Relationship between expression of glucocorticoid receptor isoforms and glucocorticoid resistance in immune thrombocytopenia

Figures & data

Table 1 Clinicopathological features of ITP patients and controls

			PLT (×10^9/l)
Case No.	Sex (M/F)	Median age	Before treatment	After treatment
ITP (n = 49)	16/33	34 years	—	—
Control (n = 31)	11/20	34 years	—	—
GC-sensitive (n = 22)	5/17	30.5 years	7.4 (5.15–12.15)	113.5 (64.9–153.5)
GC-resistant (n = 10)	5/5	47.5 years	8.4 (5.65–13.9)	17.2 (8.07–24.4)

Table 2 Primers of GRα, GRβ, GRγ, GRP, and GAPDH

Gene	Sequence of primer	Length of product
GR-α	Forward: CTATGCATGAAGTGGTTGAAAA Reverse: TTTCAGCTAACATCTCGGG	96 bp
GR-β	Forward: GAAGGAAACTCCAGCCAGAA Reverse: CCACATAACATTTTCATGCATAGA	81 bp
GR-γ	Forward: TTCAAAAGAGCAGTGGAAGGTA Reverse: GGTAGGGGTGAGTTGTGGTAACG	264 bp
GR-P	Forward: GCTGTGTTTTGCTCCTGATCTGA Reverse: TGACATAAGGTGAAAAGGTGTTCTACC	194 bp
GAPDH	Forward: GGACCTGACCTGCCGTCTAG Reverse: TAGCCCAGGATGCCCTTGAG	99 bp

Table 3 Expression of four GR isoform mRNAs in ITP patients and controls

	ITP n = 49		Control n = 31
GR	Median	25–75%	Median	25–75%
GRα	1.19*	0.48–3.06	1.06	0.36–1.37
GRβ	0.08*	0.01–0.46	0.04	0–0.08
GRγ	0.27	0.09–0.91	0.40	0.18–0.83
GRP	0.48	0–2.87	0.74	0.25–3.68
GR	5.25*	2.02–13.12	2.88	1.27–5.59

*P < 0.05.

Figure 1 Melting curve of GAPDH, GRα, GRβ, GRγ, and GRP.

Figure 2 Image of an electrophoresis gel of PCR products of GRα, β, γ, and P. Single band of PCR products showed the absence of other non-specific products among the PCR products.

Figure 3 (A) Expression of GRα, β, γ, and P mRNA in the ITP and control groups. (B) Correlations between GRα and GRP in ITP patients and controls.

Figure 4 Expression of GRα, β, γ, and P mRNA in the GC-sensitive group, GC-resistant group, and the control group.

Table 4 Expression of four GR isoform mRNAs in GC-sensitive, GC-resistant, and control groups

	Sensitive n = 22		Resistant n = 10		Control n = 31
GR	Median	25–75%	Median	25–75%	Median	25–75%
GRα	1.11	0.31–2.48	0.85*	0.32–3.06	1.06	0.36–1.37
GRβ	0.04	0–0.63	0.03	0–0.16	0.04	0–0.08
GRγ	0.20	0.07–1.06	0.42	0.08–14.38	0.40	0.18–0.83
GRP	0.40	0–0.97	0.30	0–3.21	0.74	0.25–3.68

*P < 0.05.

Figure 5 GRα protein expression in the GC-sensitive group (n = 6), GC-resistant group (n = 6), and the control group (n = 6). Note:*P < 0.05.