Separation and Determination of Low Abundant Flavonoids in Scutellaria Baicalensis Georgi by Micellar Electrokinetic Capillary Electrophoresis

Abstract

Flavonoids are secondary metabolites in many plants with diverse biological activities. A simple and rapid micellar electrokinetic capillary electrophoresis method was developed for simultaneous determination of three low abundant flavonoids (chrysin, 5,7,4′-trihydroxy-8-methoxyflavone, and skullcapflavone II) in Scutellaria baicalensis Georgi within 12 min. The calibration curves revealed a good linear relationship between the peak areas of analytes and their concentrations (11.38 to 436.67 mg/L). The standard deviations of the migration time of the three flavonoids were determined to be 0.64%, 0.83%, and 1.17%, and peak areas were 6.14%, 7.57%, and 8.33%. Recoveries ranged between 90.37 and 109.4%.

Keywords:

• 8-hydroxymethylflavone
• chrysin
• Micellar electrokinetic capillary electrophoresis
• oroxylin-A
• skullcapflavone II
• wogonin

This work was supported by grants from the Ministry of Education, Taiwan (EMRPD160151 to J.-S. Yu), Chang Gung Memorial Hospital, Taiwan (CMRPD140121 to Y.-L. Leu), and the Natural Science Foundation of Gansu (3ZS061-A25–088 to Yuming Dong), Gansu Province, China.

Notes

^aY and c stand for the peak area and the concentration (mg/L) of the analytes, respectively.

^b The detection limit was defined as the concentration where the signal-to-noise ratio is 3.

^c The limit of quantitation was defined as the concentration where the signal-to-noise ratio is 10.

^a Std., standard.

^b n = 3.