https://orcid.org/0000-0002-7081-2595
Figures & data
Figure 1. Lipid-dependent pathways in the regulation of platelet functions. The figure highlights the major lipid-mediated mechanisms described so far in blood platelets. The different colors of the boxes point out different functions or lipid families. Thromboxane A2 is a potent proaggregatory and vasoconstrictor, platelet-activating factor can stimulate platelet aggregation and activate immune cells, lysophosphatidic acid can stimulate platelet aggregation and induce cell proliferation, several eicosanoids are potent regulators of inflammation, sphingosine-1-phosphate is a pleiotropic lipid mediator involved in a large array of processes including inflammation, immunity, endothelial barrier integrity or tumorigenesis, phosphoinositides are key actors in intracellular signal transduction leading to platelet secretion, adhesion and aggregation, phosphatidic acid is involved in intracellular signal transduction [Citation7–9]. PS, phosphatidylserine; IP3, inositol trisphosphate; DAG, diacylglycerol; T×A2, thromboxane A2; PI(3,4,5)P3, phosphatidylinositol 1,4,5 trisphosphate; PI3P, phosphatidylinositol 3 monophosphate.
![Figure 1. Lipid-dependent pathways in the regulation of platelet functions. The figure highlights the major lipid-mediated mechanisms described so far in blood platelets. The different colors of the boxes point out different functions or lipid families. Thromboxane A2 is a potent proaggregatory and vasoconstrictor, platelet-activating factor can stimulate platelet aggregation and activate immune cells, lysophosphatidic acid can stimulate platelet aggregation and induce cell proliferation, several eicosanoids are potent regulators of inflammation, sphingosine-1-phosphate is a pleiotropic lipid mediator involved in a large array of processes including inflammation, immunity, endothelial barrier integrity or tumorigenesis, phosphoinositides are key actors in intracellular signal transduction leading to platelet secretion, adhesion and aggregation, phosphatidic acid is involved in intracellular signal transduction [Citation7–9]. PS, phosphatidylserine; IP3, inositol trisphosphate; DAG, diacylglycerol; T×A2, thromboxane A2; PI(3,4,5)P3, phosphatidylinositol 1,4,5 trisphosphate; PI3P, phosphatidylinositol 3 monophosphate.](/cms/asset/eb5f1302-41fd-4d1f-a0c7-7a5704cc1416/iplt_a_2182180_f0001_oc.jpg)
Figure 2. Detection of the molecular species of phosphatidylinositol monophosphate isomers by a LC/MS/MS method. The figure illustrates the separation of phosphatidylinositol monophosphate regioisomers (zoom of the total ionic current of an equimolar mixture of PI3P, PI4P and PI5P), using a chiral column as described recently [Citation14,Citation15]. The detection of the molecular species of the different phosphatidylinositol monophosphates (fatty acyl composition: C38:4>C38:3>other species) can be achieved by LC/MS/MS as schematically shown in the figure. A similar analysis can be performed for phosphatidylinositol bisphosphate regioisomers (PI(3,4)P2, PI(3,5)P2 and PI(4,5)P2).
![Figure 2. Detection of the molecular species of phosphatidylinositol monophosphate isomers by a LC/MS/MS method. The figure illustrates the separation of phosphatidylinositol monophosphate regioisomers (zoom of the total ionic current of an equimolar mixture of PI3P, PI4P and PI5P), using a chiral column as described recently [Citation14,Citation15]. The detection of the molecular species of the different phosphatidylinositol monophosphates (fatty acyl composition: C38:4>C38:3>other species) can be achieved by LC/MS/MS as schematically shown in the figure. A similar analysis can be performed for phosphatidylinositol bisphosphate regioisomers (PI(3,4)P2, PI(3,5)P2 and PI(4,5)P2).](/cms/asset/c70ed130-f843-4d20-8c5d-957e5feb8daa/iplt_a_2182180_f0002_oc.jpg)