Completing the genetic puzzle of the reed warbler complex: insights from Italy

Figures & data

Table 1. Sampling details of reed warbler specimens collected from Italy for this study. The identifier of sampling sites in the map of Figure 1, the cyt b haplotypes and the GenBank accession numbers of the sequenced samples are also reported (* haplotypes discovered in this study).

Individual Id	Haplotype	Sampling Area	Map Id	Sample Site Name	Latitude	Longitude	Accession Number
IT 0985	ACR1	Southern Italy	2	Pignola	40.5838	15.7486	LR878457
IT 0887	ACR1	Sardinia	3	Terralba	39.7099	8.5506	LR878456
IT 1280	ACR9	Sicily	4	Augusta	37.2072	15.1810	LR878458
AJAH222	ACR10	Northern Italy	1	Fontanetto Po	45.2193	8.1772	LR878459
AJAH228	ACR10	Northern Italy	1	Fontanetto Po	45.2193	8.1772	LR878460
AJAH289	ACR10	Northern Italy	1	Fontanetto Po	45.2193	8.1772	LR878461
IT 0897	ACR10	Sardinia	3	Terralba	39.7099	8.5506	LR878462
IT 0903	ACR10	Sardinia	3	Terralba	39.7099	8.5506	LR878463
IT 1322	ACR10	Sicily	5	Ispica	36.7166	15.0175	LR878464
IT 0902	ACR67*	Sardinia	3	Terralba	39.7099	8.5506	LR878465
IT 0984	ACR68*	Southern Italy	2	Pignola	40.5838	15.7486	LR878466
IT 0987	ACR69*	Southern Italy	2	Pignola	40.5838	15.7486	LR878467
IT 1281	ACR70*	Sicily	4	Augusta	37.2072	15.1810	LR878468
IT 1321	ACR71*	Sicily	5	Ispica	36.7166	15.0175	LR878469
IT 0890	ACR72*	Sardinia	3	Terralba	39.7099	8.5506	LR878470
IT 0983	ACR72*	Sardinia	3	Terralba	39.7099	8.5506	LR878471
IT 0984	ACR73*	Sardinia	3	Terralba	39.7099	8.5506	LR878472

Figure 1. Median-joining network of cyt b haplotypes of Western Palearctic Acrocephalus scirpaceus/A. baeticatus species complex populations (Table 1, Table S1). Each circle represents a haplotype and circle size is proportional to haplotype frequency. Colours indicate different subspecies and blue dots also account for Italian sampling sites. Dashes represent substitutions. Distribution map is shown on the countries boundaries basis.

Table 2. Between groups genetic divergence values of cyt b sequences, based on the uncorrected p-distance calculation (bottom left), for the taxa of European and African Reed Warblers considered in this study. Standard error values are reported above the diagonal.

	A. b. ambiguus	A. b. baeticatus	A. b. minor	A. b. hallae	A. s. avicenniae	A. s. fuscus	A. s. scirpaceus	Italian populations
A. b. ambiguus		0.0039	0.0034	0.0052	0.0032	0.0045	0.0042	0.0045
A. b. baeticatus	0.0144		0.0025	0.0031	0.0035	0.0049	0.0045	0.0047
A. b. minor	0.0091	0.0091		0.0051	0.0036	0.0050	0.0044	0.0047
A. b. hallae	0.0198	0.0115	0.0186		0.0041	0.0049	0.0050	0.0052
A. s. avicenniae	0.0112	0.0163	0.0120	0.0205		0.0028	0.0028	0.0033
A. s. fuscus	0.0185	0.0229	0.0193	0.0254	0.0131		0.0036	0.0039
A. s. scirpaceus	0.0171	0.0222	0.0180	0.0252	0.0116	0.0171		0.0011
Italian populations	0.0178	0.0230	0.0187	0.0261	0.0122	0.0177	0.0039