Thyroid transcriptomic profiling reveals the differential regulation of lncRNA and mRNA related to prolificacy in small tail han sheep with FecB BB genotype

Figures & data

Table 1. Primer sequences.

Gene name	Classification	Sequence (5’-3’)
LNC_010836	F	AACCCACAAAGAGAAGAGCATTCCC
	R	GCGAGCCTGTAGAAGATGAGAAAGC
LNC_008165	F	AGGTCTGGTAGGTGCTTCTTCGG
	R	GAGTGAGGTGGTGACTGCTGTTG
LNC_015926	F	CTCACAGGCACAGATCAGCTAACC
	R	GAACCTCACCAGACGCAGTAACAG
RAG1	F	AGGAGGAAGGTGGAGATGTGAAGTC
	R	CTGCTTGTCTGTGCTCATTTCTTGC
MEF2C	F	TTCCACCAGGCAGCAAGAATACG
	R	GGTAGCCAATGACTGAGCAGACTG
AP2M1	F	GTATCGCACCACCAAGGACATCATC
	R	AGCCAGTAGTGAGGGTTTGAAGTTG
RPL-19	F	ATCGCCAATGCCAACTC
	R	CCTTTCGCTTACCTATACC

Table 2. Summary Of raw reads after the quality control and reference genome mapping.

Sample name	Raw reads	Clean reads	Clean bases	Mapped reads	Mapping Rate (%)	Q30(%)
MM_F_T_1	97711458	94718122	14.21G	86628641	91.46	95.12
MM_F_T_2	96088926	93452790	14.02G	85202534	91.17	93.78
MM_F_T_3	92644672	90743952	13.61G	83435512	91.95	94.41
MM_L_T_1	91878326	87721536	13.16G	78486694	89.47	92.52
MM_L_T_2	97154738	92621496	13.89G	83663515	90.33	92.74
MM_L_T_3	98227970	94831240	14.22G	85875742	90.56	93.03

Figure 1. The volcano plot shows the expression of lncRNAs (A) and (B) mRNAs, and the heatmap shows the expression pattern of DELs (C) and DEGs (D).

Figure 2. The top 10 enriched GO terms for the identified genes targeted by DELs (A) and DEGs (B) in MM_FT and MM_LT.

Figure 3. The 20 enriched KEGG pathways for the identified genes targeted by DELs (A) and DEGs (B) in MM_FT and MM_LT.

Figure 4. Co-expression of lncRNAs-mRNAs after genes targeted by DELs coincided with DEGs in MM_FT and MM_LT. Red (increased expression during the follicular phase) and green (decreased expression during the follicular phase) indicate up or down-regulation, respectively.

Figure 5. RT-qPCR validation of DELs (A) and DEGs (B) identified by RNA-seq in MM_FT and MM_LT.

Data availability statement

Experimental animals in this study were authorized by the Science Research Department (in charge of animal welfare issues) of the Institute of Animal Science, Chinese Academy of Agricultural Sciences (IAS-CAAS; Beijing, China). Additionally, ethical approval of animal survival and the sample collection was given by the animal ethics committee of IAS-CAAS (No. IAS2019-49).