Abstract
A series of 4-hydroxy-3-methoxy benzaldehyde (vanillin) derivatives (3a–3r) was designed for the principle of Schiff base condensation with several individual sulfanilamide analogues. The inhibitory potencies of the designed compounds were evaluated through molecular docking simulation studies against the targets, breast cancer-topo isomerase-IIα and estrogen receptor-α; and the top scoring poses with higher binding energy were selected to assess the mode of binding and stability of each complex through molecular dynamics simulations. Compounds that remained stable in the active sites of the both target receptors through a number of strong H-bonds and hydrophobic contacts were selected. Based on the computational results, these selected compounds, 3b, 3e and 3f were synthesized and were followed up for structural elucidation attempts, by FT/ATR, 1H NMR and 13C NMR. From the experimental in vitro studies on 3b, 3e and 3f, the following remarkable activities against breast cancer cell line were done; IC50 values of 3b, 3e and 3f were noted, 6.7, 4.3 and 11 ng/mL, respectively. These newly synthesized compounds may be used as novel inhibitors of nuclear receptors with potential therapeutic applications in control of cancer.
Communicated by Ramaswamy H. Sarma
Acknowledgments
Authors are thankful to the Department of Chemistry, Ravenshaw University, Cuttack for 1H, 13C-spectral facilities; Department of Biotechnology, Mizoram Central University, Aizawl; and we gratefully acknowledge SPS, IMS & SUM Hospital of Siksha O Anusandhan Deemed to be University, Bhubaneswar-3, for necessary facilities.
Disclosure statement
The authors declared no conflict of interest.