ROS-sensitive calcipotriol nano-micelles prepared by methoxypolyethylene glycol (mPEG) – modified polymer for the treatment of psoriasis

Figures & data

Figure 1. (A) Synthetic route of mPEG-SS; (B) ¹H-NMR spectra of mPEG, mPEG-SH, and mPEG-SS; (C) MS for mPEG-SS; (D) CMC of mPEG-SS with and without H₂O₂.

Figure 2. (A) Microstructure of PSC without H₂O₂ and Tyndall phenomenon (upper right corner) of PSC; (B) Particle size distribution of PSC without H₂O₂; (C) H₂O₂ effect on PSC microstructure (24 h); (D) H₂O₂ effect on PSC particle size; (E) Particle size curve with time; (F) Time-dependent fluorescence of PSF incubated without and with H₂O₂; (G) Histogram of time-dependent fluorescence; (H) Depolymerization schematic diagram of PSC.

Figure 3. (A) Cytotoxicity of mPEG-SS, CPT, and PSC on HaCaT without H₂O₂; (B) Cytotoxicity of mPEG-SS, CPT, and PSC on HaCaT under H₂O₂ condition (*P < 0.05 compared with the control group, n = 3).

Figure 4. (A) PSC scavenging extracellular ROS; (B) PSC scavenging intracellular ROS; (C) Cellular images of PSC scavenging intracellular ROS of the normal group; (D) H₂O₂ group; (E) H₂O₂ + mPEG-ss group; (F) H₂O₂ + free CPT group; (G) H₂O₂ + PSD; (H) Positive control group; (scale bar: 20 μm; **P < 0.01; *P < 0.05 compared with the control group, n = 3).

Figure 5. (A) Molding and drugging procedure of psoriasis mice; (B) PASI score (total, erythema, scaling, hypertrophy score); (C) Photographs of dorsal skin of each group of mice; (D) In vivo fluorescence imaging.

Figure 6. (A) H&E and VEGF staining; (B) In vivo ROS-scavenging effect; (C) Serum IL-6; (D) TNF-α level (**P < 0.01; *P < 0.05 compared with the control group, n = 3).