ABSTRACT
Gelatin is a highly purified animal protein of pig, cow, and fish origins and is extensively used in food, pharmaceuticals, and personal care products. However, the acceptability of gelatin products greatly depends on the animal sources of the gelatin. Porcine and bovine gelatins have attractive features but limited acceptance because of religious prohibitions and potential zoonotic threats, whereas fish gelatin is welcomed in all religions and cultures. Thus, source authentication is a must for gelatin products but it is greatly challenging due to the breakdown of both protein and DNA biomarkers in processed gelatins. Therefore, several methods have been proposed for gelatin identification, but a comprehensive and systematic document that includes all of the techniques does not exist. This up-to-date review addresses this research gap and presents, in an accessible format, the major gelatin source authentication techniques, which are primarily nucleic acid and protein based. Instead of presenting these methods in paragraph form which needs much attention in reading, the major methods are schematically depicted, and their comparative features are tabulated. Future technologies are forecasted, and challenges are outlined. Overall, this review paper has the merit to serve as a reference guide for the production and application of gelatin in academia and industry and will act as a platform for the development of improved methods for gelatin authentication.
Acknowledgment
This work is supported by the University of Malaya's Grand Challenge fund no. GC001A-14SBS awarded to M.E. Ali.
Authors' contribution and declaration
Md. Eaqub Ali declares that he generated the idea, managed the funds, and guided Sharmin Sultana to draft the manuscript, and he has no competing interests in the publication of this paper.
Sharmin Sultana declares that she drafted the manuscript, and she has no competing interests in the publication of this paper.
Sharifah Bee Abd Hamid declares that she supervised Sharmin Sultana and edited the manuscript, and she has no competing interests in the publication of this paper.
M.A. Motalib Hossain declares that he helped Sharmin Sultana with drafting the manuscript, and he has no competing interests in the publication of this paper.
Wageeh A. Yehya declares that he edited the manuscript, and he has no competing interests in the publication of this paper.
Md. Abdul Kader declares that he edited the manuscript, and he has no competing interests in the publication of this paper.
Suresh K. Bhargava declares that he edited the manuscript, and he has no competing interests in the publication of this paper.