MTHFR and MTRR gene polymorphisms in patients with chronic hepatitis B virus infections in Zigong, Sichuan Province

Figures & data

Figure 1. Schematic diagram of PCR-melt curve method.

Table 1. Hardy-Weinberg equilibrium test for analysing MTHFR and MTRR gene polymorphisms in patients with chronic HBV infection from Zigong, n (%).

Locus	Genotype	Actual frequency	Theoretical frequency	χ^2	p
MTHFR C677T	CC	79 (41.36)	83 (43.46)	0.592	0.744
	CT	93 (48.69)	86 (45.03)
	TT	19 (9.95)	22 (11.52)
MTRR A66G	AA	115 (60.21)	117 (61.26)	0.387	0.824
	AG	69 (36.13)	65 (34.03)
	GG	7 (3.66)	9 (4.71)
MTHFR A1298C	AA	136 (71.20)	135 (70.68)	0.014	0.993
	AC	50 (26.18)	51 (26.70)
	CC	5 (2.62)	5 (2.62)

Table 2. Analysis of genetic polymorphisms of MTHFR and MTRR in patients with chronic HBV infection from Zigong.

Locus	Total	Genotype	Cases	Percentage (%)
MTHFR C677T	191	CC	79	41.36%
		CT	93	48.69%
		TT	19	9.95%
MTRR A66G	191	AA	115	60.21%
		AG	69	36.13%
		GG	7	3.66%
MTHFR A1298C	191	AA	136	71.20%
		AC	50	26.18%
		CC	5	2.62%

Table 3. Comparative analysis of genetic polymorphisms of MTHFR and MTRR in patients with chronic HBV infection stratified by age, n (%).

	Genotype	≤ 60 years old (n = 106)	> 60 years old (n = 85)	χ^2/Fi	p
MTHFR C677T	CC	46 (43.4)	33 (38.8)	1.625*	0.444
	CT	52 (49.1)	41 (48.2)
	TT	8 (7.5)	11 (12.9)
MTRR A66G	AA	66 (62.3)	49 (57.6)	0.557^#	0.835
	AG	36 (34.0)	33 (38.8)
	GG	4 (3.8)	3 (3.5)
MTHFR A1298C	AA	81 (76.4)	55 (64.7)	3.671^#	0.155
	AC	22 (20.8)	28 (32.9)
	CC	3 (2.8)	2 (2.4)

* χ²; ^# Fi.

Table 4. Comparative analysis of genetic polymorphisms of MTHFR and MTRR in patients with chronic HBV infection stratified by gender, n (%).

	Genotype	Male (n = 136)	Female (n = 55)	χ^2/Fi	p
MTHFR C677T	CC	56 (41.2)	23 (41.8)	0.770*	0.714
	CT	68 (50.0)	25 (45.5)
	TT	12 (8.8)	7 (12.7)
MTRR A66G	AA	79 (58.1)	36 (65.5)	2.249^#	0.353
	AG	53 (39.0)	16 (29.1)
	GG	4 (2.9)	3 (5.5)
MTHFR A1298C	AA	96 (70.6)	40 (72.7)	0.176^#	1.000
	AC	36 (26.5)	14 (25.5)
	CC	4 (2.9)	1 (1.8)

* χ²; ^# Fi.

Table 5. Comparative analysis of genetic polymorphisms of MTHFR and MTRR in different groups of patients [n(%)].

	Genotype	CHB (n = 71)	LC (n = 56)	PLC (n = 64)	CHB vs. LC	CHB vs. PLC	PLC vs. LC
MTHFR C677T	CC	31 (43.7)	24 (42.9)	25 (37.9)	χ^2=0.239 p = 0.888	χ^2=0.882 p = 0.661	χ^2=0.326 p = 0.926
	CT	32 (45.1)	27 (48.2)	35 (53.0)
	TT	8 (11.3)	5 (8.9)	6 (9.1)
MTRR A66G	AA	42 (59.2)	31 (55.4)	42 (65.6)	Fi = 0.331 p = 0.955	Fi = 1.114 p = 0.645	Fi = 2.092 p = 0.355
	AG	26 (36.6)	22 (39.3)	21 (32.8)
	GG	3 (4.2)	3 (5.4)	1 (1.6)
MTHFR A1298C	AA	50 (70.4)	43 (76.8)	43 (67.2)	Fi = 3.510 p = 0.12	Fi = 3.015 p = 0.263	Fi = 1.412 p = 0.550
	AC	21 (29.6)	11 (19.6)	18 (28.1)
	CC	0 (0)	2 (3.6)	3 (4.7)

Availability of data and materials

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.