Lower expression of PD-1 and PD-L1 in peripheral blood from patients with chronic ITP

Figures & data

Table 1 Clinical data of the ITP patients

No.	Sex	Age	Platelet count (×10^9/l)	WBC count (×10^9/l)	Refractory	Gene expression
1	F	42	3.0	4.5
2	F	32	3.0	5.1	Yes
3	M	40	5.0	6.17
4	F	25	33.0	9.71
5	M	35	46.0	7.63		Highest
6	F	32	1.0	8.1
7	M	27	4.0	5.0	Yes
8	M	24	4.0	4.45
9	F	37	7.0	7.4
10	F	42	19.0	12.74		Lowest
11	M	46	51.0	11.27
12	M	68	10.0	3.1	Yes
13	F	28	3.0	10.04
14	F	52	6.0	4.76
15	F	24	51	6.4
16	F	48	9.0	5.0
17	F	67	19.4	9.94	Yes
18	F	46	10.0	5.06
		38.5*	15.80 ± 17.32^†	7.02 ± 2.74^†

*Median.

^†Mean ± SD.

Table 2 List of primers used for real-time PCR

Primer	Sequence
PD-1-for	5′-CTCAGGGTGACAGAGAGAAG-3′
PD-1-rev	5′-GACACCAACCACCAGGGTTT-3′
PD-L1-for	5′-TATGGTGGTGCCGACTACAA-3′
PD-L1-rev	5′-TGCTTGTCCAGATGACTTCG-3′
CTLA-4-for	5′-AGACCTGAACACCGCTCCC-3′
CTLA-4-rev	5′-GTCAGCCTGCCGAAGCACT-3′
β2M-for	5′-TACACTGAATTCACCCCCAC-3′
β2M-rev	5′-CATCCAATCCAAATGCGGCA-3′

Figure 1 The expression levels of PD-1, PD-L1, and CTLA-4 in ITP patients and healthy individuals (HIs). Significantly decreased expression of PD-1 (A) and PD-L1 (B) was found, while the CTLA-4 expression level in the ITP group (C) appeared to be similar to that of the control group. The highest expression of PD-1, PD-L1, and CTLA-4 was found in the same ITP sample (case 5) (red points), and the lowest expression for all three genes was also found in the same ITP sample (case 10) (blue points).

Figure 2 The correlation between the expression levels of PD-1, PD-L1, and CTLA-4 in ITP patients and healthy individuals (HIs). (A) PD-1 vs. PD-L1 in ITP, (B) PD-1 vs. PD-L1 in HI; (C) PD-1 vs. CTLA-4 in ITP; (D) PD-1 vs. CTLA-4 in HI, (E) PD-L1 vs. CTLA-4 in ITP; (F) PD-L1 vs. CTLA-4 in HI.