Algal biomass: A sustainable, economical and renewable approach for microbial production of pectinolytic enzymes using submerged and solid state fermentation techniques

Abstract

In the past decade, algal waste has been used as useful natural resource for production of enormous range of products that have wide economical and commercial importance. Pectinases are group of enzymes that have wide commercial applications. Hence, current study was designed to utilize algal biomass for the production of pectinases using submerged (SmF) and solid state fermentation (SSF) techniques. Different algal sources including brown (Dictyopteris polypodioides, Sargassum wightii and Dictyopteris divaricata) and green algae (Ulva lactuca and Codium tomentosum) were used and U. lactuca was found to be the most suitable substrate. Several bacterial and fungal strains were screened and among them Bacillus licheniformis KIBGE-IB4 was selected based on maximum pectinase production. SmF and SSF were studied utilizing U. lactuca as a substrate and results revealed that enzyme production was favoured by SmF (2457 ± 3.31 U mg⁻¹) as compared to SSF (1432 ± 1.46 U mg⁻¹). Parametric optimization of pectinase production indicated that B. licheniformis KIBGE-IB4 requires 10.0 g L^–1 U. lactuca as a biomass in the medium with a pH 7.0 when incubated at 37 °C for 24 hours. Likewise, production of pectinase using algal resource was also compared with that of the conventional agricultural biomass and it was observed that when U. lactuca was used, the selected bacterial isolate produced a higher yield of enzyme than sugarcane bagasse and rice husk. Hence, it is anticipated that algal biomass can be efficiently utilized as an environmental friendly bioresource for the production of industrially important hydrolytic enzymes.

Keywords:

• Algae
• fermentation
• marine biomass
• seaweed
• pectinase

Disclosure statement

The publication of this manuscript is approved by all authors and they do not have any conflict of interest regarding any financial, personal or other relationships with any other people or organizations.