Identification of mitophagy and ferroptosis-related hub genes associated with intracerebral haemorrhage through bioinformatics analysis

Figures & data

Figure 1. (A) Identification of DEGs. (B) Identification of DEMFRGs. (C) GO enrichment analysis of DEMFRGs. (D) KEGG pathway enrichment analysis of DEMFRGs.

Figure 2. (A) PPI network of DEMFRGs. (B) PPI network of the 10 hub genes identified by the MCODE algorithm. (C) PPI network of the 10 hub genes identified by the MCC algorithm. (D) Heatmap of gene expression for the 10 hub genes in the Healthy group and ICH group. (E) Boxplot of gene expression for the 10 hub genes in the Healthy group and ICH group. *p < .05; **p < .01; ***p < .001. (F) Correlation heatmap of the 10 hub genes.

Figure 3. (A) ROC curves of the 10 hub genes in the GSE24265 dataset. (B) ROC curves of the 10 hub genes in the GSE149317 dataset.

Figure 4. miRNA-hub gene interaction network. Green triangles represent hub genes; orange dots represent miRNAs.

Figure 5. (A–B): Differential analysis of immune microenvironment based on ssGSEA algorithm. (C): Differential analysis of immune microenvironment based on CIBERSORT algorithm. (D): Differential analysis of immune microenvironment based on MCP algorithm. (E): Differential analysis of immune microenvironment based on xCell algorithm. *p < .05; **p < .01; ns: p > .05.

Table 1. ICH-targeted small molecule drugs predicted through cMAP database.

Score	Type	ID	Name	Description
−99.93	cp	BRD-A01145011	zebularine	DNA methyltransferase inhibitor
−99.93	cp	BRD-K94176593	TWS-119	Glycogen synthase kinase inhibitor
−99.89	cp	BRD-K84085265	CG-930	JNK inhibitor
−99.89	cp	BRD-K21971034	OM-137	Aurora kinase inhibitor
−99.89	cp	BRD-K63195589	tipifarnib	Farnesyltransferase inhibitor
−99.89	cp	BRD-A24514565	warfarin	Vitamin K antagonist
−99.86	cp	BRD-K54330070	SB-202190	p38 MAPK inhibitor
−99.86	cp	BRD-K44084986	Y-27632	Rho associated kinase inhibitor
−99.86	cp	BRD-K62810658	PD-98059	MEK inhibitor
−99.82	cp	BRD-K01638814	rilmenidine	Adrenergic receptor agonist

Data availability statement

The data and materials in the current study are available from the corresponding author on reasonable request.