White, brown, and bone marrow adipose tissue behavior in DHEA-induced PCOS mice

Abstract

Introduction: To investigate the behavior of white, brown, and bone marrow adipose tissue (MAT) and the insulin resistance in a PCOS mice model.

Methods

Thirty-one female C57BL/6J mice were divided into four groups: two were treated with subcutaneous dehydroepiandrosterone (DHEA) implants and divided into normal and hypercaloric diet (HFD). Two were control and divided into normal and HFD. Presence of insulin resistance, growth, and adipocyte markers expression of white and brown adipose tissues and growth and inflammatory cytokines expression of bone marrow adipose tissue were evaluated.

Results

Hypercaloric diet groups presented higher total weight gain and huge growth in all fat sites, except bone marrow. They also demonstrated greater expression of adipocyte markers in sites of white adipose tissue. DHEA + HFD group showed more insulin intolerance than all other groups. DHEA shows to abrogate AdipoQ expression in all fatty tissues.

Conclusions

DHEA alone does not influence adipose tissue growth, but contributes to increased insulin resistance and influences the expression of adipokines. Proximal MAT showed different behavior from the other fat depot.

摘要

前言：研究白色、棕色和骨髓脂肪组织（MAT）在多囊卵巢综合征（PCOS）小鼠模型中的行为和胰岛素抵抗。

方法：31只C57BL/6J雌性小鼠随机分为4组：2组皮下植入脱氢表雄酮（DHEA）, 分为正常饮食组和高热量饮食（HFD）组。另2例为对照组, 分为正常饮食组和HFD组。评估胰岛素抵抗、白色、棕色脂肪组织的生长和脂肪细胞标志物的表达, 以及骨髓脂肪组织的生长和炎性细胞因子的表达。

结果：高热量饮食组的总体重增加更高, 除骨髓脂肪组织外, 其它所有脂肪部位都有明显的增长。还显示白色脂肪组织部位脂肪细胞标记物的表达较高。DHEA+HFD组胰岛素抵抗程度明显高于其他各组。DHEA显示在所有脂肪组织中都能抑制AdipoQ的表达。

结论：DHEA本身并不影响脂肪组织的生长, 但会促进胰岛素抵抗增加, 并影响脂肪因子的表达。近端MAT表现行为不同于其他脂肪储备。

Keywords:

• Androgen
• adipose tissue
• insulin resistance
• adipokines

Acknowledgements

The authors thank Professor Laércio da Silva Paiva for his help with statistical analyses and interpretation of the data. The services of Tatiana Guida Ponce and Kayque Ferrari Fachi are appreciated.

Disclosure statement

The authors report no conflict of interest.

Additional information

Funding

This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (São Paulo Research Foundation – FAPESP) under Research Grant – Regular 2016/25244-8. L.D.S. received a grant for her Master’s degree funded by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brazil (CAPES) – Financing Code 001.