Abstract
Polymannose (PM) having a weight-average molar mass (Mw) of 30–53 kDa was synthesized by the polycondensation of mannose using phosphorous acid as the catalyst and characterized by various techniques such as NMR, IR, GPC and polarimetry. 2D NMR results confirmed the presence of (1 → 6)-linked α-D-mannose residues as backbone with O-3 and O-2 substituted linear or branched chains in PM. Amphotericin B (AmB) was conjugated to periodate-oxidized PM through Schiff’s linkages at 20 wt% concentration. The AmB-PM conjugates were highly soluble in phosphate buffered saline (180–250 mg/mL), exhibited negligible hemolytic potential to human erythrocytes even at a concentration of 200 μg/mL (equivalent to ~40 μg/mL AmB) and were non-toxic to human embryonic kidney (HEK293T) cells even at a concentration of 250 μg/mL (equivalent to ~50 μg/mL AmB). The minimum inhibitory concentration of the AmB-PM conjugates against C. albicans, C. parapsilosis and C. neoformans was in the range of 0.5–1.0 μg/mL. Mannose receptors are widely expressed on myeloid cells such as macrophages, neutrophils, and dendritic cells. Therefore, apart from treating fungal infections, AmB-PM conjugates also may have therapeutic potential for the treatment of macrophage-associated diseases such as leishmaniasis where mannose receptors are overexpressed.
Acknowledgements
APF thanks IIT Madras for a post-doctoral fellowship. The authors thank Sophisticated Analytical Instrument Facility of IIT Madras for the characterization studies, Prof Rayala Suresh Kumar for his help with the cell culture studies and Dr P. Anbarasan of the Department of Chemistry, IIT Madras for interpretation of NMR data.