Settled dust assessment in clinical environment: useful for the evaluation of a wider bioburden spectrum

References

• Aleksic B, Draghi M, Ritoux S, Bailly S, Lacroix M, Oswald IP, Bailly J-D, Robinec E. 2017. Aerosolization of mycotoxins after growth of toxinogenic fungi on wallpaper. Appl Environ Microbiol. 83(16):1–12. doi:10.1128/AEM.00653-17
   Web of Science ®Google Scholar
• Arendrup MC, Rodriguez-Tudela JL, Lass-Flörl C, Cuenca-Estrella M, Donnelly JP, Hope W. 2013. EUCAST technical note on anidulafungin. Clin Microbiol Infect. 19(6):278–280. doi:10.1111/1469-0691.12148.
   Web of Science ®Google Scholar
• Assunção R, Vasco E, Nunes B, Loureiro S, Martins C, Alvito P. 2015. Single-compound and cumulative risk assessment of mycotoxins present in breakfast cereals consumed by children from Lisbon region, Portugal. Food Chem Toxicol. 86:274–281. https://linkinghub.elsevier.com/retrieve/pii/S0278691515300867.
   PubMed Web of Science ®Google Scholar
• Badyda A, Gayer A, Czechowski P, Majewski G, Dąbrowiecki P. 2016. Pulmonary function and incidence of selected respiratory diseases depending on the exposure to ambient PM10. Int J Mol Sci. 22 17(11): 1954. http://www.mdpi.com/1422-0067/17/11/1954.
   PubMed Web of Science ®Google Scholar
• Barberán A, Dunn RR, Reich BJ, Pacifici K, Laber EB, Menninger HL, Morton JM, Henley JB, Leff JW, Miller SL, et al. 2015. The ecology of microscopic life in household dust. Proc R Soc B Biol Sci. 7 282(1814):20151139. http://rspb.royalsocietypublishing.org/lookup/doi/10.1098/rspb.2015.1139.
   Web of Science ®Google Scholar
• Bergwall C, Stehn B. 2002. Comparison of selective mycological agar media for the isolation and enumeration of xerophilic moulds and osmotolerant yeasts in granulated white sugar. Zuckerindustrie. 127:259–264.
   Google Scholar
• Bouillard L, Michel O, Dramaix M, Devleeschouwer M. 2005. Bacterial contamination of indoor air, surfaces, and settled dust, and related dust endotoxin concentrations in healthy office buildings. Ann Agric Environ Med. 12(2):187–192. http://www.ncbi.nlm.nih.gov/pubmed/16457472.
   PubMed Web of Science ®Google Scholar
• Cabo Verde S, Almeida SM, Matos J, Guerreiro D, Meneses M, Faria T, Botelho D, Santos M, Viegas C. 2015. Microbiological assessment of indoor air quality at different hospital sites. Res Microbiol. doi:10.1016/j.resmic.2015.03.004
   PubMed Web of Science ®Google Scholar
• Chen Q, Hildemann LM. 2009. The effects of human activities on exposure to particulate matter and bioaerosols in residential homes. Environ Sci Technol. 43(13):4641–4646. doi:10.1021/es802296j.
   PubMed Web of Science ®Google Scholar
• Cox J, Indugula R, Vesper S, Zhu Z, Jandarov R, Reponen T. 2017. Comparison of indoor air sampling and dust collection methods for fungal exposure assessment using quantitative PCR. Environ Sci Process Impacts The Royal Soc Chem. 19(10):1312–1319. doi:10.1039/C7EM00257B.
   PubMed Web of Science ®Google Scholar
• Cruz-Perez P, Buttner MP, Stetzenbach LD. 2001. Detection and quantitation of Aspergillus fumigatus in pure culture using polymerase chain reaction. Mol Cell Probe. 15:81–88. doi:10.1006/mcpr.2000.0343
   PubMed Web of Science ®Google Scholar
• Dasgupta A. 2016. Personalized immunosuppression in transplantation role of biomarker monitoring and therapeutic drug monitoring. Pers Immunosuppression Transpl. (1st. Edition) 83–107.
   Google Scholar
• De Hoog GS, Guarro J, Gebé J, Figueras MJ. 2000. Atlas of Clinical Fungi. ISBN: 90-70351-43-9, 2nd ed. Utrecht (The Netherlands): Centraalbureau voor Schimmelcultures.
