Figures & data
Table 1. Mitogenic response of splenocytes of 4-week-old SPF chickens (n=28) stimulated with different concentrations of several mitogens and ChIL-2
Figure 1. Optimization of the type of recall antigen (at 1 μg/ml) and the time of the harvest for antigen-specific ChIFN-γ production of splenocytes from immune and negative chickens. Splenocytes were stimulated with a combination of PMA/ionomycin (PMA/Iono, 0.1 μg/ml), recombinant ChIL-2 (rChIL2, 10 μg/ml), inactivated and purified NDV (inactivated NDV), dissociated NDV proteins (All prot. NDV) or purified NDV envelope glycoproteins (gpNDV), and supernatants of stimulated splenocytes were harvested after 48 or 72 h of activation. ChIFN-γ production was determined by the ChIFN-γ capture ELISA. O.D., optical density.
Figure 2. Determination of the optimal concentration of antigen for the specific recall stimulation. Splenocytes were stimulated with dissociated NDV proteins (All prot. NDV) or purified NDV glycoproteins (gpNDV) used at different concentrations (μg/ml), and supernatants of stimulated splenocytes were harvested after 72 h of activation. O.D., optical density.
