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Article

Settled dust assessment in clinical environment: useful for the evaluation of a wider bioburden spectrum

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Pages 160-178 | Received 18 Mar 2019, Accepted 18 Jun 2019, Published online: 26 Jun 2019
 

ABSTRACT

The collection and analysis of settled dust samples from indoor environments has become one of several environmental sampling methods used to assess bioburden indoors. The aim of the study was to characterize the bioburden in vacuumed settled dust from 10 Primary Health Care Centers by culture based and molecular methods. Results for bacterial load ranged from 1 to 12 CFU.g−1 of dust and Gram-negative bacteria ranged between 1 to 344 CFU.g−1 of dust. Fungal load ranged from 0 CFU.g−1 of dust to uncountable. Aspergillus section Fumigati was detected in 4 sampling sites where culture base-methods could not identify this section. Mucorales (Rhizopus sp.) was observed on 1 mg/L voriconazole. Three out of 10 settled dust samples were contaminated by mycotoxins. Settled dust sampling coupled with air sampling in a routine way might provide useful information about bioburden exposure.

Acknowledgments

The authors are grateful to FCT – Fundação para Ciência e Tecnologia for funding the project EXPOsE – Establishing protocols to assess occupational exposure to microbiota in clinical settings (02/SAICT/2016 – Project nº 23222).

Conflict of Interest

None.

We have full control of all primary data and permission is given to the journal to review the data if requested.

Additional information

Funding

This work was supported by the Fundação para Ciência e Tecnologia [02/SAICT/2016 – Project nº 23222].

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