Vitamin B₁₂ deficiency-induced increase of osteoclastic bone resorption caused by abnormal renal resorption of inorganic phosphorus via Napi2a

Figures & data

Fig. 1. Vitamin B₁₂ deficiency affects bone morphology.

Notes: The effect of bone morphology was measured using OsteoMeasure (A) Osteoclast Numbers/Bone Surface (N. Oc/BS), (B) Bone Volume/Tissue Volume (BV/TV), and (C) the trabecular separation shown in vitamin B₁₂ deficiency and supplementations. Values are means ± SD, control and vitamin B₁₂ deficient; n = 6, supplementation; n = 3 per group. Bars with different superscript letters indicate significant differences among groups with p < 0.05.

Fig. 2. Serum Pi levels change in vitamin B₁₂-deficient rats but not Ca and PTH levels.

Notes: Serum Pi, Ca, and PTH levels in vitamin B₁₂-deficient and supplemented rats are shown in (A), (B), and (C), respectively. Values are means ± SD. n = 4 per group. Bars with different superscript letters indicate significant differences among groups with p < 0.05.

Fig. 3. Napi2a translocation is inhibited during vitamin B₁₂ deficiency.

Notes: (A) Quantitative PCR analysis of Napi2a gene expression. Bar graphs show arbitrary units (means ± SD) of expression relative to β-actin expression. n = 4 per group. (B) Western blotting data of Napi2a in the kidney of vitamin B₁₂-deficient rats. (C) Quantification of intensity of (B) is shown. Bars with different superscript letters indicate significant differences among groups with p < 0.01. (D) The Napi2a immunostaining was performed using control, vitamin B₁₂ deficiency, and supplementation. (E) Schematic model of this study.