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Food & Nutrition Science

Kinetic study of the quenching reaction of singlet oxygen by seven rice bran extracts in ethanol solution. Development of a singlet oxygen absorption capacity (SOAC) assay method

, , , , , , & show all
Pages 2063-2072 | Received 29 May 2015, Accepted 25 Jun 2015, Published online: 29 Jul 2015

Figures & data

Table 1. and values for rice bran extracts 1–7 in ethanol at 35.0 °C, relative rate constants , and relative SOAC Values.

Fig. 1. Measurement of the amounts of carotenoids contained in Nipponbare.

Notes: Typical UV chromatograms of (A) three carotenoid standards and (B) Nipponbare (rice bran extract 3).
Fig. 1. Measurement of the amounts of carotenoids contained in Nipponbare.

Table 2. Amounts of three carotenoids contained in rice bran extracts 1–7.

Fig. 2. Measurement of the second-order rate constant (kQ) for the reaction of Wataribune with singlet oxygen.

Notes: (A) Change in the absorbance of DPBF at 411 nm during the reaction of DPBF with 1O2 in the absence and presence of AOs (α-tocopherol and Wataribune (rice bran extract 2)) in ethanol at 35 °C. [DPBF] = 6.29 × 10−5 M and [EP] = 8.12 × 10−4 M. (B) Change in the absorbance of DPBF, where the correction of baseline due to Wataribune was performed (see text). (C) Plot of ln (Absorbance) vs. time. (D) Plot of SBlank/SWataribune vs. [Wataribune]. (E) Plot of t1/2Wataribune/t1/2Blank vs. [Wataribune].
Fig. 2. Measurement of the second-order rate constant (kQ) for the reaction of Wataribune with singlet oxygen.
short-legendScheme 1.

Table 3. Comparison between observed and calculated 1O2-quenching rates ( (Obsd.) and for Koshihikari (6) in ethanol solution.

Table 4. Comparison between observed and calculated 1O2-quenching rates ( (Obsd.) and (Calcd.) (See Text)) for rice bran extracts 1–7 in ethanol, ratio of the rate constants ( (Obsd.)/ (Calcd.)), and contributions of (i) 4 Tocs and 4 Toc-3s, (ii) 3 Cars, and (iii) γ-Ory.

Supplemental material

Supporting_Information__Table_S1_and_Figure_S1_.docx

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