Figures & data
Fig. 1. Weight loss by EW-diet-feeding was rescued by OVA-feeding after sensitization in a food allergy model.
Notes: (a) experimental schedules for sensitization and administration of OVA in a food allergic model. BALB/c mice were sensitized by two i.p injections of OVA + alum. Seven days after the second immunization, mice were administrated OVA orally seven times every other day for two weeks. From the next day of the last administration, mice were fed with EW-diet. (b) Weight change was measured from day 0 (upper panel) and day14 (lower panel). *p < 0.05, showing results of statistical analysis between the OVA and the saline group. †p < 0.05, ††p < 0.01, showing results of statistical analysis of weight change during the course of EW-diet-feeding.
Fig. 2. Cytokine response, IgE antibody levels and intestinal histology analysis of OVA-administrated mice.
Notes: (a) CD4+ T cells of SPL and MLN were incubated with antigen-presenting cells and OVA. IL-2, IL-4, and IFN-γ in culture supernatants were measured by ELISA. Data are shown as mean ± SD of cultured wells. (b) Serum IgE titer were measured by ELISA. (c) Jejunal histologic analyses by hematoxylin and eosin stain. These data represent 2–3 independent experiments. *p < 0.05, **p < 0.01, #, not detected.
Fig. 3. Effects of oral administration of OVA on the cytokine response, IgE titer and intestinal inflammation after EW-diet-feeding.
Notes: (a) CD4+ T cells of SPL and MLN were incubated with antigen-presenting cells and OVA. IL-2, IL-4, and IFN-γ in culture supernatants were measured by ELISA. Data are shown as mean ± SD of cultured wells. (b) Serum IgE titer were measured by ELISA. (c) Jejunal histologic analyses by hematoxylin and eosin stain. (d) The ratio of villous height to crypt depth was analyzed. These data represent 2–3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, #, not detected.
Supplemental material
