miR-145-5p exerts anti-tumor effects in diffuse large B-cell lymphoma by regulating S1PR1/STAT3/AKT pathway

Abstract

To investigate the molecular mechanism of miR-145-5p in diffuse large B-cell lymphoma (DLBCL) tissues and cells. The tissues from patients with DLBCL were collected for RT-qPCR or immunohistochemistry. Cell viability, proliferation, migration, invasion, the relationship between miR-145-5p and S1PR1, and proteins related pathway were detected using CCK-8, BrdU staining, Transwell assay, dual luciferase report assay, and western blotting, respectively. The results showed that miR-145-5p was down-regulated and positively correlated with the survival of DLBCL patients. Overexpression of miR-145-5p inhibited cell proliferation, migration, and invasion in cell model. miR-145-5p directly targeted S1PR1. miR-145-5p down-regulated S1PR1, p-AKT/AKT, and p-STAT3 expression. The reduction of miR-145-5p-induced cell movement was reversed by S1PR1 overexpression. Moreover, S1PR1-induced addition of cell growth was clearly alleviated in LY294002 or S3I-201 treated cells. S1PR1 was up-regulated in the tissues of DLBCL patients. In conclusion, miR-145-5p regulated DLBCL cell growth and movement through suppressing S1PR1/STAT3/AKT pathway.

Keywords:

• miR-145-5p
• S1PR1
• AKT
• STAT3
• diffuse large B-cell lymphoma

Disclosure statement

The authors state that there are no conflicts of interest to disclose.

Data availability statement

All data generated or analyzed during this study are included in this published article.