A DNA barcode survey of marine macroalgae from Bergen (Norway)

ABSTRACT

Safeguarding the services provided by marine forests inherently depends on an accurate understanding of macroalgal species diversity. Here, we provide the first DNA barcode survey of marine macroalgae from Norway, with a focus on the Bergen area, and compared our findings to morphological listings for the corresponding location. Specimens were sampled 14–20 April and 3–13 June 2016, and variously sequenced for several genetic markers, including the five prime end of the cytochrome c oxidase subunit I gene (COI-5P), elongation factor tufA, and the ribulose-1, 5-biphosphate carboxylase large subunit gene (rbcL). We generated 655 new barcode records for COI-5P, 11 for tufA, and 50 for rbcL, representing nine species of Chlorophyta, 51 species of Phaeophyceae, and 74 species of Rhodophyta. Sequence data confirmed the presence of 113 morphological species. A further 17 genetic groups indicated the presence of new species for the area, only six of which were linked to formally described species. The remaining four genetic records were uncertain in terms of morphological species assignment and relation to the local flora. We recommend further DNA barcoding surveys in the area, as only a third of the listed morphological species were genetically confirmed.

KEYWORDS:

• Seaweeds
• species diversity
• marine forests
• DNA
• Atlantic

Acknowledgements

We thank Tomas Sørlie, Caroline S. Armitage, Katrine Kongshavn, and the students of BIO309 in 2016 who helped with the collection of specimens. We also thank Tanya Moore for helping to generate molecular data.

Disclosure statement

No potential conflict of interest was reported by the authors.

ORCID

Trevor T. Bringloe http://orcid.org/0000-0002-5976-6700

Additional information

Funding

This project was funded by the administered through Polar Knowledge Canada, the Natural Sciences and Engineering Research Council of Canada through an NSERC Post-Graduate Scholarship to T.T. Bringloe and a Discovery Grant (170151-2013) to G.W. Saunders, the New Brunswick Innovation Foundation, and the University of Melbourne McKenzie Postdoctoral Fellowship program.