Effects of Bacillus subtilis PB6 supplementation on productive performance, egg quality and hatchability in broiler breeder hens under commercial farm condition

Figures & data

Table 1. Ingredients and nutrient content of the basal diets^a.

Ingredients	Female breeder (57–63 weeks of age)	Male breeder (57–63 weeks of age)
Corn grain	703.2	704.4
Soybean meal (44%)	150.6	65
Wheat bran	50.45	195.45
Di calcium phosphate	6.4	7.8
Oyster shell	75.8	14.5
Common salt	3.4	3.3
Sodium bicarbonate	1	1
Vitamin supplement^b	2.5	2.5
Mineral supplement^c	2.5	2.5
dl-Methionine	1.5	1
l-Lysine HCl	0	0.2
L-Threonine	0.3	0
Potassium carbonate	2	2
NSPase^d	0.3	0.3
Phytase^e	0.05	0.05
Sum	1000	1000
MEn (kcal/kg)	2800	2800
Crude protein	13	11.5
Calcium	3.2	0.90
Available phosphorus	0.32	0.35
Na	0.18	0.18
Dig. Lys	0.57	0.44
Dig. Met	0.33	0.28
Dig. Met + Cys	0.54	0.47
Dig. Thr	0.47	0.36

^a Diets formulated according to ROSS 308 management guide (2016).

^b Vitamin premix provided the following per kilogram of diet: vitamin A: 11000 IU; cholecalciferol: 3500 IU; vitamin E:100 IU; vitamin k3: 5 mg; vitamin B12: 0.03 mg; biotin: 0.25 mg; folacin: 2 mg; niacin: 55 mg; pantothenic acid: 15 mg; pyridoxine: 4 mg; riboflavin: 12 mg; thiamine: 3 mg.

^c Mineral premix provided the following per kilogram of diet: choline (as choline chloride): 300 mg, copper (as cupric sulfate 5H₂O): 10 mg; iodine (as calcium iodide): 2 mg; iron (as ferrous sulfate 4H₂O): 50 mg; manganese (as manganese sulfate): 120 mg; selenium (as sodium selenate): 0.3 mg, and zinc (as zinc oxide) 110 mg.

^d A combination of beta-glucanase (5 × 10⁶ U/kg), cellulase (5 × 10⁶ U/kg), alpha-amylase (2 × 10⁶ U/kg), protease (2 × 10⁶ U/kg), xylanase (2 × 10⁷ U/kg).

^e Added 50 g/ton, supplied 500 FTU phytase activity per kg feed.

Figure 1. Effect of Bacillus subtilis PB6 supplementation on egg weight of broiler breeder hens during 57–63 weeks of age. Values are presented as means ± Standard error.

Figure 2. Effect of Bacillus subtilis PB6 supplementation on egg production of broiler breeder hens during 57–63 weeks of age. values are presented as means ± sem; means lacking a common letter (a–b) differ significantly (P < 0.05).

Figure 3. Effect of Bacillus subtilis PB6 supplementation on body weight of broiler breeder hens during 57–63 weeks of age. Values are presented as means ± Standard error.

Figure 4. Effect of Bacillus subtilis PB6 supplementation on total mortality of broiler breeder hens.

Figure 5. Effect of Bacillus subtilis PB6 supplementation on cracked eggs during 57–63 weeks of age. Values are presented as means ± Standard error.

Figure 6. Effect of Bacillus subtilis PB6 supplementation on dirty eggs during 57–63 weeks of age. Values are presented as means ± Standard error.

Figure 7. Effect of Bacillus subtilis PB6 supplementation on eggshell thickness of broiler breeder hens during 60–63 weeks of age. Values are presented as means ± Standard error; means lacking a common letter (a–b) differ significantly (P < 0.05).

Figure 8. Effect of Bacillus subtilis PB6 supplementation on egg haugh unit of broiler breeder hens during 61–63 weeks of age. Values are presented as means ± Standard error.

Figure 9. Effect of Bacillus subtilis PB6 supplementation on hatching eggs during 57–63 weeks of age. Values are presented as means ± Standard error.

Figure 10. Effect of Bacillus subtilis PB6 supplementation on hatchability during 57–63 weeks of age. Values are presented as means ± Standard error; means lacking a common letter (a–b) differ significantly (P < 0.05).

Table 2. Effect of Bacillus subtilis PB6 supplementation on faecal bacterial count (Log 10 CFU/g) of broiler breeder hens at 63 week.

	Faecal bacterial count
	E. coli	Lactobacilli
Control	7.66	7.81
Bacillus subtilis PB6	7.94	7.64
P value	0.139	0.210
sem	0.012	0.036

Table 3. Effect of Bacillus subtilis PB6 supplementation on faecal score and litter moister at the end of the experiment.

	Faecal score				Litter moisture (%)
Age (weeks)	Control	Bacillus subtilis PB6	P value	sem	Control	Bacillus subtilis PB6	P value	sem
62	1.5^a	1^b	0.001	0.35	51	50	0.4198	1.63
63	1.5^a	1^b	0.003	0.35	50.5	50	0.5490	0.35

Faecal score: 1 = good, 2 = semi watery and 3 = watery, 30 observations at 10 different locations. Litter moisture: measured at 10 different locations.

^a,b Means with different superscripts in a row differ significantly (P < 0.05).

Table 4. Effect of Bacillus subtilis PB6 supplementation on blood parameters and fecal bacterial count of broiler breeder hens at 63 weeks.

	Blood parameters
	Heterophil: Lymphocyte	Cholesterol (mg/dl)
Control	0.643^a	213.66^b
Bacillus subtilis PB6	0.557^b	245.05^a
P value	0.001	0.0001
sem	0.004	16.61

^a,b Means with different superscripts in a column differ significantly (P < 0.05).

Figure 11. Effect of Bacillus subtilis PB6 supplementation on feed cost (US $) per hatchling number in broiler breeder hens.