Figures & data
Figure 1. Overview of the lycopene biosynthesis pathway in P. pastoris.
HMGR: HMG-CoA reductase; HMGS: HMG-CoA synthase; GGPPS (crtE): Geranylgeranyl diphosphate synthase; crtB: Phytoene synthase; crtI: Phytoene desaturase. The heterologous genes crtE, crtI, and crtB are from C. glutamicum ATCC13032; multiple reaction steps were indicated by dashed arrows. The heterogeneous biosynthetic pathways were highlighted with red ellipses.
Table 1. Plasmids used in this study.
Table 2. Yeast strains used in this study.
Figure 2. Flowchart of recombinant yeast strain construction in this study.
Figure 3. Cell growth and lycopene contents of engineered P. pastoris strains.
(a) The cell dry weight of the PAOX1/L and PGAP/L strains cultured in BMMY medium, measured at 0, 24, 48, 72, 96 and 120 h. (b) The lycopene contents of the PAOX1/L and PGAP/L strains cultured in BMMY for 5 days with 1% methanol addition and 1% glucose addition every 24 h, respectively.
Figure 4. Production of lycopene by engineered P. pastoris strains.
(a) Analysis of lycopene production of engineered P. pastoris strains, including L1, HL, HHL, GHHL and GGHHL. Strains were cultured in BMMY for 5 days with 1% methanol addition every 24 h . (b) The comparison of the lycopene production of different strains.
Figure 5. High-density fermentation for lycopene production.
The lycopene biomass accumulation of strains L1 (a) and GGHHL (b) during the fed batch fermentation. The high-density fermentation of the recombinant P. pastoris strain was performed in a 3 L fed-batch fermentation containing BSM.
