Figures & data
Figure 1. Experimental materials and treatments. (A) Fruit bag, (B) a bagged Pyrus pyrifolia ‘Cuiguan’ fruit, (C) previously bagged ‘Cuiguan’ fruit and (D) unbagged ‘Cuiguan’ fruit (control).
![Figure 1. Experimental materials and treatments. (A) Fruit bag, (B) a bagged Pyrus pyrifolia ‘Cuiguan’ fruit, (C) previously bagged ‘Cuiguan’ fruit and (D) unbagged ‘Cuiguan’ fruit (control).](/cms/asset/23bfb19c-3fd0-44ef-bc46-41ca44e0d9d7/tnzc_a_1462209_f0001_oc.jpg)
Figure 2. Effect of bagging on the L-AsA/DHA couple during development of Pyrus pyrifolia ‘Cuiguan’ fruit. (A) Ascorbic acid (AsA), (B) dehydroascorbate (DHA), (C) total AsA (AsA + DHA) and (D) AsA/DHA ratio. Results are means ± SD (n = 3); (●): unbagged fruit; (○): bagged fruit. ns = not significant (p < .05); * and **, significant at p < .05 and p < .01, respectively, Duncan multiple-range test. FW: fresh weight.
![Figure 2. Effect of bagging on the L-AsA/DHA couple during development of Pyrus pyrifolia ‘Cuiguan’ fruit. (A) Ascorbic acid (AsA), (B) dehydroascorbate (DHA), (C) total AsA (AsA + DHA) and (D) AsA/DHA ratio. Results are means ± SD (n = 3); (●): unbagged fruit; (○): bagged fruit. ns = not significant (p < .05); * and **, significant at p < .05 and p < .01, respectively, Duncan multiple-range test. FW: fresh weight.](/cms/asset/060df3ef-5746-49b0-ac9c-9f2c92869f26/tnzc_a_1462209_f0002_ob.jpg)
Figure 3. Effect of bagging on the GSH/GSSG couple during development of Pyrus pyrifolia ‘Cuiguan’ fruit. (A) Oxidised glutathione (GSSG) and (B) total GSH [reduced glutathione (GSH) + GSSG]. GSH was measured from the same tissues in A. Results are means ± SD (n = 3); (●): unbagged fruit; (○): bagged fruit. ns = not significant (p < .05), Duncan multiple-range test. FW: fresh weight.
![Figure 3. Effect of bagging on the GSH/GSSG couple during development of Pyrus pyrifolia ‘Cuiguan’ fruit. (A) Oxidised glutathione (GSSG) and (B) total GSH [reduced glutathione (GSH) + GSSG]. GSH was measured from the same tissues in A. Results are means ± SD (n = 3); (●): unbagged fruit; (○): bagged fruit. ns = not significant (p < .05), Duncan multiple-range test. FW: fresh weight.](/cms/asset/fd53eb78-0279-4e64-aad4-9ebdd6dafdeb/tnzc_a_1462209_f0003_ob.jpg)
Figure 4. Effect of bagging on enzymes in L-galactose pathway during development of Pyrus pyrifolia ‘Cuiguan’ fruit. (A) L-galactono-1,4-lactone dehydrogenase (GLDH) activity and (B) L-galactose dehydrogenase (GalDH) activity. Results are means ± SD (n = 3); (●): unbagged fruit; (○): bagged fruit. ns = not significant (p < .05); * and **, significant at p < .05 and p < .01, respectively, Duncan multiple-range test. FW: fresh weight.
![Figure 4. Effect of bagging on enzymes in L-galactose pathway during development of Pyrus pyrifolia ‘Cuiguan’ fruit. (A) L-galactono-1,4-lactone dehydrogenase (GLDH) activity and (B) L-galactose dehydrogenase (GalDH) activity. Results are means ± SD (n = 3); (●): unbagged fruit; (○): bagged fruit. ns = not significant (p < .05); * and **, significant at p < .05 and p < .01, respectively, Duncan multiple-range test. FW: fresh weight.](/cms/asset/6be376f1-15f5-4659-9e66-0de34c1b9c3b/tnzc_a_1462209_f0004_ob.jpg)
Figure 5. Effect of bagging on enzymes in the ascorbate-glutathione cycle during development of Pyrus pyrifolia ‘Cuiguan’ fruit. (A) Ascorbate peroxidase (APX) activity, (B) monodehydroascorbate reductase (MDHAR) activity, (C) dehydroascorbate reductase (DHAR) activity and (D) glutathione reductase (GR) activity. Results are means ± SD (n = 3); (●): unbagged fruit; (○): bagged fruit. ns = not significant (p < .05); * and **, significant at p < .05 and p < .01, respectively, Duncan multiple-range test. FW: fresh weight.
![Figure 5. Effect of bagging on enzymes in the ascorbate-glutathione cycle during development of Pyrus pyrifolia ‘Cuiguan’ fruit. (A) Ascorbate peroxidase (APX) activity, (B) monodehydroascorbate reductase (MDHAR) activity, (C) dehydroascorbate reductase (DHAR) activity and (D) glutathione reductase (GR) activity. Results are means ± SD (n = 3); (●): unbagged fruit; (○): bagged fruit. ns = not significant (p < .05); * and **, significant at p < .05 and p < .01, respectively, Duncan multiple-range test. FW: fresh weight.](/cms/asset/3fe4ff44-c64a-4a7a-90e5-70c061a5a5ca/tnzc_a_1462209_f0005_ob.jpg)
Figure 6. Effect of bagging on the expression levels of genes coding for L-galactose dehydrogenase (GalDH), L-galactono-1,4-lactone dehydrogenase (GLDH), monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) during development of Pyrus pyrifolia ‘Cuiguan’ fruit. Results are means ± SD (n = 3) for unbagged fruit (back column) and bagged fruit (white column). ns = not significant (p < .05); * and **, significant at p < .05 and p < .01, respectively, Duncan multiple-range test.
![Figure 6. Effect of bagging on the expression levels of genes coding for L-galactose dehydrogenase (GalDH), L-galactono-1,4-lactone dehydrogenase (GLDH), monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) during development of Pyrus pyrifolia ‘Cuiguan’ fruit. Results are means ± SD (n = 3) for unbagged fruit (back column) and bagged fruit (white column). ns = not significant (p < .05); * and **, significant at p < .05 and p < .01, respectively, Duncan multiple-range test.](/cms/asset/968ce013-a20c-4c55-893b-b1a6b4d358b8/tnzc_a_1462209_f0006_ob.jpg)