77
Views
9
CrossRef citations to date
0
Altmetric
ELECTROPHORESIS

Separation and Determination of Low Abundant Flavonoids in Scutellaria Baicalensis Georgi by Micellar Electrokinetic Capillary Electrophoresis

, , &
Pages 1444-1457 | Received 24 Oct 2008, Accepted 09 Apr 2009, Published online: 18 Jun 2009

REFERENCES

  • Anjia , C. , L. Cunhong , G. Wenhua , H. Zhide , and C. Xingguo . 2005 . Separation and determination of active components in Schisandra chinensis Baill. and its medicinal preparations by non-aqueous capillary electrophoresis . Biomed. Chromatogr. 19 : 481 – 487 .
  • Asztemborska , M. , M. Miśkiewicz , and D. Sybilska . 2003 . Separation of some chiral flavanones by micellar electrokinetic chromatography . Electrophoresis 24 : 2527 – 2531 .
  • Baylor , N. W. , T. Fu , Y. D. Yan , and F. W. Ruscetti . 1992 . Inhibition of human T cell leukemia virus by the plant flavonoid baicalin (7-glucuronic acid, 5,6-dihydroxyflavone) . J. Infect. Dis. 165 : 433 – 437 .
  • Chen , G. , H. W. Zhang , and J. N. Ye . 2000. Determination of baicalein, baicalin and quercetin in Scutellariae radix and its preparations by capillary electrophoresis with electrochemical detection. Talanta 53: 471–479.
  • China Pharmacopoeia Committee . 2004 . Pharmacopoeia of People's Republic of China. Beijing , China : China Chemical Industry Press.
  • Choi , J. , C. C. Conrad , C. A. Malakowsky , J. M. Talent , C. S. Yuan , and R. W. Gracy . 2002 . Flavones from Scutellaria baicalensis Georgi attenuate apoptosis and protein oxidation in neuronal cell lines . Biochim. Biophys. Acta 1571 : 201 – 210 .
  • Dong , Y. M. , W. D. Duan , Y. Wang , and S. Y. Duan . 2003 . Determination of baicalin in Kechuan Capsules by RP-HPLC . Chin. Trad. Patent Med. 25 : 775 – 776 .
  • Gao , Z. , K. Huang , X. Yang , and H. Xu . 1999 . Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi . Biochim. Biophys. Acta 1472 : 643 – 650 .
  • Huang , Y. , C. M. Wong , C. W. Lau , X. Yao , S. Y. Tsang , Y. L. Su , and Z. Y. Chen . 2004 . Inhibition of nitric oxide/cyclic GMP-mediated relaxation by purified flavonoids, baicalin and baicalein, in rat aortic rings . Biochem. Pharmacol. 67 : 787 – 794 .
  • Huang , Y. B. , P. C. Wu , C. S. Su , Y. C. Wu , and Y. H. Tsai . 2006 . Simultaneous quantification of twelve bioactive components in San-Huang-Xie-Xin-Tang by HPLC . Phytochem. Anal. 17 : 439 – 446 .
  • Hui , K. M. , M. S. Huen , H. Y. Wang , H. Zheng , E. Sigel , R. Baur , H. Ren , Z. W. Li , J. T. Wong , and H. Xue . 2002 . Anxiolytic effect of wogonin, a benzodiazepine receptor ligand isolated from Scutellaria baicalensis Georgi . Biochem. Pharmacol. 64 : 1415 – 1424 .
  • Krakauer , T. , B. Q. Li , and H. A. Young . 2001 . The flavonoid baicalin inhibits superantigen-induced inflammatory cytokines and chemokines . FEBS Lett. 500 : 52 – 55 .
  • Lee , H. , Y. O. Kim , H. Kim , S. Y. Kim , H. S. Noh , S. S. Kang , G. J. Cho , W. S. Choi , and K. Suk . 2003 . Flavonoid wogonin from medicinal herb is neuroprotective by inhibiting inflammatory activation of microglia . FASEB J. 17 : 1943 – 1944 .
  • Li , C. H. , J. Y. Zhang , A. J. Chen , J. P. Xie , X. G. Chen , and Z. D. Hu . 2005 . Non-aqueous capillary electrophoresis for simultaneous separation and determination of three major active components in traditional medicinal preparations . Biomed. Chromatogr. 19 : 369 – 374 .
  • Li , C. Y. , C. H. Chiu , H. S. Huang , C. H. Lin , and T. S. Wu . 2006 . High-performance liquid chromatographic method for simultaneous quantification of eight major biologically active ingredients in Da-Chai-Hu-Tang preparation . Biomed. Chromatogr. 20 : 305 – 308 .
