Bacterial-mediated biodegradation of pentachlorophenol via electron shuttling

ABSTRACT

Pentachlorophenol (PCP) degradation by soil indigenous bacteria represents a practical and cost-effective solution. In the present study, bacteria isolated from paddy soil was investigated and the role of electron shuttling (ES) in the PCP degradation process was assessed. Two strains demonstrated the highest PCP degradation of 93.5% and 94.88% in the presence of citrate and were identified using 16S rRNA phylogenetic analysis as Pseudomonas chengduensis and Pseudomonas plecoglossicida, respectively. Both strains showed higher PCP degradation in free form as opposed to a reduced activity in immobilized and respiratory impaired form. The addition of pyruvate resulted in about 80% PCP degradation in 5 days for P. chengduensis, on the other hand, P. plecoglossicida showed the same result under anaerobic conditions whether pyruvate was added or not. Phenazine and the outer membrane c-type cytochrome were reported only for P. chengduensis as opposed to P. plecoglossicida. The results indicate that despite following different approaches in PCP degradation, both strains are useful in PCP clean-up under aerobic and anaerobic conditions and in free direct contact. The degradation is enhanced via ES. This is considered both an effective and feasible technology for in situ clean-up of contaminated sites or on-site bioreactors.

KEYWORDS:

• Pseudomonas
• electron shuttling
• mediators
• pentachlorophenol
• biodegradation

Disclosure statement

No potential conflict of interest was reported by the authors.