   Google Scholar
• Degois J, Clerc F, Simon X, Bontemps C, Leblond P, Duquenne P. 2017. First metagenomic survey of the microbial diversity in bioaerosols emitted in waste sorting plants. Ann Work Expo Heal. 61(9):1076–1086. doi:10.1093/annweh/wxx075.
   PubMed Web of Science ®Google Scholar
• Dunn RR, Fierer N, Henley JB, Leff JW, Menninger HL. 2013. Home life: factors structuring the bacterial diversity found within and between homes. Bertilsson S, editor. PLoS One. 22 8(5):e64133. doi:10.1371/journal.pone.0064133.
   PubMed Web of Science ®Google Scholar
• Eduard W, Halstensen AS. 2009. Quantitative exposure assessment of organic dust. Scand J Work Environ Heal Suppl. 7:30–35.
   Google Scholar
• Egbuta M, Mwanza M, Babalola O. 2017. Health Risks Associated with Exposure to Filamentous Fungi. Int J Environ Res Public Health. 4 14(7):719. http://www.mdpi.com/1660-4601/14/7/719.
   Web of Science ®Google Scholar
• Eliopoulos GM, Perea S, Patterson TF. 2002. Antifungal resistance in pathogenic fungi. Clin Infect Dis. 35(9):1073–1080. doi:10.1086/344058.
   PubMed Web of Science ®Google Scholar
• Emerson JB, Keady PB, Brewer TE, Clements N, Morgan EE, Awerbuch J, Miller SL, Fierer N. 2015. Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado front range flood. Environ Sci Technol. 3 49(5):2675–2684. doi:10.1021/es503845j.
   PubMed Web of Science ®Google Scholar
• EPA, United States Environmental Protection Agency 2017. About the national exposure research laboratory (NERL). [Accessed 2017 Jun 19]. http://www.epa.gov/nerlcwww/moldtech.htm〉.
   Google Scholar
• Flannigan B. 1997. Air sampling for fungi in indoor environments. J Aerosol Sci. 28(3):381–392. http://linkinghub.elsevier.com/retrieve/pii/S0021850296004417.
   Web of Science ®Google Scholar
• Frankel M, Timm M, Hansen EW, Madsen AM. 2012. Comparison of sampling methods for the assessment of indoor microbial exposure. Indoor Air. 22(5):405–414. doi:10.1111/ina.2012.22.issue-5.
   PubMed Web of Science ®Google Scholar
• Frisvad JC, Smedsgaard J, Larsen TO, Samson RA. 2004. Mycotoxins, drugs and other extrolites produced by species in Penicillium subgenus Penicillium. Stud Mycol. 49:201–241.
   Google Scholar
• Fröhlich-Nowoisky J, Pickersgill DA, Despres VR, Pöschl U. 2009. High diversity of fungi in air particulate matter. Proc Natl Acad Sci. 106:12814–12819. doi:10.1073/pnas.0811003106.
   PubMed Web of Science ®Google Scholar
• Gao W, Jiang L, Ge L, Chen M, Geng C, Yang G, Li Q, Ji F, Yan Q, Zou Y, et al. 2015. Sterigmatocysit-induced oxidative DNA damage in human liver-derived cell line through lysosomal damage. Toxicol in Vitro. 29:1–7. doi:10.1016/j.tiv.2014.08.007.
   PubMed Web of Science ®Google Scholar
• Gralton J, Tovey E, McLaws M-L, Rawlinson WD. 2011. The role of particle size in aerosolised pathogen transmission: A review. J Infect. 62(1):1–13. doi:10.1016/j.jinf.2010.11.010.
   PubMed Web of Science ®Google Scholar
• Halstensen AS, Nordby K-C, Eduard W, Klemsdal SS. 2006. Real-time PCR detection of toxigenic Fusarium in airborne and settled grain dust and associations with trichothecene mycotoxins. J Environ Monit. 8(12):1235. http://xlink.rsc.org/?DOI=b609840a.
   PubMedGoogle Scholar
• Haugland RA, Brinkman N, Vesper SJ. 2002. Evaluation of rapid DNA extraction methods for the quantitative detection of fungi using real-time PCR analysis. J Microbiol Methods. 50:319–323.
