ABSTRACT
We researched clinically relevant antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) in environmental samples from a recreation club in Brazil. A total of 172 amplicons (105 from soil and 67 from water) of 26 ARGs (20 among the soil and water samples; four only in soil samples; two only in water samples) were detected. Nine MGEs were detected, including plasmids and class 1 integron. The absolute abundance of the mcr-3 gene ranged from 1.12 × 102 to 1.81 × 103 copies/mL−1 in water samples. The rapid spread of mcr-like genes in several sources has generated a huge concern to public health. Accordingly, understanding of antimicrobial resistance, carry out surveillance studies may contribute to tackle antimicrobial resistance. As the environmental samples were collected from a popular recreation club in Brazil, this study points out to the risk and exposure to clinically relevant ARGs, especially to mcr-3 and mcr-7.1 genes.
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Acknowledgments
The authors thank Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) [grant no. 2018/01890-3] and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) [grant no. 88882.180855/2018-01, 88882.180868/2018-01, 88887.314388/2019-00, and Finance code 001] for fellowships.
Disclosure statement
The authors declare no competing interests.
Ethical approval
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Conflicts of interest
The authors declare no conflict of interest.
Supplementary material
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