Sodium N-lauryl amino acids derived from silk protein can form catanionic aggregates with cytarabine as novel anti-tumor drug delivery systems

Figures & data

Figure 1. (a) The apparent morphology of catanionic aggregation in solutions of SSS-CH. (b) The TEM photos of SSS-CH catanionic aggregates. X₁ is expressed as the mass fraction of CH (X₁=m_CH/m_(SSS+CH)).

Figure 2. Particle size distribution diagram of SSS-CH catanionic aggregate solutions with different X₁ values.

Figure 3. (a) Conductivity diagram of SSS-CH catanionic aggregate solutions with different X₁ values. (b) Turbidity values for SSS-CH catanionic aggregate solutions with different X₁ values.

Table 1. The zeta potential value of SSS-CH catanionic aggregate solutions with different X₁ values.

X1	Zeta potential/mV
0.1	−61.7
0.2	−47.8
0.3	−44.6
0.4	−26.5
0.5	−24.8
0.6	−18.8
0.7	−12.9
0.8	−9.4
0.9	−5.7

Figure 4. Drug release results for SSS-CH catanionic aggregate solutions with different X₁ values (n = 3).

Figure 5. Cell viability in response to different concentrations of SSS incubated with CEM and Ramous cells (n = 3). (a, b) Represents the effect of SSS on the survival rate of CEM and Ramous cells, respectively.

Figure 6. Cell morphologies of CEM (upper) and Ramous (lower) cells incubated with 100 µg/mL samples for 24 h and 48 h. Magnification: ×100.

Figure 7. The inhibitory rates of different concentrations of CH and SSS-CH on CEM cells (n = 3), (a): 24 h, (b): 48 h, *Indicates the difference compared with the blank group, *p<.05, significant difference, **p<.01, very significant difference, ***p<.001, extremely significant difference.

Figure 8. Inhibitory rates of different concentrations of CH and SSS-CH on Ramous cells (n = 3). (a): 24 h, (b): 48 h. *Indicates the difference compared with the blank group, *p<.05, significant difference, **p<.01, very significant difference, ***p<.001, extremely significant difference.

Data availability

The datasets used and/or analyzed during the current study as well as analysis scripts are available from the corresponding author on reasonable request.