   PubMed Web of Science ®Google Scholar
• Haugland RA, Varma M, Wymer LJ, Vesper SJ. 2004. Quantitative PCR analysis of selected Aspergillus, Penicillium and Paecilomyces species. Syst Appl Microbiol. 27(2):198–210. doi:10.1078/072320204322881826.
   PubMed Web of Science ®Google Scholar
• Heikkila P, Salmi T, Kotimaa M. 1988. Identification and counting of fungal spores by scanning electron microscopy. Ann Occup Hyg. 32:241–248. doi:10.1093/annhyg/32.2.241.
   PubMedGoogle Scholar
• Heutte N, Andr� V, Dubos Arvis C, Bouchart V, Lemari� F, Legendre P, Votier E, Louis MY, Madelaine S2, Séguin V, et al. 2017. Assessment of multi-contaminant exposure in a cancer treatment center: a 2-year monitoring of molds, mycotoxins, endotoxins, and glucans in bioaerosols. Environ Monit Assess. 189(1). doi:10.1007/s10661-016-5751-z.
   PubMed Web of Science ®Google Scholar
• Hicks JB, Lu ET, De Guzman R, Weingart M. 2005. Fungal types and concentrations from settled dust in normal residences. J Occup Environ Hyg. 1 2(10):481–492. doi:10.1080/15459620500252860.
   PubMed Web of Science ®Google Scholar
• Hindson BJ, Ness KD, Masquelier DA, Belgrader P, Heredia NJ, Makarewicz AJ, Bright IJ, Lucero MY, Hiddessen AL, Legler TC. et al. 2011. High-throughput droplet digital PCR system for absolute quantitation of DNA Copy Number. Anal Chem. 15 83(22):8604–8610. Doi:10.1021/ac202028g
   PubMed Web of Science ®Google Scholar
• Hodgson M, Scott R. 1999. Prevalence of fungi in carpet dust samples. In: Johanning E, editor. Bioaerosols, fungi and mycotoxins: health effects, assessment, prevention and control. Albany (N.Y): Boyd Printing Company; p. 268–274.
   Google Scholar
• Horak B, Dutkiewicz J, Solarz K. 1996. Microflora and acarofauna of bed dust from homes in Upper Silesia, Poland. Ann Allergy. Asthma Immunol. 76(1):41–50. http://linkinghub.elsevier.com/retrieve/pii/S1081120610634059.
   PubMed Web of Science ®Google Scholar
• Kettleson EM, Adhikari A, Vesper S, Coombs K, Indugula R, Reponen T. 2015. Key determinants of the fungal and bacterial microbiomes in homes. Environ Res. 138:130–135. doi:10.1016/j.envres.2015.02.003
   PubMed Web of Science ®Google Scholar
• Klánová K, Hollerová J. 2003. Hospital indoor environment: screening for micro-organisms and particulate matter. Ndoor Built Environ. 12(1–2):61–67. doi:10.1177/1420326X03012001010.
   Web of Science ®Google Scholar
• Kleinkauf N, Verweij P, Arendrup M, Donnelly P, Cuenca-Estrella M, Fraaije B, Melchers W, Adriaenssens N, Kema G, Ullmann A, et al. 2013. Risk assessment on the impact of environmental usage of triazoles on the development and spread of resistance to medical triazoles in Aspergillus species. Stockholm. doi:10.2900/76274
   PubMedGoogle Scholar
• Lamoth F. 2016. Aspergillus fumigatus-related species in clinical practice. Front Microbiol. 7:683. doi:10.3389/fmicb.2016.00683.
   PubMed Web of Science ®Google Scholar
• Latge JP. 1999. Aspergillus fumigatus and aspergillosis. Clin Microbiol Rev. 12:310–350. doi:10.1128/CMR.12.2.310.
   PubMed Web of Science ®Google Scholar
• Lax S, Smith DP, Hampton-Marcell J, Owens SM, Handley KM, Scott NM, Gibbons SM, Larsen P, Shogan BD, Weiss S, Metcalf JL, Ursell LK, Vázquez-Baeza Y. et al. 2014. Longitudinal analysis of microbial interaction between humans and the indoor environment. Science. 29 345(6200):1048–1052. doi:10.1126/science.1254529
   PubMed Web of Science ®Google Scholar
• Leppänen HK, Nevalainen A, Vepsäläinen A, Roponen M, Täubel M, Laine O, Rantakokko P, von Mutius E, Pekkanen J, Hyvärinen A. 2014. Determinants, reproducibility, and seasonal variation of ergosterol levels in house dust. Indoor Air. 24(3):248–259. doi:10.1111/ina.12078.
