Figures & data
Figure 1. Recombinant Cry1Ab. Lane 1, Cry1Ab stained with Coomassie brilliant blue. Lane 2, recognition of Cry1Ab polyclonal rabbit anti-Cry1Ab IgG. Molecular weight markers are indicated as kilodalton.
Figure 2. Antibody responses to Cry1 in adults from the USA (N = 100), Kenya (N = 100), and PNG (N = 100). Putative negative controls were from 20 Kenyan children aged 6–24 months. Polyclonal rabbit anti-Cry1Ab IgG (1:2000) served as a positive control. The quantitative cutoff value (mean of negative control cohort +3 SD), which defines the presence or absence of Cry1Ab-specific IgG, is indicated by the dotted line. All sera samples were diluted 1:200.
Figure 3. Cry1Ab-specific IgG Western blotting with sera from the highest ELISA responder of each population. Lane 1, Western Blot of Cry1Ab recognized by alkaline phosphatase-conjugated polyclonal rabbit anti-Cry1Ab IgG. Lanes 2–4, sera with the highest ODs (from ) from PNG, Kenya, and the USA, respectively. Lanes 5–7, sera negative for anti-Cry1Ab IgG (below cutoff threshold in ). Western blots were probed with sera diluted 1:200. Lane 1 is Cry1 probed with rabbit polyclonal and Cry1 antibodies.
