LncRNA SNHG16 Induces the SREBP2 to Promote Lipogenesis and Enhance the Progression of Pancreatic Cancer

Abstract

Aim: Blocking lipogenesis could significantly inhibit the progression of pancreatic cancer. Exploring the regulatory mechanisms of lipogenesis by lncRNA SNHG16 might be of great significance to control the development of pancreatic cancer. Methods: The proliferation, migration, invasion and lipogenesis were determined with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, wound healing, transwell and Oil Red O staining assays, respectively. The interactions among lncRNA SNHG16, miR-195 and SREBP2 were analyzed by dual luciferase reporter assays. Results: Both the knock down of lncRNA SNHG16 and SREBP2 and overexpression of miR-195 suppressed the proliferation, migration, invasion and lipogenesis in pancreatic cancer cells. LncRNA SNHG16 directly sponged miR-195 to modulate the lipogenesis via regulating the expression of SREBP2. Conclusion: LncRNA SNHG16 accelerated the development of pancreatic cancer and promoted lipogenesis via directly regulating miR-195/SREBP2 axis.

Keywords::

• lipogenesis
• lncRNA SNHG16
• migration and invasion
• miR-195
• pancreatic cancer
• SREBP2

Data availability statement

All data generated and analyzed during the study are available from Y Yu and the authors can reproduce the experiments on reasonable request.

Financial&competing interests disclosure

This work was supported by Project of Minhang Natural Science Foundation (2017MHZ38); Great Discipline Construction Project from the Medical System of Shanghai Minhang District (no. 2017MWDXK01); Talent Development Plan funded by The Fifth People’s Hospital of Shanghai, Fudan University (no. 2017WYRCJY01); Medical Key Discipline Project funded by The Fifth People’s Hospital of Shanghai, Fudan University (no. 2017WYZDZK11). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

Additional information

Funding

This work was supported by Project of Minhang Natural Science Foundation (2017MHZ38); Great Discipline Construction Project from the Medical System of Shanghai Minhang District (no. 2017MWDXK01); Talent Development Plan funded by The Fifth People’s Hospital of Shanghai, Fudan University (no. 2017WYRCJY01); Medical Key Discipline Project funded by The Fifth People’s Hospital of Shanghai, Fudan University (no. 2017WYZDZK11). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.