   PubMed Web of Science ®Google Scholar
• Leppänen HK, Täubel M, Jayaprakash B, Vepsäläinen A, Pasanen P, Hyvärinen A. 2018. Quantitative assessment of microbes from samples of indoor air and dust. J Expo Sci Environ Epidemiol. 4 28(3):231–241. doi:10.1038/jes.2017.24
   PubMed Web of Science ®Google Scholar
• Leung M, Chan AHS. 2006. Control and management of hospital indoor air quality. Med Sci Monit. 12(3):SR17–SR23. http://www.ncbi.nlm.nih.gov/pubmed/16501436.
   PubMed Web of Science ®Google Scholar
• Liu Y, Du M, Zhang G. 2014. Proapoptotic activity of aflatoxin B 1 and sterigmatocystin in HepG2 cells. Toxicol Reports. 1:1076–1086. https://linkinghub.elsevier.com/retrieve/pii/S2214750014001139.
   PubMedGoogle Scholar
• Macedo D, Leonardelli F, Dudiuk C Theill L, Cabeza MS, Gamarra S, Garcia-Effron G.2018. Molecular Confirmation of the Linkage between the Rhizopus oryzae CYP51A gene coding region and its intrinsic voriconazole and fluconazole resistance. Antimicrob Agents Chemother. 27 62(8). doi:10.1128/AAC.00224-18.
   PubMed Web of Science ®Google Scholar
• Marchand G, Duchaine C, Lavoie J, Veillette M, Cloutier Y. 2016. Bacteria emitted in ambient air during bronchoscopy—a risk to health care workers? Am J Infect Control. 44(12):1634–1638. https://linkinghub.elsevier.com/retrieve/pii/S0196655316304989.
   PubMed Web of Science ®Google Scholar
• Marchand G, Lacombe N, Pépin C. 2017. Évaluation de la biomasse mycologique sur les surfaces des réseaux aérauliques des systèmes de ventilation. Quebec: Institut de recherche Robert-Sauvé en santé et en sécurité du travail. (Rapports Scientifiques: DesLibris). https://books.google.pt/books?id=4A_BswEACAAJ.
   Google Scholar
• Martins C, Assunção R, Cunha SC, Fernandes JO, Jager A, Petta T, Oliveira CA, Alvito P. 2018. Assessment of multiple mycotoxins in breakfast cereals available in the Portuguese market. Food Chem. 239:132–140. https://linkinghub.elsevier.com/retrieve/pii/S0308814617310701.
   PubMed Web of Science ®Google Scholar
• Mayer Z, Bagnara A, FaÅNrber P, Geisen R. 2003. Quantification of the copy number of nor-1, a gene of the aflatoxin biosynthetic pathway by real-time PCR, and its correlation to the cfu of Aspergillus flavus in foods. Int J Food Microbiol. 82:143–151. doi:10.1016/S0168-1605(02)00250-7
   PubMed Web of Science ®Google Scholar
• McConnell IR, Garner RC. 1994. DNA adducts of aflatoxins, sterigmatocystin and other mycotoxins. IARC Sci Publ. (125):49–55. http://www.ncbi.nlm.nih.gov/pubmed/7806340.
   PubMedGoogle Scholar
• Meadow JF, Altrichter AE, Kembel SW, Kline J, Mhuireach G, Moriyama M, Northcutt D, O'Connor TK, Womack AM, Brown GZ. et al. 2014. Indoor airborne bacterial communities are influenced by ventilation, occupancy, and outdoor air source. Indoor Air. 24(1):41–48. doi:10.1111/ina.12047.
   PubMed Web of Science ®Google Scholar
• Meyer HW, Würtz H, Suadicani P, Valbjørn O, Sigsgaard T, Gyntelberg F. 2004. Molds in floor dust and building-related symptoms in adolescent school children. Indoor Air. 14(1):65–72. doi:10.1046/j.1600-0668.2003.00213.x.
   PubMed Web of Science ®Google Scholar
• Perlin DS.2017. Stop neglecting fungi. Nat Microbiol. 25;2(8):17120. http://www.nature.com/articles/nmicrobiol2017120.
