviolet
v/v	=	volume/volume

Supplementary material

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Additional information

Funding

This work was supported by the São Paulo Research Foundation [FAPESP, finance code 2018/24868-3], the Brazilian National Council for Scientific and Technological Development [CNPq, finance code 140884/2016-5], and the Brazilian Federal Agency for Support and Evaluation of Graduate Education [CAPES] for the financial support.