Figures & data
Figure 1. Chemical structures of tea catechins and theaflavins.
Figure 3. Precipitation of PC after incubation with tea polyphenols (A: ECg, B: EGCg, C: TF1, D: TF2A, E: TF2B, and F: TF3) at 37 °C (left), and their redispersion by subsequent addition of taurocholate (right). Data are means ± SD (n = 3). *Significantly lower than control (p < 0.05).
Figure 4. Effects of tea polyphenols on taurocholate-PC micelles. (A) Changes in turbidity of the micelle solution during incubation with tea polyphenols at 37 °C. Data for ECg (open circles), EGCg (closed circles), TF1 (open triangles), TF2A (closed triangles), TF2B (open squares), TF3 (closed squares), and control (x marks) are means ± SD (n = 3). (B) Distribution of tea polyphenols in the Sup (open bars) and Ppt (closed bars) fractions after a 60-min incubation (nd: not detected). Data are means ± SD (n = 3).
Figure 5. Effects of phospholipid composition on the interaction between TF2A and bile salt-PC micelles. (A) Changes in turbidity of the micelle solution during incubation with 0.5 mM TF2A at 37 °C. Data for PC micelles (open circles), PC:PE (closed circles), and PC:PS (open triangles) are means ± SD (n = 3). (B) Distribution of TF2A in the Sup (open bars) and Ppt (closed bars) fractions after a 60-min incubation. Data are means ± SD (n = 3). *Significantly different from PC (p < 0.05).
Figure 6. Effects of PC concentration on the interaction between TF2A and bile salt-PC micelles. (A) PC-dependent elevation of turbidity of micelle solutions incubated with 0.5 mM TF2A for 60 min at 37 °C. Data are means ± SD (n = 3). (B) Distribution of TF2A in the Sup (open bars) and Ppt (closed bars) fractions after a 60-min incubation. Data are means ± SD (n = 3).
Figure 7. Effects of TF2A concentration on the interaction between TF2A and bile salt-PC micelles. (A) TF2A-dependent elevation of turbidity of micelle solutions containing 3 mM PC incubated with various concentrations of TF2A for 60 min at 37 °C. Data are means ± SD (n = 3). (B) Distribution of TF2A in the Sup (open bars) and Ppt (closed bars) fractions after a 60-min incubation. Data are means ± SD (n = 3). (C) Residual PC in the Sup fraction (% of total PC in the micelle solution) after a 60-min incubation with 0.3 and 0.5 mM TF2A. Data are means ± SD (n = 3). (D) Residual taurocholate in the Sup fraction (% of total taurocholate in the micelle solution) after a 60-min incubation with 0.3 and 0.5 mM TF2A. Data are means ± SD (n = 3). *Significantly lower than control (p < 0.05).
Scheme 1. Hypothesized interaction of TF2A with PC vesicles and the effects of subsequent addition of taurocholate.
Scheme 2. Hypothesized interaction of TF2A with taurocholate-PC micelles.
