Autophagy promotion enhances the protective effect of Morroniside on human OA chondrocyte

Figures & data

Table 1. Specific primer sequences for qPCR.

Gene	Forward (5ʹ-3ʹ)	Reverse (5ʹ-3ʹ)
Atg5	GATGGGATTGCAAAATGACAGA	GAAAGGTCTTTCAGTCGTTGTC
Atg7	TGTATAACACCAACACACTCGA	GGCAGGATAGCAAAACCAATAG
BECN1 PCNA COL2 ACAN	ATCTAAGGAGCTGCCGTTATAC TAATTTCCTGTGCAAAAGACGG CAAGAACAGCATTGCCTATCTG GATCCTTACCGTAAAGCCCATC	CTCCTCAGAGTTAAACTGGGTT AAGAAGTTCAGGTACCTCAGTG GATAACAGTCTTGCCCCACTTA CTCCAGTCTCATTCTCAACCTC
GAPDH	ACCACAGTCCATGCCATCAC	TCCACCACCCTGTTGCTGTA

Figure 1. Morroniside inhibits the autophagic activity of chondrocytes.

Figure 2. The inhibition of PI3K/AKT/mTOR signaling reverses the inhibition of Morroniside on chondrocyte autophagy.

Figure 3. The inhibition of PI3K/AKT/mTOR signaling does not affect the protection of Morroniside on chondrocytes.

Figure 4. Overexpression of autophagy genes enhanced the protection of Morroniside on chondrocytes.

Figure 5. The schematic diagram representing the working model in our study.

Data availability statement

The data described in this article are openly available in the Open Science Framework at DOI:10.17605/OSF.IO/TPA6U.