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Articles

Microbiology in Water-Miscible Metalworking Fluids

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Pages 1147-1171 | Received 17 Feb 2020, Accepted 29 Apr 2020, Published online: 14 Oct 2020

Figures & data

Figure 1. Microorganisms are highly diverse organisms originating from all domains of life—Archaea, Eukarya, and Bacteria—plus viruses (not shown). These three domains differ in cellular construction, biochemistry, and genetics. (A)The three-domain model postulates that Eukarya and Archaea shared a common ancestor, whereas (B) the two-domain model claims that Eukarya actually descends from Archaea. It remains unclear which model is correct.

Figure 1. Microorganisms are highly diverse organisms originating from all domains of life—Archaea, Eukarya, and Bacteria—plus viruses (not shown). These three domains differ in cellular construction, biochemistry, and genetics. (A)The three-domain model postulates that Eukarya and Archaea shared a common ancestor, whereas (B) the two-domain model claims that Eukarya actually descends from Archaea. It remains unclear which model is correct.

Figure 2. Some bacteria live as solitary and planktonic organisms, at least for a while. However, (A) this lifestyle has disadvantages and (B) the majority seeks refuge on surfaces, forming biofilms. This lifestyle confers many advantages on their inhabitants protecting them from the environment. Biofilms represent a much higher level of organization than single cells do.

Figure 2. Some bacteria live as solitary and planktonic organisms, at least for a while. However, (A) this lifestyle has disadvantages and (B) the majority seeks refuge on surfaces, forming biofilms. This lifestyle confers many advantages on their inhabitants protecting them from the environment. Biofilms represent a much higher level of organization than single cells do.

Figure 3. Biofilms in MWF systems may vary in size and maturity. (A) Very young biofilms are easily removable, whereas (B) mature ones offer a lot of resistance to cleaning agents. (C), (D) Molds like to settle in places outside the waterline, and the surfaces covered may be very small at the beginning but offer them a chance to distribute all over, using spores as transportation devices.

Figure 3. Biofilms in MWF systems may vary in size and maturity. (A) Very young biofilms are easily removable, whereas (B) mature ones offer a lot of resistance to cleaning agents. (C), (D) Molds like to settle in places outside the waterline, and the surfaces covered may be very small at the beginning but offer them a chance to distribute all over, using spores as transportation devices.

Figure 4. Machine shop ventilation. Air change rate is 10 h−1 in both facilities. (A) Air enters low and exits high so that the facility is well ventilated. (B) Air intakes are above the breathing zone. Consequently, the net air exchange rate belies stagnation in the breathing zone.

Figure 4. Machine shop ventilation. Air change rate is 10 h−1 in both facilities. (A) Air enters low and exits high so that the facility is well ventilated. (B) Air intakes are above the breathing zone. Consequently, the net air exchange rate belies stagnation in the breathing zone.

Figure 5. Exotoxins and endotoxins. (A) Exotoxins are diffusible proteins secreted into the surrounding medium by mostly gram-positive bacteria. (B) Classic endotoxins are part of the outer membrane of the cell wall of gram-negative bacteria. They are liberated upon lysis.

Figure 5. Exotoxins and endotoxins. (A) Exotoxins are diffusible proteins secreted into the surrounding medium by mostly gram-positive bacteria. (B) Classic endotoxins are part of the outer membrane of the cell wall of gram-negative bacteria. They are liberated upon lysis.