A Novel HPLC-PDA-MS Method for S-Adenosylmethionine and S-Adenosylhomocysteine Routine Analysis

Abstract

S-adenosylmethionine and S-adenosylhomocysteine are essential compounds in metabolic pathways; therefore, the development of a suitable method to routinely quantify these compounds is very important. In this paper, a simple and fast method (10 minutes) for simultaneous SAM and SAH quantification in plasma and blood samples is proposed based on the HPLC-PDA-MS technique. A new solid phase extraction procedure for blood samples is reported. The developed method proved to be highly sensitive (1.3 × 10⁻⁹ molL⁻¹ for SAM and 4.5 × 10⁻¹⁰ molL⁻¹ for SAH), repeatable (CV = 1.06% for SAM and CV = 0.92% for SAH), and reproducible (CV = 4.1% for SAM and CV = 3.77% for SAH).

Keywords:

• HPLC
• MS
• S-adenosylhomocysteine
• S-adenosylmethionine
• SPE

The authors are grateful to Romanian Ministry of Education and Research (research grant PNCDII 62-057/2008 (AGECVD) for the financial support.