   Google Scholar
• Nevalainen A, Täubel M, Hyvärinen A. 2015. Indoor fungi: companions and contaminants. Indoor Air. 25(2):125–156. doi:10.1111/ina.12182.
   PubMed Web of Science ®Google Scholar
• Osman M, Ibrahim H, Yousef F, Elnasr AA, Saeed Y, Hameed AA. 2018. A study on microbiological contamination on air quality in hospitals in Egypt. Indoor Built Environ. 2 27(7):953–968. doi:10.1177/1420326X17698193.
   Web of Science ®Google Scholar
• Park DU, Yeom JK, Lee WJ, Lee KM. 2013. Assessment of the levels of airborne bacteria, gram-negative bacteria, and fungi in hospital lobbies. Int J Environ Res Public Health. 10(2):541–555. doi:10.3390/ijerph10020541.
   PubMed Web of Science ®Google Scholar
• Park J-H, Sulyok M, Lemons AR, Green BJ, Cox-Ganser JM. 2018. Characterization of fungi in office dust: comparing results of microbial secondary metabolites, fungal internal transcribed spacer region sequencing, viable culture and other microbial indices. Indoor Air. 28(5):708–720. doi:10.1111/ina.2018.28.issue-5.
   Web of Science ®Google Scholar
• Reponen T. 2017. Sampling for microbial determination. In: In VC, Viegas S, Quintal GA, Taubel M, Sabino R, editors. Exposure to microbiological agents in indoor and occupational environments. SpringerNature. 85–96. doi:10.1007/978-3-319-61688-9
   Google Scholar
• Ruiz-Camps I, Aguado JM, Almirante B, Bouza E, Ferrer-Barbera CF, Len O, Lopez-Cerero L, Rodríguez-Tudela JL, Ruiz M, Solé A. et al. 2011. Guidelines for the prevention of invasive mould diseases caused by filamentous fungi by the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC). Clin Microbiol Infect. 7:1–24. doi:10.1111/j.1469-0691.2011.03477.x
   Google Scholar
• Samson RA, Hoekstra ES, Frisvad JC 2000. Introduction to food- and airborne fungi 6th rev. e. Utrecht : Centraalbureau voor schimmelcultures. http://lib.ugent.be/catalog/rug01:000698898.
   Google Scholar
• Sandle T, Vijayakumar R, Saleh Al Aboody M, Saravanakumar S. 2014. vitro fungicidal activity of biocides against pharmaceutical environmental fungal isolates. J Appl Microbiol. 117:1267–1273. doi:10.1111/jam.12525.
   PubMed Web of Science ®Google Scholar
• Sarıca S, Asan A, Otkun MT, Ture M. 2002. Monitoring indoor airborne fungi and bacteria in the different areas of trakya university hospital, Edirne, Turkey. Indoor Built Environ. 11:285–292. doi:10.1159/000066523
   Web of Science ®Google Scholar
• Schrader C, Schielke A, Ellerbroek L, Johne R. 2012. PCR inhibitors - occurrence, properties and removal. J Appl Microbiol. 113(5):1014–1026. doi:10.1111/jam.2012.113.issue-5.
   PubMed Web of Science ®Google Scholar
• Shen YE, Sorenson WG, Lewis DM, Olenchock SA. 1990. Microbiological analyses and inflammatory effects of settled dusts from rice and hay. Biomed Environ Sci.3. 3:353–363. http://www.ncbi.nlm.nih.gov/pubmed/2252555.
   PubMedGoogle Scholar
• Srikanth P, Sudharsanam S, Steinberg R. 2008. Bio-aerosols in indoor environment: composition, health effects and analysis. Indian J Med Microbiol. 26(4):302. http://www.ijmm.org/text.asp?2008/26/4/302/43555.
   PubMed Web of Science ®Google Scholar
• Tang C-S, Wan G-H. 2013. Air quality monitoring of the post-operative recovery room and locations surrounding operating theaters in a medical center in Taiwan. Conly J, editor. PLoS One. 3 8(4):e61093. doi:10.1371/journal.pone.0061093.