  • Li , H. B. , Y. Jiang , and F. Chen . 2004 . Separation methods used for Scutellaria baicalensis active components . J. Chromatogr. B 812 : 277 – 290 .
  • Li , K. L. , and S. J. Sheu . 1995 . Determination of flavonoids and alkaloids in the scute-coptis herb couple by capillary electrophoresis . Anal. Chim. Acta. 313 : 113 – 120 .
  • Liu , J. J. , T. S. Huang , W. F. Cheng , and F. J. Lu . 2003. Baicalein and baicalin are potent inhibitors of angiogenesis: Inhibition of endothelial cell proliferation, migration, and differentiation. Int. J. Cancer. 106: 559–565.
  • Liu , Y. M. , and S. J. Sheu . 1994 . Capillary electrophoresis determination of baicalein, baicalin, wogonin, and wogonin-7-O-glucuronide in traditional Chinese medicinal preparation . J. High Resolut. Chromatogr. 17 : 559 – 560 .
  • Morina , P. , J. C. Archambaultb , P. Andre , M. Dreuxa , and E. Gaydouc . 1997 . Separation of hydroxylated and methoxylated flavonoids by micellar electrokinetic capillary chromatography determination of analyte partition coefficients between aqueous and sodium dodecyl sulfate micellar phases . J. Chromatogr. A 791 : 289 – 297 .
  • Nan , J. X. , E. J. Park , Y. C. Kim , G. Ko , and D. H. Sohn . 2002 . Scutellaria baicalensis inhibits liver fibrosis induced by bile duct ligation or carbon tetrachloride in rats . J. Pharm. Pharmacol. 54 : 555 – 563 .
  • Ng , C. L. , C. P. Ony , M. K. Lee , and S. F. Y. Li . 1992 . Systematic optimization of micellar electrokinetic chromatographic separation of flavonoids . Chromatographia 34 : 166 – 172 .
  • Ng , C. L. , M. K. Lee , and S. F. Y. Li . 1992 . Systematic optimization of capillary electrophoretic separation of sulphonamides . J. Chromatogr. A 598 : 133 – 138 .
  • Shao , Z. H. , C. Q. Li , T. L. VandenHoek , L. B. Becker , P. T. Schumacker , J. A. Wu , A. S. Attele , and C. S. Yuan . 1999 . Extract from Scutellaria baicalensis Georgi attenuates oxidant stress in cardiomyocytes . J. Mol. Cell Cardiol. 31 : 1885 – 1895 .
  • Tang , W. , X. Sun , J. S. Fang , M. Zhang , and N. J. Sucher . 2004 . Flavonoids from Radix scutellariae as potential stroke therapeutic agents by targeting the second postsynaptic density 95 (PSD-95)/disc large/zonula occludens-1 (PDZ) domain of PSD-95 . Phytomedicine 11 : 277 – 284 .
  • Terabe , S. 1992 . Selectivity manipulation in micellar electrokinetic chromatography . J. Pharm. Biomed. Anal. 10 : 705 – 715 .
  • Terabe , S. , K. Otsuka , and T. Ando . 1985 . Electrokinetic chromatography with micellar solution and open-tubular capillary . Anal. Chem. 57 : 834 – 841 .
  • Wang , S. P. , M. D. Fu , and M. H. Wang . 2007 . Separation mechanism and determination of flavanones with capillary electrophoresis and high-performance liquid chromatography . J. Chromatogr. A 1164 : 306 – 312 .
  • Yu , K. , Y. F. Gong , Z. Y. Lin , and Y. Y. Cheng . 2007 . Quantitative analysis and chromatographic fingerprinting for the quality evaluation of Scutellaria baicalensis Georgi using capillary electrophoresis . J. Pharm. Biomed. Anal. 43 : 540 – 548 .
  • Zhang , H. G. , K. Tian , J. H. Tang , S. D. Qi , H. L. Chen , X. G. Chen , and Z.D. Hu . 2006 . Analysis of baicalein, baicalin, and wogonin in Scutellariae radix and its preparation by microemulsion electrokinetic chromatography with 1-butyl-3-methylimizolium tetrafluoborate ionic liquid as additive . J. Chromatogr. A 1129 : 304 – 307 .
  • Zhou , L. , S. M. Wang , K. Tian , Y. M. Dong , and Z. D. Hu . 2007 . Simultaneous determination of catecholamines and amino acids by micellar electrokinetic chromatography with laser induced fluorescence detection . J. Sep. Sci. 30 : 110 – 117 .

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.