   PubMed Web of Science ®Google Scholar
• Tangni EK, Pussemier L. 2007. Ergosterol and mycotoxins in grain dusts from fourteen Belgian cereal storages: A preliminary screening survey. J Sci Food Agric. 87(7):1263–1270. doi:10.1002/jsfa.2838.
   Web of Science ®Google Scholar
• Täubel M, Rintala H, Pitkäranta M, Paulin L, Laitinen S, Pekkanen J, Hyvärinen A, Nevalainen A. 2009. The occupant as a source of house dust bacteria. J Allergy Clin Immunol. 124(4):834–840.e47. http://linkinghub.elsevier.com/retrieve/pii/S0091674909011543.
   PubMed Web of Science ®Google Scholar
• Varga J, Baranyi N, Chandrasekaran M, Vágvölgyi C, Kocsubé S. 2015. Mycotoxin producers in the Aspergillus genus: an update. Acta Biol Szeged. 59:151–167.
   Google Scholar
• Viegas C. 2018. ISBN: 978-1- 138-54203-7. Sampling methods for an accurate mycobiota occupational exposure assessment – overview of several ongoing projects. In Occupational Safety and Hygiene VI Arezes et al. (Eds.). London: CRC Press. Taylor and Francis Group. 7–11.
   Google Scholar
• Viegas C, Faria T, Caetano LA, Carolino E, Gomes AQ, Viegas S. 2017. Aspergillus spp. prevalence in different Portuguese occupational environments: what is the real scenario in high load settings? J Occup Environ Hyg. 14(10):771–785.
   PubMed Web of Science ®Google Scholar
• Viegas C, Faria T, Monteiro A, Aranha Caetano L, Carolino E, Quintal Gomes A, Viegas S. 2018a. A novel multi-approach protocol for the characterization of occupational exposure to organic dust—swine production case study. Toxics. 6:5. doi:10.3390/toxics6010005
   Web of Science ®Google Scholar
• Viegas C, Malta-Vacas J, Sabino R 2012. Molecular biology versus conventional methods—complementary methodologies to understand occupational exposure to fungi. International Symposium on Occupational Safety and Hygiene SHO. Guimarães, Portugal.
   Google Scholar
• Viegas C, Monteiro A, Aranha Laetano L, Faria T, Carolino E, Viegas S. 2018b. Electrostatic dust cloth: A passive screening method to assess occupational exposure to organic dust in bakeries. Atmosphere. 9(2):1–14. doi:10.3390/atmos9020064.
   Web of Science ®Google Scholar
• Viegas C, Monteiro A, Dos Santos M, Faria T, Aranha CL, Carolino E, Quintal Gomes A, Marchand G, Lacombe N, Viegas S. 2018c. Filters from taxis air conditioning system: A tool to characterize driver’s occupational exposure to bioburden? Environ Res. 164:522–529. doi:10.1016/j.envres.2018.03.032.
   PubMed Web of Science ®Google Scholar
• Viegas C, Nurme J, Pieckova E, Viegas S. 2018d. Sterigmatocystin in foodstuffs and feed: aspects to consider. Mycology. doi:10.1080/21501203.2018.1492980
   PubMedGoogle Scholar
• Viegas C, Ramos C, Almeida M, Sabino R, Veríssimo C, Rosado L. 2011. Air fungal contamination in ten hospitals’ food units from Lisbon. In: WIT Transactions on Ecology and the Environment. p. 127–132. http://library.witpress.com/viewpaper.asp?pcode=FENV11-013-1.
   Google Scholar
• Viegas S, Assunção R, Nunes C, Osteresch B, Twaruzek M, Kosicki R, Grajewski J, Martins C, Alvito P, Almeida A, et al. 2018e. Exposure assessment to mycotoxins in a portuguese fresh bread dough company by using a multi-biomarker approach. Toxins. 10. p. 342. doi:10.3390/toxins10090342.
   Web of Science ®Google Scholar
• World Health Organization (WH). 2016. Antimicrobial resistance: fact sheet no 194. Genebra: WHO. http://www.who.int/mediacentre/factsheets/fs194/en/.
   Google Scholar
• World Health Organization (WHO). 2002. Prevention of hospital-acquired infections A PRACTICAL GUIDE. 2nd. France: WHO/CDS/CSR/EPH/2002.12. 72. http://www.who.int/emc%0Ahttp://apps.who.int/medicinedocs/documents/s16355e/s16355e.pdf.
   Google Scholar